Restriction map for
pF1KB5352
##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstPI
BstXI Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI Hin1I HindIII
HpaII KasI KpnI KspI MluI
MroI MspA1I MspI MwoI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tsp45I Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AflIII 1061 ACATGT A^C[GA][CT]GT
Asp700I 960 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 126 GGACC G^G[AT]CC
BalI 1253 TGGCCA TGG^CCA
BanII 920 GGGCTC G[GA]GC[CT]^C
BbsI 559 GAAGAC GAAGAC(2/6)
BbvCI 933 GCTGAGG CCTCAGC(-5/-2)
BglII 825 AGATCT A^GATCT
BmyI 920 GGGCTC G[AGT]GC[ACT]^C
BpmI 1088 CTCCAG CTGGAG(16/14)
BpuAI 559 GAAGAC GAAGAC(2/6)
BsaBI 43 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiYI 1245 CCAGATGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1245 CCAGATGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 1009 CGTCTC CGTCTC(1/5)
Bsp1286I 920 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 1061 ACATGT A^CATGT
Bst1107I 169 GTATAC GTA^TAC
BstBI 800 TTCGAA TT^CGAA
BstZ17I 169 GTATAC GTA^TAC
Cac8I 516 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 31 GCGC GCG^C
Cfr13I 126 GGACC G^G[ACGT]CC
DsaI 554 CCATGG C^C[GA][CT]GG
EaeI 1253 TGGCCA [CT]^GGCC[GA]
EcoT14I 554 CCATGG C^C[AT][AT]GG
HhaI 31 GCGC GCG^C
HinP1I 31 GCGC G^CGC
HpaI 901 GTTAAC GTT^AAC
MaeII 807 ACGT A^CGT
MaeIII 1181 GTAAC ^GT[ACGT]AC
MamI 43 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 269 CAATTG C^AATTG
MluNI 1253 TGGCCA TGG^CCA
MscI 1253 TGGCCA TGG^CCA
MslI 1056 CATTAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 269 CAATTG C^AATTG
NcoI 554 CCATGG C^CATGG
NruI 684 TCGCGA TCG^CGA
NspI 1061 ACATGT [GA]CATG^[CT]
NspV 800 TTCGAA TT^CGAA
PflMI 1245 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I 126 GGACC G^G[ACGT]CC
SfuI 800 TTCGAA TT^CGAA
StyI 554 CCATGG C^C[AT][AT]GG
TaqI 801 TCGA T^CGA
TthHB8I 801 TCGA T^CGA
Van91I 1245 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 411 CCAGTACGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 960 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 151 GTAGAC GT^[AC][GT]AC
169 GTATAC
AccII 30 CGCG CG^CG
685 CGCG
AfaI 9 GTAC GT^AC
414 GTAC
AhdI 741 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1026 GACTTTCTGTC
AlwI 289 GATCC GGATC(4/5)
42 GGATC
AspEI 741 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1026 GACTTTCTGTC
BbvI 439 GCAGC GCAGC(8/12)
550 GCAGC
BfaI 52 CTAG C^TAG
829 CTAG
BsaI 580 GAGACC GGTCTC(1/5)
650 GGTCTC
BsaJI 320 CCAGGG C^C[ACGT][ACGT]GG
554 CCATGG
BspMI 813 GCAGGT ACCTGC(4/8)
1289 GCAGGT
BssKI 320 CCAGG ^CC[ACGT]GG
348 CCTGG
BstNI 320 CCAGG CC^[AT]GG
348 CCTGG
BstUI 30 CGCG CG^CG
685 CGCG
BstYI 288 AGATCC [GA]^GATC[CT]
825 AGATCT
Eam1105I 741 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1026 GACTTTCTGTC
EcoRII 320 CCAGG ^CC[AT]GG
348 CCTGG
Fnu4HI 439 GCAGC GC^[ACGT]GC
550 GCAGC
HaeIII 519 GGCC GG^CC
1254 GGCC
HincII 315 GTCAAC GT[CT]^[GA]AC
901 GTTAAC
HindII 315 GTCAAC GT[CT]^[GA]AC
901 GTTAAC
HphI 672 TCACC GGTGA(8/7)
1195 TCACC
ItaI 439 GCAGC GC^[ACGT]GC
550 GCAGC
MaeI 52 CTAG C^TAG
829 CTAG
MflI 288 AGATCC [GA]^GATC[CT]
825 AGATCT
MvaI 320 CCAGG CC^[AT]GG
348 CCTGG
MvnI 30 CGCG CG^CG
685 CGCG
NlaIV 471 GGTTCC GG[ACGT]^[ACGT]CC
1299 GGCTCC
PleI 1086 GACTC GAGTC(4/5)
850 GAGTC
RsaI 9 GTAC GT^AC
414 GTAC
ScrFI 320 CCAGG CC^[ACGT]GG
348 CCTGG
SfcI 393 CTACAG C^T[GA][CT]AG
773 CTACAG
SmlI 985 CTTGAG C^T[CT][GA]AG
1316 CTCAAG
TseI 439 GCAGC G^C[AT]GC
550 GCAGC
XhoII 288 AGATCC [GA]^GATC[CT]
825 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AcsI AluI ApoI BsmAI
BsrI BstF5I DdeI DpnII EarI
FokI HinfI Ksp632I MboI MboII
MnlI MseI NdeII NlaIII Sau3AI
SfaNI SspI TfiI Tru9I Tsp509I
TspRI