##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BcnI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsiYI       BslI        BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT14I     EcoT22I     Fnu4HI      
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       ItaI        KasI        
KpnI        Ksp632I     KspI        MamI        MluI        
MroI        MspA1I      MspI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        TseI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI           37  GTAGAC               GT^[AC][GT]AC
AciI          863  GCGG                 CCGC(-3/-1)
AfaI         1248  GTAC                 GT^AC
BalI          226  TGGCCA               TGG^CCA
BclI           75  TGATCA               T^GATCA
BglII         441  AGATCT               A^GATCT
BpmI          345  CTCCAG               CTGGAG(16/14)
BsaI          250  GAGACC               GGTCTC(1/5)
BsaJI         190  CCCTGG               C^C[ACGT][ACGT]GG
BseRI          52  GAGGAG               GAGGAG(10/8)
BsmAI         250  GAGAC                GTCTC(1/5)
BsmFI         408  GGGAC                GGGAC(10/14)
BspHI         435  TCATGA               T^CATGA
BsrDI         950  GCAATG               GCAATG(2/0)
BstAPI        854  GCAAAGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         441  AGATCT               [GA]^GATC[CT]
EaeI          226  TGGCCA               [CT]^GGCC[GA]
FauI          863  GCGGG                CCCGC(4/6)
FbaI           75  TGATCA               T^GATCA
MaeII         330  ACGT                 A^CGT
MaeIII        458  GTCAC                ^GT[ACGT]AC
MfeI          421  CAATTG               C^AATTG
MflI          441  AGATCT               [GA]^GATC[CT]
MluNI         226  TGGCCA               TGG^CCA
MscI          226  TGGCCA               TGG^CCA
MunI          421  CAATTG               C^AATTG
MwoI          854  GCAAAGAATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV         186  GGAGCC               GG[ACGT]^[ACGT]CC
RcaI          435  TCATGA               T^CATGA
RsaI         1248  GTAC                 GT^AC
SacI          966  GAGCTC               GAGCT^C
SexAI         122  ACCTGGT              A^CC[AT]GGT
SmlI          633  CTCAAG               C^T[CT][GA]AG
SpeI         1168  ACTAGT               A^CTAGT
StuI          869  AGGCCT               AGG^CCT
Tsp45I        458  GTCAC                ^GT[GC]AC
XhoII         441  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AspHI         342  GTGCTC               G[AT]GC[AT]^C
              966  GAGCTC              
BanII         187  GAGCCC               G[GA]GC[CT]^C
              966  GAGCTC              
BfaI         1064  CTAG                 C^TAG
             1169  CTAG                
BsiHKAI       342  GTGCTC               G[AT]GC[AT]^C
              966  GAGCTC              
MaeI         1064  CTAG                 C^TAG
             1169  CTAG                
MboII         270  GAAGA                GAAGA(8/7)
              439  GAAGA               
SfaNI         514  GCATC                GCATC(5/9)
             1236  GCATC               
TaqI          729  TCGA                 T^CGA
              813  TCGA                
TthHB8I       729  TCGA                 T^CGA
              813  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AcsI        AluI        ApoI        BmyI        BsmI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
DdeI        DpnII       EcoRI       EcoRII      FokI        
HaeIII      HinfI       HphI        MboI        MnlI        
MseI        MslI        MvaI        NdeII       NlaIII      
PleI        Sau3AI      ScrFI       TfiI        Tru9I       
Tsp509I     TspRI