Restriction map for
pF1KB0498
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I ApaLI ApoI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AlwNI 133 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1174 AGCGCT AGC^GCT
ApaI 141 GGGCCC GGGCC^C
AspHI 732 GAGCTC G[AT]GC[AT]^C
BalI 221 TGGCCA TGG^CCA
BbsI 976 GAAGAC GAAGAC(2/6)
BcgI 822 GCAGCAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 804 CCGGG CC^[GC]GG
BfaI 1040 CTAG C^TAG
BglII 582 AGATCT A^GATCT
BpmI 925 CTGGAG CTGGAG(16/14)
BpuAI 976 GAAGAC GAAGAC(2/6)
BsaAI 310 TACGTG [CT]AC^GT[GA]
BseRI 601 GAGGAG GAGGAG(10/8)
BsiEI 625 CGACCG CG[GA][CT]^CG
BsiHKAI 732 GAGCTC G[AT]GC[AT]^C
BsmI 477 GAATGC GAATGC(1/-1)
BspHI 360 TCATGA T^CATGA
BsrBI 808 GAGCGG CCGCTC(-3/-3)
BsrFI 699 ACCGGC [GA]^CCGG[CT]
Cfr10I 699 ACCGGC [GA]^CCGG[CT]
DsaI 743 CCGTGG C^C[GA][CT]GG
EaeI 221 TGGCCA [CT]^GGCC[GA]
Eco47III 1174 AGCGCT AGC^GCT
EcoT14I 401 CCAAGG C^C[AT][AT]GG
HgaI 888 GACGC GACGC(5/10)
HincII 286 GTTGAC GT[CT]^[GA]AC
HindII 286 GTTGAC GT[CT]^[GA]AC
HindIII 529 AAGCTT A^AGCTT
MaeI 1040 CTAG C^TAG
MluNI 221 TGGCCA TGG^CCA
MscI 221 TGGCCA TGG^CCA
MslI 361 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 804 CCGGG CC^[GC]GG
PpuMI 1157 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 360 TCATGA T^CATGA
SacI 732 GAGCTC GAGCT^C
SapI 1215 GAAGAGC GCTCTTC(1/4)
SfaNI 1009 GATGC GCATC(5/9)
StyI 401 CCAAGG C^C[AT][AT]GG
TaqI 831 TCGA T^CGA
TfiI 1204 GAATC G^A[AT]TC
TthHB8I 831 TCGA T^CGA
XbaI 1039 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 90 GTAC GT^AC
105 GTAC
AlwI 1246 GATCC GGATC(4/5)
1150 GGATC
AocI 126 CCTGAGG CC^T[ACGT]AGG
462 CCTCAGG
AseI 214 ATTAAT AT^TAAT
960 ATTAAT
AsnI 214 ATTAAT AT^TAAT
960 ATTAAT
Asp700I 16 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
930 GAAAGACTTC
BspMI 660 GCAGGT ACCTGC(4/8)
825 GCAGGT
BstEII 663 GGTGACC G^GT[ACGT]ACC
1092 GGTGACC
BstPI 663 GGTGACC G^GT[ACGT]ACC
1092 GGTGACC
BstYI 582 AGATCT [GA]^GATC[CT]
1150 GGATCT
Bsu36I 126 CCTGAGG CC^T[ACGT]AGG
462 CCTCAGG
CfoI 868 GCGC GCG^C
1175 GCGC
Eco81I 126 CCTGAGG CC^T[ACGT]AGG
462 CCTCAGG
EcoO65I 663 GGTGACC G^GT[ACGT]ACC
1092 GGTGACC
HaeII 867 AGCGCC [GA]GCGC^[CT]
1174 AGCGCT
HhaI 868 GCGC GCG^C
1175 GCGC
HinP1I 868 GCGC G^CGC
1175 GCGC
MaeII 33 ACGT A^CGT
311 ACGT
MflI 582 AGATCT [GA]^GATC[CT]
1150 GGATCT
PshBI 214 ATTAAT AT^TAAT
960 ATTAAT
RsaI 90 GTAC GT^AC
105 GTAC
SmlI 394 CTCAAG C^T[CT][GA]AG
953 CTCAAG
TspRI 65 CAGTG CA[GC]TG[ACGT][ACGT]^
82 CAGTG
XcmI 42 CCATCCTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
339 CCAGCTGAAGGATGG
XhoII 582 AGATCT [GA]^GATC[CT]
1150 GGATCT
XmnI 16 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
930 GAAAGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI PvuII Sau3AI Sau96I
ScrFI SfcI Tru9I TseI Tsp45I
Tsp509I