Restriction map for
pF1KB9407
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# NO CUT ENZYMES ( 95 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BlnI BsaAI BsaBI BseAI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DrdI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
FseI FspI HaeII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NcoI
NdeI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 2685 GACGCC G[GA]^CG[CT]C
AseI 2667 ATTAAT AT^TAAT
AsnI 2667 ATTAAT AT^TAAT
AspI 1111 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglII 1616 AGATCT A^GATCT
BlpI 3545 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3545 GCTTAGC GC^T[ACGT]AGC
BsaHI 2685 GACGCC G[GA]^CG[CT]C
BsaWI 3176 ACCGGA [AT]^CCGG[AT]
BsiWI 125 CGTACG C^GTACG
BsmBI 3704 GAGACG CGTCTC(1/5)
BspHI 378 TCATGA T^CATGA
BsrBI 134 GAGCGG CCGCTC(-3/-3)
BsrFI 2850 GCCGGC [GA]^CCGG[CT]
BstAPI 3085 GCAGGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 3545 GCTTAGC GC^T[ACGT]AGC
Cfr10I 2850 GCCGGC [GA]^CCGG[CT]
DraI 2380 TTTAAA TTT^AAA
DraIII 2621 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2981 CCGTGG C^C[GA][CT]GG
EagI 2643 CGGCCG C^GGCCG
EclXI 2643 CGGCCG C^GGCCG
Eco52I 2643 CGGCCG C^GGCCG
EcoNI 3237 CCTAATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3092 ATGCAT ATGCA^T
Hin1I 2685 GACGCC G[GA]^CG[CT]C
NaeI 2850 GCCGGC GCC^GGC
NgoMIV 2850 GCCGGC G^CCGGC
NheI 1783 GCTAGC G^CTAGC
NsiI 3092 ATGCAT ATGCA^T
PflFI 1111 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2420 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2667 ATTAAT AT^TAAT
PvuII 273 CAGCTG CAG^CTG
RcaI 378 TCATGA T^CATGA
SalI 648 GTCGAC G^TCGAC
SapI 2190 GAAGAGC GCTCTTC(1/4)
StuI 2040 AGGCCT AGG^CCT
Tth111I 1111 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1176 TCTAGA T^CTAGA
XcmI 582 CCAGCCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 648 GTCGAC GT^[AC][GT]AC
1135 GTAGAC
AcsI 2487 GAATTT [GA]^AATT[CT]
3599 AAATTT
AocI 2134 CCTAAGG CC^T[ACGT]AGG
2649 CCTGAGG
ApaI 180 GGGCCC GGGCC^C
2390 GGGCCC
ApoI 2487 GAATTT [GA]^AATT[CT]
3599 AAATTT
BalI 1998 TGGCCA TGG^CCA
2358 TGGCCA
BbsI 1108 GAAGAC GAAGAC(2/6)
1152 GAAGAC
BclI 375 TGATCA T^GATCA
602 TGATCA
BpuAI 1108 GAAGAC GAAGAC(2/6)
1152 GAAGAC
BsiEI 146 CGGTCG CG[GA][CT]^CG
2643 CGGCCG
BsrDI 1676 CATTGC GCAATG(2/0)
3089 GCAATG
Bsu36I 2134 CCTAAGG CC^T[ACGT]AGG
2649 CCTGAGG
Eco81I 2134 CCTAAGG CC^T[ACGT]AGG
2649 CCTGAGG
EcoT14I 2029 CCAAGG C^C[AT][AT]GG
3482 CCAAGG
FbaI 375 TGATCA T^GATCA
602 TGATCA
HgaI 2685 GACGC GACGC(5/10)
2440 GCGTC
HincII 648 GTCGAC GT[CT]^[GA]AC
2020 GTTGAC
HindII 648 GTCGAC GT[CT]^[GA]AC
2020 GTTGAC
MluNI 1998 TGGCCA TGG^CCA
2358 TGGCCA
MscI 1998 TGGCCA TGG^CCA
2358 TGGCCA
PaeR7I 508 CTCGAG C^TCGAG
3644 CTCGAG
PstI 884 CTGCAG CTGCA^G
2321 CTGCAG
SacI 382 GAGCTC GAGCT^C
2913 GAGCTC
StyI 2029 CCAAGG C^C[AT][AT]GG
3482 CCAAGG
XhoI 508 CTCGAG C^TCGAG
3644 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
Asp700I AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI