Restriction map for
pF1KB3289
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AgeI AhdI
AlwNI AocI Aor51HI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BalI BanI BbeI BbvCI
BcgI BciVI BcnI BglI BlnI
BlpI Bpu1102I BsaHI BsaI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT14I FauI FseI FspI HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TaqI
TthHB8I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflII 919 CTTAAG C^TTAAG
ApaI 75 GGGCCC GGGCC^C
AspI 534 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1820 GGATCC G^GATCC
BbrPI 985 CACGTG CAC^GTG
BfaI 1564 CTAG C^TAG
BfrI 919 CTTAAG C^TTAAG
BglII 1800 AGATCT A^GATCT
BsaAI 985 CACGTG [CT]AC^GT[GA]
BsaBI 802 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 506 CCCAGG C^C[ACGT][ACGT]GG
BsiEI 1956 CGGTCG CG[GA][CT]^CG
BspHI 633 TCATGA T^CATGA
BspMI 128 ACCTGC ACCTGC(4/8)
BstF5I 145 CATCC GGATG(2/0)
Cac8I 1875 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 956 GCGC GCG^C
DrdI 405 GACAGAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI 145 CATCC GGATG(9/13)
HaeII 955 GGCGCT [GA]GCGC^[CT]
HhaI 956 GCGC GCG^C
HinP1I 956 GCGC G^CGC
MaeI 1564 CTAG C^TAG
MamI 802 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1732 CAGCTG C[AC]G^C[GT]G
MwoI 303 GCAAATCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 1425 CATATG CA^TATG
PflFI 534 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 614 CCATCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 985 CACGTG CAC^GTG
PmlI 985 CACGTG CAC^GTG
PvuII 1732 CAGCTG CAG^CTG
RcaI 633 TCATGA T^CATGA
SacI 1710 GAGCTC GAGCT^C
ScaI 2117 AGTACT AGT^ACT
Tth111I 534 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 614 CCATCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 841 ACATGT A^C[GA][CT]GT
903 ACATGT
Alw44I 1964 GTGCAC G^TGCAC
2074 GTGCAC
ApaLI 1964 GTGCAC G^TGCAC
2074 GTGCAC
BanII 75 GGGCCC G[GA]GC[CT]^C
1710 GAGCTC
BbvI 189 GCAGC GCAGC(8/12)
69 GCTGC
BclI 353 TGATCA T^GATCA
717 TGATCA
BsmFI 403 GGGAC GGGAC(10/14)
464 GTCCC
BspLU11I 841 ACATGT A^CATGT
903 ACATGT
BstYI 1800 AGATCT [GA]^GATC[CT]
1820 GGATCC
DraII 75 GGGCCCT [GA]G^G[ACGT]CC[CT]
1108 AGGGCCT
EcoO109I 75 GGGCCCT [GA]G^G[ACGT]CC[CT]
1108 AGGGCCT
EcoT22I 1181 ATGCAT ATGCA^T
2062 ATGCAT
FbaI 353 TGATCA T^GATCA
717 TGATCA
Fnu4HI 69 GCTGC GC^[ACGT]GC
189 GCAGC
ItaI 69 GCTGC GC^[ACGT]GC
189 GCAGC
MaeII 35 ACGT A^CGT
986 ACGT
MflI 1800 AGATCT [GA]^GATC[CT]
1820 GGATCC
NsiI 1181 ATGCAT ATGCA^T
2062 ATGCAT
SapI 286 GAAGAGC GCTCTTC(1/4)
1527 GAAGAGC
SexAI 334 ACCAGGT A^CC[AT]GGT
395 ACCAGGT
TseI 69 GCTGC G^C[AT]GC
189 GCAGC
XhoII 1800 AGATCT [GA]^GATC[CT]
1820 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AspHI AvaII BbsI BmyI
BpmI BpuAI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrDI BsrI
BssKI BstNI Cfr13I DdeI DpnII
EarI EcoRI EcoRII HaeIII HinfI
HphI Ksp632I MaeIII MboI MboII
MnlI MseI MslI MvaI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI TfiI Tru9I Tsp45I
Tsp509I TspRI XmnI