Restriction map for
pF1KE0750
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BbvI BcgI BclI BcnI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseAI BsiEI BsiWI
BsiYI BslI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraII
DraIII DrdI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI ItaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MspA1I MunI MvnI MwoI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI TaqI
TseI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflII 418 CTTAAG C^TTAAG
AflIII 999 ACATGT A^C[GA][CT]GT
AgeI 95 ACCGGT A^CCGGT
AhdI 91 GACCACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 493 GTGCAC G^TGCAC
AlwI 884 GATCC GGATC(4/5)
ApaLI 493 GTGCAC G^TGCAC
AspEI 91 GACCACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 90 GGACC G^G[AT]CC
BalI 1047 TGGCCA TGG^CCA
BanII 434 GAGCTC G[GA]GC[CT]^C
BciVI 338 GGATAC GTATCC(6/5)
BfrI 418 CTTAAG C^TTAAG
BglII 1147 AGATCT A^GATCT
BsaWI 95 ACCGGT [AT]^CCGG[AT]
BseRI 157 GAGGAG GAGGAG(10/8)
BsgI 827 CTGCAC GTGCAG(16/14)
BsmFI 18 GTCCC GGGAC(10/14)
BspLU11I 999 ACATGT A^CATGT
BsrFI 95 ACCGGT [GA]^CCGG[CT]
BstXI 154 CCAGAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 649 GCAAGC GC[ACGT]^[ACGT]GC
Cfr10I 95 ACCGGT [GA]^CCGG[CT]
DsaI 21 CCATGG C^C[GA][CT]GG
EaeI 1047 TGGCCA [CT]^GGCC[GA]
Eam1105I 91 GACCACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1175 GAATTC G^AATTC
EcoT14I 21 CCATGG C^C[AT][AT]GG
HapII 96 CCGG C^CGG
HpaII 96 CCGG C^CGG
MaeII 124 ACGT A^CGT
MaeIII 121 GTGAC ^GT[ACGT]AC
MluNI 1047 TGGCCA TGG^CCA
MscI 1047 TGGCCA TGG^CCA
MspI 96 CCGG C^CGG
NcoI 21 CCATGG C^CATGG
NlaIV 39 GGAACC GG[ACGT]^[ACGT]CC
PinAI 95 ACCGGT A^CCGGT
SacI 434 GAGCTC GAGCT^C
SmlI 418 CTTAAG C^T[CT][GA]AG
SspI 327 AATATT AAT^ATT
StuI 567 AGGCCT AGG^CCT
StyI 21 CCATGG C^C[AT][AT]GG
Tsp45I 121 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 953 GTAC GT^AC
1065 GTAC
AluI 358 AGCT AG^CT
435 AGCT
AspHI 434 GAGCTC G[AT]GC[AT]^C
493 GTGCAC
BfaI 281 CTAG C^TAG
977 CTAG
BmyI 434 GAGCTC G[AGT]GC[ACT]^C
493 GTGCAC
BsiHKAI 434 GAGCTC G[AT]GC[AT]^C
493 GTGCAC
Bsp1286I 434 GAGCTC G[AGT]GC[ACT]^C
493 GTGCAC
BstF5I 902 CATCC GGATG(2/0)
367 GGATG
BstYI 883 AGATCC [GA]^GATC[CT]
1147 AGATCT
Cfr13I 90 GGACC G^G[ACGT]CC
1071 GGGCC
FokI 902 CATCC GGATG(9/13)
367 GGATG
MaeI 281 CTAG C^TAG
977 CTAG
MflI 883 AGATCC [GA]^GATC[CT]
1147 AGATCT
MslI 486 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
944 CACACAAATG
NspI 831 ACATGC [GA]CATG^[CT]
999 ACATGT
RsaI 953 GTAC GT^AC
1065 GTAC
Sau96I 90 GGACC G^G[ACGT]CC
1071 GGGCC
SfcI 195 CTACAG C^T[GA][CT]AG
360 CTACAG
XhoII 883 AGATCC [GA]^GATC[CT]
1147 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI ApoI Asp700I BbsI BpmI
BpuAI BsaJI BsrI BssKI BstNI
DdeI DpnII EcoRII HaeIII HinfI
HphI MboI MboII MnlI MseI
MvaI NdeII NlaIII Sau3AI ScrFI
SfaNI TfiI Tru9I Tsp509I TspRI
XmnI