Restriction map for
pF1KB6698
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp718I
AvrII BalI BbrPI BbvCI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DrdI
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SapI SbfI ScaI SfcI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 337 GTCGAC GT^[AC][GT]AC
AflIII 143 ACGTGT A^C[GA][CT]GT
AhdI 1249 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 13 GTGCAC G^TGCAC
AlwNI 1123 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 13 GTGCAC G^TGCAC
Asp700I 492 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1249 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 749 TGCGCA TGC^GCA
BamHI 1031 GGATCC G^GATCC
BbeI 49 GGCGCC GGCGC^C
BbsI 1047 GAAGAC GAAGAC(2/6)
BglI 29 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 248 AGATCT A^GATCT
BpuAI 1047 GAAGAC GAAGAC(2/6)
BsmFI 668 GGGAC GGGAC(10/14)
BspHI 488 TCATGA T^CATGA
BsrBI 699 GAGCGG CCGCTC(-3/-3)
BstEII 705 GGTCACC G^GT[ACGT]ACC
BstPI 705 GGTCACC G^GT[ACGT]ACC
BstXI 188 CCATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 679 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1249 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 469 GAAGAG CTCTTC(1/4)
EcoO65I 705 GGTCACC G^GT[ACGT]ACC
FspI 749 TGCGCA TGC^GCA
HincII 337 GTCGAC GT[CT]^[GA]AC
HindII 337 GTCGAC GT[CT]^[GA]AC
KasI 49 GGCGCC G^GCGCC
Ksp632I 469 GAAGAG CTCTTC(1/4)
KspI 1074 CCGCGG CCGC^GG
NarI 49 GGCGCC GG^CGCC
NspI 959 GCATGT [GA]CATG^[CT]
PpuMI 668 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 488 TCATGA T^CATGA
SacI 862 GAGCTC GAGCT^C
SacII 1074 CCGCGG CCGC^GG
SalI 337 GTCGAC G^TCGAC
SfoI 49 GGCGCC GGC^GCC
SmaI 726 CCCGGG CCC^GGG
StuI 1175 AGGCCT AGG^CCT
TfiI 137 GAATC G^A[AT]TC
XmaCI 726 CCCGGG C^CCGGG
XmaI 726 CCCGGG C^CCGGG
XmnI 492 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcyI 49 GGCGCC G[GA]^CG[CT]C
1279 GACGCC
AspHI 13 GTGCAC G[AT]GC[AT]^C
862 GAGCTC
AspI 220 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
340 GACTCGGTC
BanII 541 GAGCCC G[GA]GC[CT]^C
862 GAGCTC
BcgI 38 GCAACCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1013 GCAGCTACTTCG
BsaHI 49 GGCGCC G[GA]^CG[CT]C
1279 GACGCC
BsiHKAI 13 GTGCAC G[AT]GC[AT]^C
862 GAGCTC
BsmAI 113 GAGAC GTCTC(1/5)
386 GTCTC
BspMI 712 ACCTGC ACCTGC(4/8)
746 ACCTGC
DraII 614 AGGCCCT [GA]G^G[ACGT]CC[CT]
668 GGGACCT
EcoO109I 614 AGGCCCT [GA]G^G[ACGT]CC[CT]
668 GGGACCT
HaeII 49 GGCGCC [GA]GCGC^[CT]
846 GGCGCT
HgaI 1279 GACGC GACGC(5/10)
503 GCGTC
Hin1I 49 GGCGCC G[GA]^CG[CT]C
1279 GACGCC
NcoI 108 CCATGG C^CATGG
215 CCATGG
PflFI 220 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
340 GACTCGGTC
SexAI 680 ACCTGGT A^CC[AT]GGT
1250 ACCTGGT
Tth111I 220 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
340 GACTCGGTC
XcmI 887 CCAAGAACATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
965 CCATGAAGGAGGTGG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BanI BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiEI BsiYI BslI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DpnII
DsaI EaeI EagI EclXI Eco52I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XhoII