################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI MamI MfeI MluI MroI MseI MunI MvaI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 457 CGCG CG^CG AflIII 70 ACATGT A^C[GA][CT]GT AlwI 340 GATCC GGATC(4/5) AlwNI 563 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 484 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 187 GGACC G^G[AT]CC BalI 896 TGGCCA TGG^CCA BcnI 184 CCCGG CC^[GC]GG BglII 784 AGATCT A^GATCT BpmI 155 CTGGAG CTGGAG(16/14) BsaAI 761 TACGTG [CT]AC^GT[GA] BsaI 833 GGTCTC GGTCTC(1/5) BsmFI 11 GGGAC GGGAC(10/14) BspLU11I 70 ACATGT A^CATGT BspMI 382 GCAGGT ACCTGC(4/8) BssKI 184 CCCGG ^CC[ACGT]GG BstF5I 40 GGATG GGATG(2/0) BstUI 457 CGCG CG^CG BstYI 784 AGATCT [GA]^GATC[CT] Cac8I 690 GCTAGC GC[ACGT]^[ACGT]GC EaeI 896 TGGCCA [CT]^GGCC[GA] EcoT14I 149 CCATGG C^C[AT][AT]GG FauI 458 GCGGG CCCGC(4/6) FokI 40 GGATG GGATG(9/13) HgaI 117 GACGC GACGC(5/10) HincII 166 GTCAAC GT[CT]^[GA]AC HindII 166 GTCAAC GT[CT]^[GA]AC HphI 207 GGTGA GGTGA(8/7) KspI 456 CCGCGG CCGC^GG MaeII 762 ACGT A^CGT MflI 784 AGATCT [GA]^GATC[CT] MluNI 896 TGGCCA TGG^CCA MscI 896 TGGCCA TGG^CCA MvnI 457 CGCG CG^CG NciI 184 CCCGG CC^[GC]GG NcoI 149 CCATGG C^CATGG NheI 690 GCTAGC G^CTAGC PshAI 98 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 100 CTGCAG CTGCA^G SacII 456 CCGCGG CCGC^GG ScrFI 184 CCCGG CC^[ACGT]GG SmlI 353 CTCAAG C^T[CT][GA]AG StyI 149 CCATGG C^C[AT][AT]GG Tsp45I 115 GTGAC ^GT[GC]AC XcmI 899 CCACCTATGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 784 AGATCT [GA]^GATC[CT] XmnI 484 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AciI 456 CCGC CCGC(-3/-1) 458 GCGG AfaI 50 GTAC GT^AC 358 GTAC BsaJI 149 CCATGG C^C[ACGT][ACGT]GG 456 CCGCGG BsrDI 196 CATTGC GCAATG(2/0) 376 GCAATG BstXI 257 CCAATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 887 CCATTATGATGG DraII 225 AGGGCCT [GA]G^G[ACGT]CC[CT] 252 AGGCCCC DsaI 149 CCATGG C^C[GA][CT]GG 456 CCGCGG EcoO109I 225 AGGGCCT [GA]G^G[ACGT]CC[CT] 252 AGGCCCC HapII 35 CCGG C^CGG 185 CCGG HpaII 35 CCGG C^CGG 185 CCGG MaeIII 115 GTGAC ^GT[ACGT]AC 435 GTTAC MslI 15 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 888 CATTATGATG MspI 35 CCGG C^CGG 185 CCGG NdeI 361 CATATG CA^TATG 548 CATATG NspI 70 ACATGT [GA]CATG^[CT] 536 GCATGT PvuII 139 CAGCTG CAG^CTG 371 CAGCTG RsaI 50 GTAC GT^AC 358 GTAC SfcI 100 CTGCAG C^T[GA][CT]AG 725 CTATAG TfiI 346 GATTC G^A[AT]TC 873 GATTC Tsp509I 259 AATT ^AATT 908 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AluI BbvI BfaI BsmAI BsrI Cfr13I DpnII EarI Fnu4HI HaeIII HinfI ItaI Ksp632I MaeI MboI MboII MnlI MspA1I MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I SfaNI TseI TspRI