Restriction map for
pF1KB3444
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseRI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstNI BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MflI MluI
MseI MvaI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 41 TCCGGA T^CCGGA
AciI 158 CCGC CCGC(-3/-1)
AcsI 37 AAATTC [GA]^AATT[CT]
AfaI 226 GTAC GT^AC
AlwI 131 GGATC GGATC(4/5)
ApoI 37 AAATTC [GA]^AATT[CT]
AspHI 211 GAGCTC G[AT]GC[AT]^C
BalI 185 TGGCCA TGG^CCA
BanII 211 GAGCTC G[GA]GC[CT]^C
BbsI 56 GTCTTC GAAGAC(2/6)
BcnI 389 CCCGG CC^[GC]GG
BmyI 211 GAGCTC G[AGT]GC[ACT]^C
BpuAI 56 GTCTTC GAAGAC(2/6)
BseAI 41 TCCGGA T^CCGGA
BsgI 374 GTGCAG GTGCAG(16/14)
BsiHKAI 211 GAGCTC G[AT]GC[AT]^C
BsmFI 138 GGGAC GGGAC(10/14)
Bsp1286I 211 GAGCTC G[AGT]GC[ACT]^C
BspEI 41 TCCGGA T^CCGGA
BsrDI 414 CATTGC GCAATG(2/0)
BsrI 358 CCAGT ACTGG(1/-1)
BssKI 389 CCCGG ^CC[ACGT]GG
BstAPI 397 GCATCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 406 GCTGGC GC[ACGT]^[ACGT]GC
DdeI 214 CTCAG C^T[ACGT]AG
DpnII 132 GATC ^GATC
DraII 22 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 185 TGGCCA [CT]^GGCC[GA]
EcoO109I 22 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 50 CCTTGG C^C[AT][AT]GG
FauI 157 CCCGC CCCGC(4/6)
HinfI 308 GAGTC G^A[ACGT]TC
MaeII 15 ACGT A^CGT
MaeIII 173 GTGAC ^GT[ACGT]AC
MboI 132 GATC ^GATC
MfeI 78 CAATTG C^AATTG
MluNI 185 TGGCCA TGG^CCA
MroI 41 TCCGGA T^CCGGA
MscI 185 TGGCCA TGG^CCA
MspA1I 158 CCGCTG C[AC]G^C[GT]G
MunI 78 CAATTG C^AATTG
MwoI 397 GCATCCAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 389 CCCGG CC^[GC]GG
NdeII 132 GATC ^GATC
NlaIII 177 CATG CATG^
PleI 308 GAGTC GAGTC(4/5)
PpuMI 22 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 226 GTAC GT^AC
SacI 211 GAGCTC GAGCT^C
Sau3AI 132 GATC ^GATC
ScrFI 389 CCCGG CC^[ACGT]GG
SfaNI 397 GCATC GCATC(5/9)
StyI 50 CCTTGG C^C[AT][AT]GG
Tsp45I 173 GTGAC ^GT[GC]AC
TspRI 236 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 20 enzymes) #
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AluI 69 AGCT AG^CT
212 AGCT
AvaII 23 GGTCC G^G[AT]CC
355 GGACC
BbvI 410 GCAGC GCAGC(8/12)
160 GCTGC
BsaJI 50 CCTTGG C^C[ACGT][ACGT]GG
388 CCCCGG
BsaWI 41 TCCGGA [AT]^CCGG[AT]
60 TCCGGT
BsiYI 122 CCGTGAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
157 CCCGCTGCTGG
BslI 122 CCGTGAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
157 CCCGCTGCTGG
BstF5I 229 CATCC GGATG(2/0)
398 CATCC
Fnu4HI 160 GCTGC GC^[ACGT]GC
410 GCAGC
FokI 229 CATCC GGATG(9/13)
398 CATCC
HaeIII 186 GGCC GG^CC
386 GGCC
HphI 172 GGTGA GGTGA(8/7)
180 GGTGA
ItaI 160 GCTGC GC^[ACGT]GC
410 GCAGC
MboII 295 GAAGA GAAGA(8/7)
57 TCTTC
MnlI 26 CCTC CCTC(7/6)
11 GAGG
MslI 117 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
177 CATGGTGATG
TaqI 4 TCGA T^CGA
246 TCGA
TseI 160 GCTGC G^C[AT]GC
410 GCAGC
Tsp509I 38 AATT ^AATT
79 AATT
TthHB8I 4 TCGA T^CGA
246 TCGA
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# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
Cfr13I HapII HpaII MspI NlaIV
Sau96I