Restriction map for
pF1KE0758
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MunI MvnI MwoI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI Tsp45I Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AciI 664 CCGC CCGC(-3/-1)
AfaI 986 GTAC GT^AC
AflIII 750 ACATGT A^C[GA][CT]GT
AlwI 828 GATCC GGATC(4/5)
AocI 909 CCTTAGG CC^T[ACGT]AGG
AseI 388 ATTAAT AT^TAAT
AsnI 388 ATTAAT AT^TAAT
AvaII 415 GGACC G^G[AT]CC
BbsI 425 GTCTTC GAAGAC(2/6)
BbvI 233 GCAGC GCAGC(8/12)
BfaI 277 CTAG C^TAG
BpuAI 425 GTCTTC GAAGAC(2/6)
BsaI 24 GGTCTC GGTCTC(1/5)
BsmAI 25 GTCTC GTCTC(1/5)
BsmI 1120 GAATGC GAATGC(1/-1)
BspLU11I 750 ACATGT A^CATGT
BsrDI 1286 GCAATG GCAATG(2/0)
BstXI 813 CCAGAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 909 CCTTAGG CC^T[ACGT]AGG
Cfr13I 415 GGACC G^G[ACGT]CC
DrdI 416 GACCAATAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 88 CCATGG C^C[GA][CT]GG
EarI 135 GAAGAG CTCTTC(1/4)
Eco81I 909 CCTTAGG CC^T[ACGT]AGG
Fnu4HI 233 GCAGC GC^[ACGT]GC
ItaI 233 GCAGC GC^[ACGT]GC
Ksp632I 135 GAAGAG CTCTTC(1/4)
MaeI 277 CTAG C^TAG
MnlI 356 GAGG CCTC(7/6)
MspA1I 1230 CAGCTG C[AC]G^C[GT]G
NcoI 88 CCATGG C^CATGG
NlaIV 17 GGAACC GG[ACGT]^[ACGT]CC
PflMI 297 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 388 ATTAAT AT^TAAT
PvuII 1230 CAGCTG CAG^CTG
RsaI 986 GTAC GT^AC
SapI 135 GAAGAGC GCTCTTC(1/4)
Sau96I 415 GGACC G^G[ACGT]CC
SexAI 20 ACCTGGT A^CC[AT]GGT
SfaNI 666 GCATC GCATC(5/9)
SfcI 1233 CTGTAG C^T[GA][CT]AG
SspI 538 AATATT AAT^ATT
TseI 233 GCAGC G^C[AT]GC
Van91I 297 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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BcnI 599 CCGGG CC^[GC]GG
767 CCGGG
BsiYI 204 CCTAATGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
297 CCAAAGAATGG
BslI 204 CCTAATGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
297 CCAAAGAATGG
DpnII 442 GATC ^GATC
828 GATC
EcoT14I 88 CCATGG C^C[AT][AT]GG
248 CCTTGG
HaeIII 202 GGCC GG^CC
358 GGCC
HapII 599 CCGG C^CGG
767 CCGG
HpaII 599 CCGG C^CGG
767 CCGG
HphI 685 GGTGA GGTGA(8/7)
570 TCACC
MboI 442 GATC ^GATC
828 GATC
MspI 599 CCGG C^CGG
767 CCGG
NciI 599 CCGGG CC^[GC]GG
767 CCGGG
NdeII 442 GATC ^GATC
828 GATC
Sau3AI 442 GATC ^GATC
828 GATC
StyI 88 CCATGG C^C[AT][AT]GG
248 CCTTGG
TfiI 257 GAATC G^A[AT]TC
1153 GATTC
TspRI 29 CAGTG CA[GC]TG[ACGT][ACGT]^
1255 CACTG
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AcsI AluI ApoI Asp700I BpmI
BsaJI BsrI BssKI BstF5I BstNI
DdeI EcoRI EcoRII FokI HinfI
MboII MseI MslI MvaI NlaIII
NspI PleI ScrFI Tru9I Tsp509I
XmnI