Restriction map for
pF1KE1014
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BamHI
BbeI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BpmI BsaBI BsaHI BsaI BseRI
BsiWI BsmBI BsmI BsoBI BspDI
BspHI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraII DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MflI MluI MunI NarI
NciI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SpeI Sse8387I SspI StuI Tsp509I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 759 CGCG CG^CG
AccIII 879 TCCGGA T^CCGGA
BanI 862 GGTGCC G^G[CT][GA]CC
BbsI 565 GTCTTC GAAGAC(2/6)
BbvCI 606 GCTGAGG CCTCAGC(-5/-2)
BlpI 102 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 102 GCTCAGC GC^T[ACGT]AGC
BpuAI 565 GTCTTC GAAGAC(2/6)
BseAI 879 TCCGGA T^CCGGA
BsgI 828 GTGCAG GTGCAG(16/14)
BsiEI 263 CGGCCG CG[GA][CT]^CG
BsmFI 833 GGGAC GGGAC(10/14)
Bsp1407I 472 TGTACA T^GTACA
BspEI 879 TCCGGA T^CCGGA
BspMI 859 GCAGGT ACCTGC(4/8)
BsrGI 472 TGTACA T^GTACA
BstEII 186 GGTGACC G^GT[ACGT]ACC
BstPI 186 GGTGACC G^GT[ACGT]ACC
BstUI 759 CGCG CG^CG
CelII 102 GCTCAGC GC^T[ACGT]AGC
DraI 138 TTTAAA TTT^AAA
DsaI 182 CCGTGG C^C[GA][CT]GG
EagI 263 CGGCCG C^GGCCG
EclXI 263 CGGCCG C^GGCCG
Eco52I 263 CGGCCG C^GGCCG
EcoO65I 186 GGTGACC G^GT[ACGT]ACC
EcoT14I 215 CCTTGG C^C[AT][AT]GG
HaeII 430 AGCGCC [GA]GCGC^[CT]
MroI 879 TCCGGA T^CCGGA
MseI 139 TTAA T^TAA
MslI 850 CACAGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 105 CAGCTG C[AC]G^C[GT]G
MvnI 759 CGCG CG^CG
NaeI 265 GCCGGC GCC^GGC
NgoMIV 265 GCCGGC G^CCGGC
PvuII 105 CAGCTG CAG^CTG
SnaBI 151 TACGTA TAC^GTA
SphI 269 GCATGC GCATG^C
SspBI 472 TGTACA T^GTACA
StyI 215 CCTTGG C^C[AT][AT]GG
SwaI 137 ATTTAAAT ATTT^AAAT
Tru9I 139 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 469 ACGTGT A^C[GA][CT]GT
503 ACGTGT
AlwI 512 GGATC GGATC(4/5)
611 GGATC
AlwNI 544 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
670 CAGAACCTG
AspI 559 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
592 GACGTGGTC
BalI 812 TGGCCA TGG^CCA
897 TGGCCA
BbrPI 468 CACGTG CAC^GTG
765 CACGTG
BsaWI 239 ACCGGA [AT]^CCGG[AT]
879 TCCGGA
BsmAI 716 GAGAC GTCTC(1/5)
883 GAGAC
EarI 25 CTCTTC CTCTTC(1/4)
34 CTCTTC
FauI 276 GCGGG CCCGC(4/6)
579 GCGGG
Ksp632I 25 CTCTTC CTCTTC(1/4)
34 CTCTTC
MluNI 812 TGGCCA TGG^CCA
897 TGGCCA
MscI 812 TGGCCA TGG^CCA
897 TGGCCA
PflFI 559 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
592 GACGTGGTC
PflMI 351 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
815 CCATAGGCTGG
PleI 172 GAGTC GAGTC(4/5)
876 GAGTC
PmaCI 468 CACGTG CAC^GTG
765 CACGTG
PmlI 468 CACGTG CAC^GTG
765 CACGTG
PstI 550 CTGCAG CTGCA^G
664 CTGCAG
SapI 24 GCTCTTC GCTCTTC(1/4)
33 GCTCTTC
SfaNI 839 GATGC GCATC(5/9)
773 GCATC
SfcI 550 CTGCAG C^T[GA][CT]AG
664 CTGCAG
TaqI 48 TCGA T^CGA
175 TCGA
TfiI 362 GATTC G^A[AT]TC
732 GAATC
Tth111I 559 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
592 GACGTGGTC
TthHB8I 48 TCGA T^CGA
175 TCGA
Van91I 351 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
815 CCATAGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BmyI BsaAI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI RsaI
SacI Sau3AI Sau96I ScrFI TseI
Tsp45I TspRI