Restriction map for
pF1KB0015
##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI AscI
AseI AsnI Asp718I AspI AviII
BbeI BcgI BsaBI BsiEI BsiWI
BspDI BspHI BssHII BssSI BstBI
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SspI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccI 2226 GTATAC GT^[AC][GT]AC
AflII 1351 CTTAAG C^TTAAG
AvrII 1059 CCTAGG C^CTAGG
BciVI 3235 GTATCC GTATCC(6/5)
BclI 120 TGATCA T^GATCA
BfrI 1351 CTTAAG C^TTAAG
BlnI 1059 CCTAGG C^CTAGG
BsaI 580 GAGACC GGTCTC(1/5)
Bsp1407I 4178 TGTACA T^GTACA
BsrBI 745 GAGCGG CCGCTC(-3/-3)
BsrFI 654 GCCGGT [GA]^CCGG[CT]
BsrGI 4178 TGTACA T^GTACA
Bst1107I 2226 GTATAC GTA^TAC
BstEII 1180 GGTGACC G^GT[ACGT]ACC
BstPI 1180 GGTGACC G^GT[ACGT]ACC
BstZ17I 2226 GTATAC GTA^TAC
Cfr10I 654 GCCGGT [GA]^CCGG[CT]
DraIII 3843 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3720 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1180 GGTGACC G^GT[ACGT]ACC
EcoRV 394 GATATC GAT^ATC
FbaI 120 TGATCA T^GATCA
PaeR7I 2303 CTCGAG C^TCGAG
PpuMI 1062 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 169 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1392 CCTGCAGG CCTGCA^GG
Sse8387I 1392 CCTGCAGG CCTGCA^GG
SspBI 4178 TGTACA T^GTACA
XcmI 918 CCAAGCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2303 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 1711 GACGTC GACGT^C
2110 GACGTC
AccIII 3368 TCCGGA T^CCGGA
4685 TCCGGA
Alw44I 637 GTGCAC G^TGCAC
1419 GTGCAC
AocI 1081 CCTGAGG CC^T[ACGT]AGG
2980 CCTCAGG
ApaLI 637 GTGCAC G^TGCAC
1419 GTGCAC
BamHI 1127 GGATCC G^GATCC
1855 GGATCC
BbrPI 1375 CACGTG CAC^GTG
2218 CACGTG
BbvCI 3767 CCTCAGC CCTCAGC(-5/-2)
1618 GCTGAGG
BlpI 3651 GCTCAGC GC^T[ACGT]AGC
3984 GCTGAGC
Bpu1102I 3651 GCTCAGC GC^T[ACGT]AGC
3984 GCTGAGC
BseAI 3368 TCCGGA T^CCGGA
4685 TCCGGA
BspEI 3368 TCCGGA T^CCGGA
4685 TCCGGA
BspLU11I 2230 ACATGT A^CATGT
2435 ACATGT
Bsu36I 1081 CCTGAGG CC^T[ACGT]AGG
2980 CCTCAGG
CelII 3651 GCTCAGC GC^T[ACGT]AGC
3984 GCTGAGC
Eco81I 1081 CCTGAGG CC^T[ACGT]AGG
2980 CCTCAGG
EcoRI 2949 GAATTC G^AATTC
3244 GAATTC
HgaI 2406 GACGC GACGC(5/10)
3757 GACGC
MroI 3368 TCCGGA T^CCGGA
4685 TCCGGA
NcoI 218 CCATGG C^CATGG
3569 CCATGG
PflMI 413 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1171 CCAGATGATGG
PmaCI 1375 CACGTG CAC^GTG
2218 CACGTG
PmlI 1375 CACGTG CAC^GTG
2218 CACGTG
SacI 3649 GAGCTC GAGCT^C
3883 GAGCTC
SmaI 695 CCCGGG CCC^GGG
4559 CCCGGG
Van91I 413 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1171 CCAGATGATGG
XmaCI 695 CCCGGG C^CCGGG
4559 CCCGGG
XmaI 695 CCCGGG C^CCGGG
4559 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApaI ApoI Asp700I AspEI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BglII BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV NsiI NspI PleI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI