Restriction map for
pF1KB9290
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BpuAI BsaBI BsaHI BsaI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CfoI
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI Eco47III EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
Ksp632I KspI MamI MboII MluI
MluNI MroI MscI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AclI 478 AACGTT AA^CGTT
Alw44I 113 GTGCAC G^TGCAC
AlwI 90 GGATC GGATC(4/5)
AocI 857 CCTCAGG CC^T[ACGT]AGG
ApaLI 113 GTGCAC G^TGCAC
AvaI 440 CCCGGG C^[CT]CG[GA]G
BanI 790 GGCACC G^G[CT][GA]CC
BanII 765 GGGCTC G[GA]GC[CT]^C
BbrPI 560 CACGTG CAC^GTG
BfaI 508 CTAG C^TAG
BglII 167 AGATCT A^GATCT
BlpI 385 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 385 GCTCAGC GC^T[ACGT]AGC
BsaAI 560 CACGTG [CT]AC^GT[GA]
BsaWI 411 ACCGGA [AT]^CCGG[AT]
BseRI 133 CTCCTC GAGGAG(10/8)
BsgI 142 GTGCAG GTGCAG(16/14)
BsiEI 726 CGGCCG CG[GA][CT]^CG
BsmAI 547 GAGAC GTCTC(1/5)
BsoBI 440 CCCGGG C^[CT]CG[GA]G
BspMI 605 GCAGGT ACCTGC(4/8)
BsrBI 729 CCGCTC CCGCTC(-3/-3)
BstXI 836 CCAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 167 AGATCT [GA]^GATC[CT]
Bsu36I 857 CCTCAGG CC^T[ACGT]AGG
CelII 385 GCTCAGC GC^T[ACGT]AGC
DsaI 704 CCATGG C^C[GA][CT]GG
EaeI 726 CGGCCG [CT]^GGCC[GA]
EagI 726 CGGCCG C^GGCCG
EclXI 726 CGGCCG C^GGCCG
Eco52I 726 CGGCCG C^GGCCG
Eco81I 857 CCTCAGG CC^T[ACGT]AGG
HincII 284 GTCAAC GT[CT]^[GA]AC
HindII 284 GTCAAC GT[CT]^[GA]AC
MaeI 508 CTAG C^TAG
MfeI 501 CAATTG C^AATTG
MflI 167 AGATCT [GA]^GATC[CT]
MunI 501 CAATTG C^AATTG
NcoI 704 CCATGG C^CATGG
PflMI 591 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 715 GAGTC GAGTC(4/5)
PmaCI 560 CACGTG CAC^GTG
PmlI 560 CACGTG CAC^GTG
Psp1406I 478 AACGTT AA^CGTT
SexAI 491 ACCTGGT A^CC[AT]GGT
SmaI 440 CCCGGG CCC^GGG
TaqI 453 TCGA T^CGA
Tsp509I 502 AATT ^AATT
TthHB8I 453 TCGA T^CGA
Van91I 591 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 62 CCATGAAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 167 AGATCT [GA]^GATC[CT]
XmaCI 440 CCCGGG C^CCGGG
XmaI 440 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BsrFI 668 ACCGGC [GA]^CCGG[CT]
724 ACCGGC
BstF5I 317 CATCC GGATG(2/0)
414 GGATG
Cac8I 221 GCGAGC GC[ACGT]^[ACGT]GC
584 GCCAGC
Cfr10I 668 ACCGGC [GA]^CCGG[CT]
724 ACCGGC
DpnII 91 GATC ^GATC
168 GATC
FokI 317 CATCC GGATG(9/13)
414 GGATG
MaeIII 665 GTGAC ^GT[ACGT]AC
697 GTGAC
MboI 91 GATC ^GATC
168 GATC
MseI 632 TTAA T^TAA
872 TTAA
MslI 322 CATAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
429 CACGTTGATG
MspA1I 160 CAGCTG C[AC]G^C[GT]G
878 CAGCTG
NdeII 91 GATC ^GATC
168 GATC
PvuII 160 CAGCTG CAG^CTG
878 CAGCTG
Sau3AI 91 GATC ^GATC
168 GATC
TfiI 450 GAATC G^A[AT]TC
816 GAATC
Tru9I 632 TTAA T^TAA
872 TTAA
Tsp45I 665 GTGAC ^GT[GC]AC
697 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BbvI BcnI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstNI Cfr13I DdeI
DraII EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI MaeII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
PpuMI Sau96I ScrFI StyI TseI
TspRI