Restriction map for
pF1KB3144
##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AflII AflIII
AgeI AhdI Alw44I ApaLI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BciVI BclI BfrI BlnI
BsaAI BsaBI BsiWI BspDI BspHI
BspLU11I BsrBI BsrDI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI Eam1105I
EcoO65I EcoRV EcoT22I FbaI FspI
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I Van91I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 132 TCCGGA T^CCGGA
AclI 1425 AACGTT AA^CGTT
AcsI 1277 GAATTC [GA]^AATT[CT]
AcyI 466 GGCGTC G[GA]^CG[CT]C
AocI 691 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1211 AGCGCT AGC^GCT
ApoI 1277 GAATTC [GA]^AATT[CT]
AscI 579 GGCGCGCC GG^CGCGCC
BbsI 285 GTCTTC GAAGAC(2/6)
BcgI 335 CGAGGGTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1288 CTAG C^TAG
BglII 995 AGATCT A^GATCT
BpuAI 285 GTCTTC GAAGAC(2/6)
BsaHI 466 GGCGTC G[GA]^CG[CT]C
BsaWI 132 TCCGGA [AT]^CCGG[AT]
BseAI 132 TCCGGA T^CCGGA
BsmBI 468 CGTCTC CGTCTC(1/5)
BsmI 1455 GAATGC GAATGC(1/-1)
Bsp1407I 914 TGTACA T^GTACA
BspEI 132 TCCGGA T^CCGGA
BsrGI 914 TGTACA T^GTACA
BssHII 580 GCGCGC G^CGCGC
Bsu36I 691 CCTGAGG CC^T[ACGT]AGG
Eco47III 1211 AGCGCT AGC^GCT
Eco81I 691 CCTGAGG CC^T[ACGT]AGG
EcoRI 1277 GAATTC G^AATTC
FseI 89 GGCCGGCC GGCCGG^CC
Hin1I 466 GGCGTC G[GA]^CG[CT]C
KspI 413 CCGCGG CCGC^GG
MaeI 1288 CTAG C^TAG
MroI 132 TCCGGA T^CCGGA
MseI 1085 TTAA T^TAA
PaeR7I 56 CTCGAG C^TCGAG
Psp1406I 1425 AACGTT AA^CGTT
SacI 196 GAGCTC GAGCT^C
SacII 413 CCGCGG CCGC^GG
SspBI 914 TGTACA T^GTACA
StuI 1169 AGGCCT AGG^CCT
Tru9I 1085 TTAA T^TAA
Tsp45I 1130 GTGAC ^GT[GC]AC
XhoI 56 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 603 GTAC GT^AC
915 GTAC
AlwNI 601 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
676 CAGCCACTG
AspHI 196 GAGCTC G[AT]GC[AT]^C
526 GTGCTC
BalI 932 TGGCCA TGG^CCA
1349 TGGCCA
BsaI 1003 GAGACC GGTCTC(1/5)
1541 GAGACC
BsiEI 11 CGGCCG CG[GA][CT]^CG
92 CGGCCG
BsiHKAI 196 GAGCTC G[AT]GC[AT]^C
526 GTGCTC
BstAPI 354 GCAGCTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
956 GCAACTCCTGC
BstYI 995 AGATCT [GA]^GATC[CT]
1304 AGATCC
EagI 11 CGGCCG C^GGCCG
92 CGGCCG
EarI 1656 CTCTTC CTCTTC(1/4)
1714 GAAGAG
EclXI 11 CGGCCG C^GGCCG
92 CGGCCG
Eco52I 11 CGGCCG C^GGCCG
92 CGGCCG
HaeII 744 GGCGCT [GA]GCGC^[CT]
1211 AGCGCT
Ksp632I 1656 CTCTTC CTCTTC(1/4)
1714 GAAGAG
MaeII 170 ACGT A^CGT
1426 ACGT
MaeIII 821 GTTAC ^GT[ACGT]AC
1130 GTGAC
MflI 995 AGATCT [GA]^GATC[CT]
1304 AGATCC
MluNI 932 TGGCCA TGG^CCA
1349 TGGCCA
MscI 932 TGGCCA TGG^CCA
1349 TGGCCA
MslI 453 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1569 CATTCTGATG
NcoI 740 CCATGG C^CATGG
1308 CCATGG
RsaI 603 GTAC GT^AC
915 GTAC
TaqI 57 TCGA T^CGA
334 TCGA
TthHB8I 57 TCGA T^CGA
334 TCGA
XcmI 731 CCAACAGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1517 CCAGCGACCACCTGG
XhoII 995 AGATCT [GA]^GATC[CT]
1304 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
AvaI AvaII BanI BanII BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
TseI Tsp509I TspRI