Restriction map for
pF1KB6997
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MboI MflI
MluI MluNI MroI MscI MseI
MslI MspA1I MvnI NaeI NarI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI Sau3AI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tru9I
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AlwNI 292 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
BcnI 503 CCCGG CC^[GC]GG
BfaI 200 CTAG C^TAG
BglI 437 GCCTGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 345 CTCCAG CTGGAG(16/14)
BsmI 315 GAATGC GAATGC(1/-1)
BstF5I 561 CATCC GGATG(2/0)
DraII 21 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 389 CTCTTC CTCTTC(1/4)
EcoNI 450 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 21 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 209 CCATGG C^C[AT][AT]GG
FauI 464 CCCGC CCCGC(4/6)
FokI 561 CATCC GGATG(9/13)
HapII 504 CCGG C^CGG
HgaI 260 GACGC GACGC(5/10)
HpaII 504 CCGG C^CGG
Ksp632I 389 CTCTTC CTCTTC(1/4)
MaeI 200 CTAG C^TAG
MfeI 369 CAATTG C^AATTG
MspI 504 CCGG C^CGG
MunI 369 CAATTG C^AATTG
NciI 503 CCCGG CC^[GC]GG
NcoI 209 CCATGG C^CATGG
PflMI 603 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 268 GACTC GAGTC(4/5)
PpuMI 21 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 298 CTGCAG CTGCA^G
SapI 388 GCTCTTC GCTCTTC(1/4)
ScaI 326 AGTACT AGT^ACT
SfcI 298 CTGCAG C^T[GA][CT]AG
StyI 209 CCATGG C^C[AT][AT]GG
TfiI 620 GATTC G^A[AT]TC
Tsp509I 370 AATT ^AATT
TspRI 493 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 603 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 209 CCATGGGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 327 GTAC GT^AC
613 GTAC
AvaII 22 GGTCC G^G[AT]CC
556 GGTCC
BseRI 70 GAGGAG GAGGAG(10/8)
94 GAGGAG
BsrDI 498 CATTGC GCAATG(2/0)
551 GCAATG
BsrI 442 ACTGG ACTGG(1/-1)
603 CCAGT
BstAPI 291 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
310 GCAGAGAATGC
BstEII 180 GGTCACC G^GT[ACGT]ACC
459 GGTGACC
BstPI 180 GGTCACC G^GT[ACGT]ACC
459 GGTGACC
Cac8I 48 GCCTGC GC[ACGT]^[ACGT]GC
231 GCTCGC
DdeI 68 CTGAG C^T[ACGT]AG
270 CTCAG
DsaI 209 CCATGG C^C[GA][CT]GG
305 CCACGG
EcoO65I 180 GGTCACC G^GT[ACGT]ACC
459 GGTGACC
HinfI 268 GACTC G^A[ACGT]TC
620 GATTC
MaeII 113 ACGT A^CGT
569 ACGT
MaeIII 181 GTCAC ^GT[ACGT]AC
460 GTGAC
NlaIV 374 GGGGCC GG[ACGT]^[ACGT]CC
556 GGTCCC
RsaI 327 GTAC GT^AC
613 GTAC
SfaNI 365 GCATC GCATC(5/9)
489 GCATC
Tsp45I 181 GTCAC ^GT[GC]AC
460 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsmFI BspMI BssKI BstNI
Cfr13I EcoRII Fnu4HI HaeIII HphI
ItaI MboII MnlI MvaI MwoI
NlaIII Sau96I ScrFI TseI