Restriction map for pF1KB9279
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DdeI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HindIII     HpaI        
KasI        KpnI        MamI        MfeI        MluI        
MroI        MseI        MunI        MvaI        NaeI        
NarI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tru9I       Tth111I     TthHB8I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII         457  CGCG                 CG^CG
AflIII         70  ACATGT               A^C[GA][CT]GT
AlwI          340  GATCC                GGATC(4/5)
AlwNI         563  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       484  GAATGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         187  GGACC                G^G[AT]CC
BalI          896  TGGCCA               TGG^CCA
BcnI          184  CCCGG                CC^[GC]GG
BglII         784  AGATCT               A^GATCT
BpmI          155  CTGGAG               CTGGAG(16/14)
BsaAI         761  TACGTG               [CT]AC^GT[GA]
BsaI          833  GGTCTC               GGTCTC(1/5)
BsmFI          11  GGGAC                GGGAC(10/14)
BspLU11I       70  ACATGT               A^CATGT
BspMI         382  GCAGGT               ACCTGC(4/8)
BssKI         184  CCCGG                ^CC[ACGT]GG
BstF5I         40  GGATG                GGATG(2/0)
BstUI         457  CGCG                 CG^CG
BstYI         784  AGATCT               [GA]^GATC[CT]
Cac8I         690  GCTAGC               GC[ACGT]^[ACGT]GC
EaeI          896  TGGCCA               [CT]^GGCC[GA]
EcoT14I       149  CCATGG               C^C[AT][AT]GG
FauI          458  GCGGG                CCCGC(4/6)
FokI           40  GGATG                GGATG(9/13)
HgaI          117  GACGC                GACGC(5/10)
HincII        166  GTCAAC               GT[CT]^[GA]AC
HindII        166  GTCAAC               GT[CT]^[GA]AC
HphI          207  GGTGA                GGTGA(8/7)
KspI          456  CCGCGG               CCGC^GG
MaeII         762  ACGT                 A^CGT
MflI          784  AGATCT               [GA]^GATC[CT]
MluNI         896  TGGCCA               TGG^CCA
MscI          896  TGGCCA               TGG^CCA
MvnI          457  CGCG                 CG^CG
NciI          184  CCCGG                CC^[GC]GG
NcoI          149  CCATGG               C^CATGG
NheI          690  GCTAGC               G^CTAGC
PshAI          98  GACTGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          100  CTGCAG               CTGCA^G
SacII         456  CCGCGG               CCGC^GG
ScrFI         184  CCCGG                CC^[ACGT]GG
SmlI          353  CTCAAG               C^T[CT][GA]AG
StyI          149  CCATGG               C^C[AT][AT]GG
Tsp45I        115  GTGAC                ^GT[GC]AC
XcmI          899  CCACCTATGAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         784  AGATCT               [GA]^GATC[CT]
XmnI          484  GAATGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AciI          456  CCGC                 CCGC(-3/-1)
              458  GCGG                
AfaI           50  GTAC                 GT^AC
              358  GTAC                
BsaJI         149  CCATGG               C^C[ACGT][ACGT]GG
              456  CCGCGG              
BsrDI         196  CATTGC               GCAATG(2/0)
              376  GCAATG              
BstXI         257  CCAATTTAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              887  CCATTATGATGG        
DraII         225  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              252  AGGCCCC             
DsaI          149  CCATGG               C^C[GA][CT]GG
              456  CCGCGG              
EcoO109I      225  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              252  AGGCCCC             
HapII          35  CCGG                 C^CGG
              185  CCGG                
HpaII          35  CCGG                 C^CGG
              185  CCGG                
MaeIII        115  GTGAC                ^GT[ACGT]AC
              435  GTTAC               
MslI           15  CACGCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              888  CATTATGATG          
MspI           35  CCGG                 C^CGG
              185  CCGG                
NdeI          361  CATATG               CA^TATG
              548  CATATG              
NspI           70  ACATGT               [GA]CATG^[CT]
              536  GCATGT              
PvuII         139  CAGCTG               CAG^CTG
              371  CAGCTG              
RsaI           50  GTAC                 GT^AC
              358  GTAC                
SfcI          100  CTGCAG               C^T[GA][CT]AG
              725  CTATAG              
TfiI          346  GATTC                G^A[AT]TC
              873  GATTC               
Tsp509I       259  AATT                 ^AATT
              908  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AluI        BbvI        BfaI        BsmAI       BsrI        
Cfr13I      DpnII       EarI        Fnu4HI      HaeIII      
HinfI       ItaI        Ksp632I     MaeI        MboI        
MboII       MnlI        MspA1I      MwoI        NdeII       
NlaIII      NlaIV       PleI        Sau3AI      Sau96I      
SfaNI       TseI        TspRI       
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