Restriction map for
pF1KB6013
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# NO CUT ENZYMES ( 111 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AfaI AflII AgeI AhdI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I EcoRI EcoRV
EcoT22I FbaI FspI HincII HindII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspBI SspI SwaI Tru9I
Tsp509I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 1699 GACGTC GACGT^C
AcyI 1699 GACGTC G[GA]^CG[CT]C
AflIII 328 ACATGT A^C[GA][CT]GT
Alw44I 649 GTGCAC G^TGCAC
AlwNI 1368 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 885 CCTGAGG CC^T[ACGT]AGG
ApaLI 649 GTGCAC G^TGCAC
AspI 1384 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 81 GGTGCC G^G[CT][GA]CC
BbrPI 1528 CACGTG CAC^GTG
BbsI 1689 GAAGAC GAAGAC(2/6)
BpuAI 1689 GAAGAC GAAGAC(2/6)
BsaAI 1528 CACGTG [CT]AC^GT[GA]
BsaHI 1699 GACGTC G[GA]^CG[CT]C
BsiEI 974 CGGCCG CG[GA][CT]^CG
BsmBI 199 GAGACG CGTCTC(1/5)
BsmFI 250 GGGAC GGGAC(10/14)
BspLU11I 328 ACATGT A^CATGT
BstAPI 1642 GCATCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 774 CCATGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 848 GGATCT [GA]^GATC[CT]
Bsu36I 885 CCTGAGG CC^T[ACGT]AGG
DsaI 665 CCATGG C^C[GA][CT]GG
EagI 974 CGGCCG C^GGCCG
EarI 1015 GAAGAG CTCTTC(1/4)
EclXI 974 CGGCCG C^GGCCG
Eco52I 974 CGGCCG C^GGCCG
Eco81I 885 CCTGAGG CC^T[ACGT]AGG
FseI 703 GGCCGGCC GGCCGG^CC
HgaI 169 GACGC GACGC(5/10)
Hin1I 1699 GACGTC G[GA]^CG[CT]C
HindIII 1666 AAGCTT A^AGCTT
Ksp632I 1015 GAAGAG CTCTTC(1/4)
MflI 848 GGATCT [GA]^GATC[CT]
NcoI 665 CCATGG C^CATGG
PflFI 1384 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 155 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1528 CACGTG CAC^GTG
PmlI 1528 CACGTG CAC^GTG
SapI 1015 GAAGAGC GCTCTTC(1/4)
TfiI 150 GAATC G^A[AT]TC
Tth111I 1384 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 155 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 848 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 744 GGATC GGATC(4/5)
848 GGATC
Aor51HI 51 AGCGCT AGC^GCT
1328 AGCGCT
AspHI 177 GAGCAC G[AT]GC[AT]^C
649 GTGCAC
AvaI 76 CTCGGG C^[CT]CG[GA]G
1431 CTCGGG
BalI 381 TGGCCA TGG^CCA
1610 TGGCCA
BseRI 1297 GAGGAG GAGGAG(10/8)
1420 GAGGAG
BsgI 858 CTGCAC GTGCAG(16/14)
1155 GTGCAG
BsiHKAI 177 GAGCAC G[AT]GC[AT]^C
649 GTGCAC
BsoBI 76 CTCGGG C^[CT]CG[GA]G
1431 CTCGGG
BsrBI 1576 CCGCTC CCGCTC(-3/-3)
1137 GAGCGG
BsrDI 804 CATTGC GCAATG(2/0)
1262 GCAATG
BstEII 660 GGTCACC G^GT[ACGT]ACC
795 GGTCACC
BstPI 660 GGTCACC G^GT[ACGT]ACC
795 GGTCACC
Eco47III 51 AGCGCT AGC^GCT
1328 AGCGCT
EcoNI 1310 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1494 CCTGGGCCAGG
EcoO65I 660 GGTCACC G^GT[ACGT]ACC
795 GGTCACC
MluNI 381 TGGCCA TGG^CCA
1610 TGGCCA
MscI 381 TGGCCA TGG^CCA
1610 TGGCCA
NaeI 127 GCCGGC GCC^GGC
704 GCCGGC
NgoMIV 127 GCCGGC G^CCGGC
704 GCCGGC
PpuMI 881 GGGTCCT [GA]G^G[AT]CC[CT]
1460 AGGTCCT
PvuII 811 CAGCTG CAG^CTG
832 CAGCTG
SbfI 182 CCTGCAGG CCTGCA^GG
1170 CCTGCAGG
SmlI 1486 CTCAAG C^T[CT][GA]AG
1705 CTCAAG
Sse8387I 182 CCTGCAGG CCTGCA^GG
1170 CCTGCAGG
TaqI 1037 TCGA T^CGA
1645 TCGA
TthHB8I 1037 TCGA T^CGA
1645 TCGA
XcmI 155 CCAACCTCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
254 CCAAGAGCTGCGTGG
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AluI ApaI AvaII
BanII BbvI BcnI BglI BmyI
BsaI BsaJI BsiYI BslI BsmAI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI SphI
StuI StyI TseI Tsp45I TspRI