Restriction map for
pF1KB4886
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbsI BbvCI
BclI BfaI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KspI MaeI MamI MfeI MluI
MseI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tru9I Tsp509I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 431 GGTACC G^GTACC
AccIII 41 TCCGGA T^CCGGA
AlwI 51 GATCC GGATC(4/5)
AocI 320 CCTCAGG CC^T[ACGT]AGG
Asp718I 431 GGTACC G^GTACC
AspHI 778 GAGCTC G[AT]GC[AT]^C
AvaI 952 CTCGGG C^[CT]CG[GA]G
BbeI 604 GGCGCC GGCGC^C
BbrPI 744 CACGTG CAC^GTG
BcgI 681 CGACATCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 486 GGATAC GTATCC(6/5)
BpmI 10 CTGGAG CTGGAG(16/14)
BsaAI 744 CACGTG [CT]AC^GT[GA]
BsaWI 41 TCCGGA [AT]^CCGG[AT]
BseAI 41 TCCGGA T^CCGGA
BsgI 911 GTGCAG GTGCAG(16/14)
BsiEI 906 CGACCG CG[GA][CT]^CG
BsiHKAI 778 GAGCTC G[AT]GC[AT]^C
BsoBI 952 CTCGGG C^[CT]CG[GA]G
BspEI 41 TCCGGA T^CCGGA
BspMI 761 ACCTGC ACCTGC(4/8)
BsrFI 727 ACCGGC [GA]^CCGG[CT]
BstEII 957 GGTCACC G^GT[ACGT]ACC
BstPI 957 GGTCACC G^GT[ACGT]ACC
Bsu36I 320 CCTCAGG CC^T[ACGT]AGG
Cfr10I 727 ACCGGC [GA]^CCGG[CT]
EarI 754 CTCTTC CTCTTC(1/4)
Eco81I 320 CCTCAGG CC^T[ACGT]AGG
EcoO65I 957 GGTCACC G^GT[ACGT]ACC
HgaI 739 GACGC GACGC(5/10)
KasI 604 GGCGCC G^GCGCC
KpnI 431 GGTACC GGTAC^C
Ksp632I 754 CTCTTC CTCTTC(1/4)
MaeIII 958 GTCAC ^GT[ACGT]AC
MroI 41 TCCGGA T^CCGGA
NarI 604 GGCGCC GG^CGCC
NspI 70 GCATGT [GA]CATG^[CT]
PflMI 179 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 744 CACGTG CAC^GTG
PmlI 744 CACGTG CAC^GTG
PvuII 101 CAGCTG CAG^CTG
SacI 778 GAGCTC GAGCT^C
SfcI 369 CTACAG C^T[GA][CT]AG
SfoI 604 GGCGCC GGC^GCC
TaqI 758 TCGA T^CGA
Tsp45I 958 GTCAC ^GT[GC]AC
TthHB8I 758 TCGA T^CGA
Van91I 179 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 93 CCAGATCACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcyI 604 GGCGCC G[GA]^CG[CT]C
739 GACGCC
AfaI 432 GTAC GT^AC
789 GTAC
BanII 778 GAGCTC G[GA]GC[CT]^C
987 GGGCTC
BglII 170 AGATCT A^GATCT
1010 AGATCT
BsaHI 604 GGCGCC G[GA]^CG[CT]C
739 GACGCC
BseRI 288 CTCCTC GAGGAG(10/8)
781 CTCCTC
BsmAI 21 GAGAC GTCTC(1/5)
514 GAGAC
DdeI 19 CTGAG C^T[ACGT]AG
321 CTCAG
DraII 136 AGGACCC [GA]G^G[ACGT]CC[CT]
145 AGGACCC
DsaI 153 CCGTGG C^C[GA][CT]GG
983 CCACGG
EcoO109I 136 AGGACCC [GA]G^G[ACGT]CC[CT]
145 AGGACCC
HaeII 214 AGCGCC [GA]GCGC^[CT]
604 GGCGCC
Hin1I 604 GGCGCC G[GA]^CG[CT]C
739 GACGCC
MboII 701 TCTTC GAAGA(8/7)
755 TCTTC
MslI 591 CACGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
972 CATGGCTGTG
PpuMI 136 AGGACCC [GA]G^G[AT]CC[CT]
145 AGGACCC
RsaI 432 GTAC GT^AC
789 GTAC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AluI AvaII BalI
BanI BbvI BcnI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DpnII EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MboI MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SmlI TfiI TseI
TspRI XhoII