Restriction map for
pF1KB4917
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 913 GTCTAC GT^[AC][GT]AC
AflIII 677 ACGTGT A^C[GA][CT]GT
AlwNI 295 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 837 GGGCCC GGGCC^C
AvaII 957 GGACC G^G[AT]CC
BalI 92 TGGCCA TGG^CCA
BamHI 800 GGATCC G^GATCC
BanII 837 GGGCCC G[GA]GC[CT]^C
BbeI 859 GGCGCC GGCGC^C
BbsI 747 GAAGAC GAAGAC(2/6)
BglI 715 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 747 GAAGAC GAAGAC(2/6)
BsaAI 676 TACGTG [CT]AC^GT[GA]
BsaI 553 GGTCTC GGTCTC(1/5)
BseRI 658 GAGGAG GAGGAG(10/8)
BsmAI 554 GTCTC GTCTC(1/5)
Bsp1407I 134 TGTACA T^GTACA
BsrGI 134 TGTACA T^GTACA
BsrI 759 CCAGT ACTGG(1/-1)
BssHII 872 GCGCGC G^CGCGC
EcoT14I 368 CCATGG C^C[AT][AT]GG
HaeII 859 GGCGCC [GA]GCGC^[CT]
KasI 859 GGCGCC G^GCGCC
MaeII 677 ACGT A^CGT
MluNI 92 TGGCCA TGG^CCA
MscI 92 TGGCCA TGG^CCA
MseI 311 TTAA T^TAA
NaeI 691 GCCGGC GCC^GGC
NarI 859 GGCGCC GG^CGCC
NcoI 368 CCATGG C^CATGG
NgoMIV 691 GCCGGC G^CCGGC
PpuMI 956 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 219 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 859 GGCGCC GGC^GCC
SspBI 134 TGTACA T^GTACA
StyI 368 CCATGG C^C[AT][AT]GG
TaqI 86 TCGA T^CGA
Tru9I 311 TTAA T^TAA
TspRI 476 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 86 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaI 232 CCCGAG C^[CT]CG[GA]G
568 CTCGGG
BmyI 464 GTGCCC G[AGT]GC[ACT]^C
837 GGGCCC
BpmI 485 CTCCAG CTGGAG(16/14)
757 CTCCAG
BsoBI 232 CCCGAG C^[CT]CG[GA]G
568 CTCGGG
Bsp1286I 464 GTGCCC G[AGT]GC[ACT]^C
837 GGGCCC
BsrBI 171 CCGCTC CCGCTC(-3/-3)
616 CCGCTC
BsrDI 408 CATTGC GCAATG(2/0)
546 CATTGC
BsrFI 550 GCCGGT [GA]^CCGG[CT]
691 GCCGGC
BssSI 138 CACGAG CACGAG(-5/-1)
832 CACGAG
BstF5I 396 GGATG GGATG(2/0)
588 GGATG
Cfr10I 550 GCCGGT [GA]^CCGG[CT]
691 GCCGGC
FokI 396 GGATG GGATG(9/13)
588 GGATG
HincII 727 GTCAAC GT[CT]^[GA]AC
778 GTCAAC
HindII 727 GTCAAC GT[CT]^[GA]AC
778 GTCAAC
HinfI 566 GACTC G^A[ACGT]TC
934 GAGTC
NspI 143 GCATGT [GA]CATG^[CT]
191 GCATGT
PleI 566 GACTC GAGTC(4/5)
934 GAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI BanI BbvI BcnI BsaHI
BsaJI BsiYI BslI BsmFI BspMI
BssKI BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I XhoII