Restriction map for
pF1KE1006
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BanI
BbeI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI HinP1I HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MboII MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 442 AAATTC [GA]^AATT[CT]
AfaI 540 GTAC GT^AC
AflIII 499 ACGTGT A^C[GA][CT]GT
ApoI 442 AAATTC [GA]^AATT[CT]
AspHI 475 GAGCTC G[AT]GC[AT]^C
AvaI 324 CTCGGG C^[CT]CG[GA]G
BamHI 231 GGATCC G^GATCC
BbrPI 498 CACGTG CAC^GTG
BpmI 425 CTGGAG CTGGAG(16/14)
BsaAI 498 CACGTG [CT]AC^GT[GA]
BsaI 428 GAGACC GGTCTC(1/5)
BsiHKAI 475 GAGCTC G[AT]GC[AT]^C
BsmAI 428 GAGAC GTCTC(1/5)
BsoBI 324 CTCGGG C^[CT]CG[GA]G
BsrDI 281 GCAATG GCAATG(2/0)
BsrFI 3 GCCGGT [GA]^CCGG[CT]
BstAPI 66 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 231 GGATCC [GA]^GATC[CT]
Cfr10I 3 GCCGGT [GA]^CCGG[CT]
DsaI 435 CCGTGG C^C[GA][CT]GG
FauI 69 CCCGC CCCGC(4/6)
HincII 268 GTCAAC GT[CT]^[GA]AC
HindII 268 GTCAAC GT[CT]^[GA]AC
HinfI 175 GATTC G^A[ACGT]TC
HphI 80 TCACC GGTGA(8/7)
MaeII 499 ACGT A^CGT
MflI 231 GGATCC [GA]^GATC[CT]
MseI 137 TTAA T^TAA
MslI 352 CACCGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 75 GCATGT [GA]CATG^[CT]
PmaCI 498 CACGTG CAC^GTG
PmlI 498 CACGTG CAC^GTG
PvuII 547 CAGCTG CAG^CTG
RsaI 540 GTAC GT^AC
SacI 475 GAGCTC GAGCT^C
SmlI 33 CTTGAG C^T[CT][GA]AG
StuI 345 AGGCCT AGG^CCT
TfiI 175 GATTC G^A[AT]TC
Tru9I 137 TTAA T^TAA
Tsp509I 443 AATT ^AATT
TspRI 294 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 231 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 123 GACGCC G[GA]^CG[CT]C
453 GGCGTC
AlwI 232 GATCC GGATC(4/5)
231 GGATC
ApaI 28 GGGCCC GGGCC^C
482 GGGCCC
BbvCI 12 CCTCAGC CCTCAGC(-5/-2)
411 CCTCAGC
BglI 88 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
484 GCCCCGGTGGC
BsaHI 123 GACGCC G[GA]^CG[CT]C
453 GGCGTC
Cac8I 38 GCCTGC GC[ACGT]^[ACGT]GC
493 GCTGGC
DpnII 232 GATC ^GATC
422 GATC
EcoT14I 194 CCTTGG C^C[AT][AT]GG
335 CCAAGG
HgaI 123 GACGC GACGC(5/10)
454 GCGTC
Hin1I 123 GACGCC G[GA]^CG[CT]C
453 GGCGTC
MboI 232 GATC ^GATC
422 GATC
MspA1I 70 CCGCTG C[AC]G^C[GT]G
547 CAGCTG
NdeII 232 GATC ^GATC
422 GATC
NlaIII 76 CATG CATG^
273 CATG
Sau3AI 232 GATC ^GATC
422 GATC
SfaNI 214 GATGC GCATC(5/9)
252 GCATC
StyI 194 CCTTGG C^C[AT][AT]GG
335 CCAAGG
Tsp45I 79 GTCAC ^GT[GC]AC
398 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cfr13I
DdeI DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HpaII ItaI
MaeIII MnlI MspI MvaI MwoI
NciI NlaIV Sau96I ScrFI TseI