Restriction map for
pF1KE1001
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI Ksp632I
KspI MaeI MaeII MaeIII MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tsp509I TspRI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 32 GGTACC G^GTACC
AfaI 33 GTAC GT^AC
AflIII 188 ACATGT A^C[GA][CT]GT
AlwI 147 GGATC GGATC(4/5)
AocI 129 CCTGAGG CC^T[ACGT]AGG
ApaI 134 GGGCCC GGGCC^C
Asp718I 32 GGTACC G^GTACC
AspHI 52 GAGCTC G[AT]GC[AT]^C
AvaI 10 CCCGAG C^[CT]CG[GA]G
AvaII 93 GGACC G^G[AT]CC
BanI 32 GGTACC G^G[CT][GA]CC
BbvI 27 GCTGC GCAGC(8/12)
BsaBI 238 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 324 ACCGGA [AT]^CCGG[AT]
BsiHKAI 52 GAGCTC G[AT]GC[AT]^C
BsmAI 214 GAGAC GTCTC(1/5)
BsoBI 10 CCCGAG C^[CT]CG[GA]G
BspLU11I 188 ACATGT A^CATGT
BstF5I 363 GGATG GGATG(2/0)
BstYI 147 GGATCT [GA]^GATC[CT]
Bsu36I 129 CCTGAGG CC^T[ACGT]AGG
Eco81I 129 CCTGAGG CC^T[ACGT]AGG
EcoT14I 315 CCAAGG C^C[AT][AT]GG
FokI 363 GGATG GGATG(9/13)
HapII 325 CCGG C^CGG
HincII 295 GTCAAC GT[CT]^[GA]AC
HindII 295 GTCAAC GT[CT]^[GA]AC
HpaII 325 CCGG C^CGG
KpnI 32 GGTACC GGTAC^C
MamI 238 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 163 GAAGA GAAGA(8/7)
MflI 147 GGATCT [GA]^GATC[CT]
MspI 325 CCGG C^CGG
MwoI 21 GCGAGTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 189 CATG CATG^
NspI 188 ACATGT [GA]CATG^[CT]
PstI 139 CTGCAG CTGCA^G
RsaI 33 GTAC GT^AC
SacI 52 GAGCTC GAGCT^C
SfaNI 201 GATGC GCATC(5/9)
SfcI 139 CTGCAG C^T[GA][CT]AG
SmlI 379 CTCAAG C^T[CT][GA]AG
StyI 315 CCAAGG C^C[AT][AT]GG
TaqI 120 TCGA T^CGA
TseI 27 GCTGC G^C[AT]GC
TthHB8I 120 TCGA T^CGA
XhoII 147 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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BanII 52 GAGCTC G[GA]GC[CT]^C
134 GGGCCC
BmyI 52 GAGCTC G[AGT]GC[ACT]^C
134 GGGCCC
BseRI 49 GAGGAG GAGGAG(10/8)
61 GAGGAG
Bsp1286I 52 GAGCTC G[AGT]GC[ACT]^C
134 GGGCCC
BssKI 96 CCTGG ^CC[ACGT]GG
360 CCAGG
BstNI 96 CCTGG CC^[AT]GG
360 CCAGG
Cac8I 54 GCTCGC GC[ACGT]^[ACGT]GC
69 GCTGGC
EcoRII 96 CCTGG ^CC[AT]GG
360 CCAGG
Fnu4HI 27 GCTGC GC^[ACGT]GC
81 GCGGC
HinfI 111 GAATC G^A[ACGT]TC
117 GAATC
HphI 7 TCACC GGTGA(8/7)
207 TCACC
ItaI 27 GCTGC GC^[ACGT]GC
81 GCGGC
MvaI 96 CCTGG CC^[AT]GG
360 CCAGG
NlaIV 32 GGTACC GG[ACGT]^[ACGT]CC
134 GGGCCC
ScrFI 96 CCTGG CC^[ACGT]GG
360 CCAGG
TfiI 111 GAATC G^A[AT]TC
117 GAATC
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI AluI BsaJI BsiYI BslI
Cfr13I DdeI DpnII DraII EcoNI
EcoO109I HaeIII MboI MnlI MseI
NdeII Sau3AI Sau96I Tru9I