Restriction map for
pF1KB9279
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
BmyI Bpu1102I BpuAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRII EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI MamI MfeI MluI
MroI MseI MunI MvaI NaeI
NarI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 457 CGCG CG^CG
AflIII 70 ACATGT A^C[GA][CT]GT
AlwI 340 GATCC GGATC(4/5)
AlwNI 563 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 484 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 187 GGACC G^G[AT]CC
BalI 896 TGGCCA TGG^CCA
BcnI 184 CCCGG CC^[GC]GG
BglII 784 AGATCT A^GATCT
BpmI 155 CTGGAG CTGGAG(16/14)
BsaAI 761 TACGTG [CT]AC^GT[GA]
BsaI 833 GGTCTC GGTCTC(1/5)
BsmFI 11 GGGAC GGGAC(10/14)
BspLU11I 70 ACATGT A^CATGT
BspMI 382 GCAGGT ACCTGC(4/8)
BssKI 184 CCCGG ^CC[ACGT]GG
BstF5I 40 GGATG GGATG(2/0)
BstUI 457 CGCG CG^CG
BstYI 784 AGATCT [GA]^GATC[CT]
Cac8I 690 GCTAGC GC[ACGT]^[ACGT]GC
EaeI 896 TGGCCA [CT]^GGCC[GA]
EcoT14I 149 CCATGG C^C[AT][AT]GG
FauI 458 GCGGG CCCGC(4/6)
FokI 40 GGATG GGATG(9/13)
HgaI 117 GACGC GACGC(5/10)
HincII 166 GTCAAC GT[CT]^[GA]AC
HindII 166 GTCAAC GT[CT]^[GA]AC
HphI 207 GGTGA GGTGA(8/7)
KspI 456 CCGCGG CCGC^GG
MaeII 762 ACGT A^CGT
MflI 784 AGATCT [GA]^GATC[CT]
MluNI 896 TGGCCA TGG^CCA
MscI 896 TGGCCA TGG^CCA
MvnI 457 CGCG CG^CG
NciI 184 CCCGG CC^[GC]GG
NcoI 149 CCATGG C^CATGG
NheI 690 GCTAGC G^CTAGC
PshAI 98 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 100 CTGCAG CTGCA^G
SacII 456 CCGCGG CCGC^GG
ScrFI 184 CCCGG CC^[ACGT]GG
SmlI 353 CTCAAG C^T[CT][GA]AG
StyI 149 CCATGG C^C[AT][AT]GG
Tsp45I 115 GTGAC ^GT[GC]AC
XcmI 899 CCACCTATGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 784 AGATCT [GA]^GATC[CT]
XmnI 484 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 456 CCGC CCGC(-3/-1)
458 GCGG
AfaI 50 GTAC GT^AC
358 GTAC
BsaJI 149 CCATGG C^C[ACGT][ACGT]GG
456 CCGCGG
BsrDI 196 CATTGC GCAATG(2/0)
376 GCAATG
BstXI 257 CCAATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
887 CCATTATGATGG
DraII 225 AGGGCCT [GA]G^G[ACGT]CC[CT]
252 AGGCCCC
DsaI 149 CCATGG C^C[GA][CT]GG
456 CCGCGG
EcoO109I 225 AGGGCCT [GA]G^G[ACGT]CC[CT]
252 AGGCCCC
HapII 35 CCGG C^CGG
185 CCGG
HpaII 35 CCGG C^CGG
185 CCGG
MaeIII 115 GTGAC ^GT[ACGT]AC
435 GTTAC
MslI 15 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
888 CATTATGATG
MspI 35 CCGG C^CGG
185 CCGG
NdeI 361 CATATG CA^TATG
548 CATATG
NspI 70 ACATGT [GA]CATG^[CT]
536 GCATGT
PvuII 139 CAGCTG CAG^CTG
371 CAGCTG
RsaI 50 GTAC GT^AC
358 GTAC
SfcI 100 CTGCAG C^T[GA][CT]AG
725 CTATAG
TfiI 346 GATTC G^A[AT]TC
873 GATTC
Tsp509I 259 AATT ^AATT
908 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI BbvI BfaI BsmAI BsrI
Cfr13I DpnII EarI Fnu4HI HaeIII
HinfI ItaI Ksp632I MaeI MboI
MboII MnlI MspA1I MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
SfaNI TseI TspRI