Restriction map for
pF1KE0754
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I AlwI AocI
ApaLI AscI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BseAI
BsgI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MspA1I MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI Tsp45I
TspRI Tth111I TthHB8I Van91I XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AflIII 583 ACATGT A^C[GA][CT]GT
AgeI 157 ACCGGT A^CCGGT
AlwNI 1013 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 370 AGCGCT AGC^GCT
ApaI 791 GGGCCC GGGCC^C
AseI 730 ATTAAT AT^TAAT
AsnI 730 ATTAAT AT^TAAT
Asp700I 397 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 997 GTGCTC G[AT]GC[AT]^C
BanI 154 GGCACC G^G[CT][GA]CC
BclI 717 TGATCA T^GATCA
BpmI 187 CTGGAG CTGGAG(16/14)
BsaAI 211 TACGTG [CT]AC^GT[GA]
BsaI 838 GAGACC GGTCTC(1/5)
BsaJI 5 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 157 ACCGGT [AT]^CCGG[AT]
BsiEI 159 CGGTCG CG[GA][CT]^CG
BsiHKAI 997 GTGCTC G[AT]GC[AT]^C
BspLU11I 583 ACATGT A^CATGT
BspMI 303 ACCTGC ACCTGC(4/8)
BsrFI 157 ACCGGT [GA]^CCGG[CT]
BsrI 116 CCAGT ACTGG(1/-1)
BstXI 680 CCAAAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 371 GCGC GCG^C
Cfr10I 157 ACCGGT [GA]^CCGG[CT]
DraII 790 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 487 GACACAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 941 TGGCCG [CT]^GGCC[GA]
Eco47III 370 AGCGCT AGC^GCT
EcoO109I 790 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 5 CCTTGG C^C[AT][AT]GG
FauI 77 GCGGG CCCGC(4/6)
FbaI 717 TGATCA T^GATCA
HaeII 370 AGCGCT [GA]GCGC^[CT]
HhaI 371 GCGC GCG^C
HinP1I 371 GCGC G^CGC
HindIII 1197 AAGCTT A^AGCTT
HphI 405 TCACC GGTGA(8/7)
MaeIII 580 GTTAC ^GT[ACGT]AC
MslI 939 CATGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 583 ACATGT [GA]CATG^[CT]
PinAI 157 ACCGGT A^CCGGT
PshBI 730 ATTAAT AT^TAAT
PstI 1178 CTGCAG CTGCA^G
SexAI 441 ACCTGGT A^CC[AT]GGT
SspI 727 AATATT AAT^ATT
StyI 5 CCTTGG C^C[AT][AT]GG
XbaI 847 TCTAGA T^CTAGA
XmnI 397 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 76 CGCG CG^CG
130 CGCG
AcyI 168 GGCGTC G[GA]^CG[CT]C
1262 GACGCC
BanII 190 GAGCCC G[GA]GC[CT]^C
791 GGGCCC
BbsI 91 GAAGAC GAAGAC(2/6)
484 GAAGAC
BbvI 691 GCAGC GCAGC(8/12)
763 GCTGC
BfaI 376 CTAG C^TAG
848 CTAG
BpuAI 91 GAAGAC GAAGAC(2/6)
484 GAAGAC
BsaHI 168 GGCGTC G[GA]^CG[CT]C
1262 GACGCC
BseRI 256 GAGGAG GAGGAG(10/8)
754 GAGGAG
BsmFI 1155 GGGAC GGGAC(10/14)
171 GTCCC
BssKI 45 CCAGG ^CC[ACGT]GG
442 CCTGG
BstNI 45 CCAGG CC^[AT]GG
442 CCTGG
BstUI 76 CGCG CG^CG
130 CGCG
Cac8I 165 GCTGGC GC[ACGT]^[ACGT]GC
771 GCAAGC
Cfr13I 791 GGGCC G^G[ACGT]CC
792 GGCCC
DraI 613 TTTAAA TTT^AAA
1102 TTTAAA
EcoRII 45 CCAGG ^CC[AT]GG
442 CCTGG
HapII 88 CCGG C^CGG
158 CCGG
HgaI 1262 GACGC GACGC(5/10)
169 GCGTC
Hin1I 168 GGCGTC G[GA]^CG[CT]C
1262 GACGCC
HpaII 88 CCGG C^CGG
158 CCGG
MaeI 376 CTAG C^TAG
848 CTAG
MaeII 212 ACGT A^CGT
702 ACGT
MspI 88 CCGG C^CGG
158 CCGG
MvaI 45 CCAGG CC^[AT]GG
442 CCTGG
MvnI 76 CGCG CG^CG
130 CGCG
PleI 81 GAGTC GAGTC(4/5)
1073 GAGTC
Sau96I 791 GGGCC G^G[ACGT]CC
792 GGCCC
ScrFI 45 CCAGG CC^[ACGT]GG
442 CCTGG
SfaNI 832 GCATC GCATC(5/9)
974 GCATC
SfcI 96 CTACAG C^T[GA][CT]AG
1178 CTGCAG
TseI 691 GCAGC G^C[AT]GC
763 GCTGC
XcmI 141 CCATTCCAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
795 CCAGATTGCAGATGG
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BmyI BsiYI BslI BsmAI Bsp1286I
BstF5I DdeI DpnII EarI Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MboI MboII MnlI MseI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
TfiI Tru9I Tsp509I