Restriction map for
pF1KB5413
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
DraI DrdI DsaI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FseI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI SacII SalI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tsp509I Tth111I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccIII 515 TCCGGA T^CCGGA
AfaI 258 GTAC GT^AC
AluI 446 AGCT AG^CT
AlwI 395 GGATC GGATC(4/5)
AlwNI 309 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 445 GAGCTC G[AT]GC[AT]^C
AvaI 408 CTCGAG C^[CT]CG[GA]G
BalI 305 TGGCCA TGG^CCA
BanII 445 GAGCTC G[GA]GC[CT]^C
BbsI 58 GTCTTC GAAGAC(2/6)
BclI 581 TGATCA T^GATCA
BlpI 123 GCTCAGC GC^T[ACGT]AGC
BpmI 263 CTGGAG CTGGAG(16/14)
Bpu1102I 123 GCTCAGC GC^T[ACGT]AGC
BpuAI 58 GTCTTC GAAGAC(2/6)
BseAI 515 TCCGGA T^CCGGA
BsgI 319 CTGCAC GTGCAG(16/14)
BsiEI 587 CGGCCG CG[GA][CT]^CG
BsiHKAI 445 GAGCTC G[AT]GC[AT]^C
BsoBI 408 CTCGAG C^[CT]CG[GA]G
BspEI 515 TCCGGA T^CCGGA
BspMI 119 ACCTGC ACCTGC(4/8)
BstYI 395 GGATCT [GA]^GATC[CT]
CelII 123 GCTCAGC GC^T[ACGT]AGC
CpoI 212 CGGACCG CG^G[AT]CCG
DraII 486 GGGACCT [GA]G^G[ACGT]CC[CT]
EagI 587 CGGCCG C^GGCCG
EarI 448 CTCTTC CTCTTC(1/4)
EclXI 587 CGGCCG C^GGCCG
Eco52I 587 CGGCCG C^GGCCG
EcoO109I 486 GGGACCT [GA]G^G[ACGT]CC[CT]
FauI 593 CCCGC CCCGC(4/6)
FbaI 581 TGATCA T^GATCA
HincII 241 GTCAAC GT[CT]^[GA]AC
HindII 241 GTCAAC GT[CT]^[GA]AC
HphI 425 TCACC GGTGA(8/7)
Ksp632I 448 CTCTTC CTCTTC(1/4)
MflI 395 GGATCT [GA]^GATC[CT]
MluNI 305 TGGCCA TGG^CCA
MroI 515 TCCGGA T^CCGGA
MscI 305 TGGCCA TGG^CCA
MseI 170 TTAA T^TAA
NspI 203 ACATGC [GA]CATG^[CT]
PaeR7I 408 CTCGAG C^TCGAG
PflMI 308 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 354 GAGTC GAGTC(4/5)
PpuMI 486 GGGACCT [GA]G^G[AT]CC[CT]
RsaI 258 GTAC GT^AC
RsrII 212 CGGACCG CG^G[AT]CCG
SacI 445 GAGCTC GAGCT^C
SapI 447 GCTCTTC GCTCTTC(1/4)
SmlI 408 CTCGAG C^T[CT][GA]AG
StuI 412 AGGCCT AGG^CCT
TfiI 381 GAATC G^A[AT]TC
Tru9I 170 TTAA T^TAA
Van91I 308 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 227 CCATGTGCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 408 CTCGAG C^TCGAG
XhoII 395 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 163 CGCG CG^CG
625 CGCG
BmyI 28 GTGCCC G[AGT]GC[ACT]^C
445 GAGCTC
BsaWI 384 TCCGGT [AT]^CCGG[AT]
515 TCCGGA
BseRI 108 CTCCTC GAGGAG(10/8)
460 CTCCTC
BsmFI 486 GGGAC GGGAC(10/14)
507 GGGAC
Bsp1286I 28 GTGCCC G[AGT]GC[ACT]^C
445 GAGCTC
BsrI 314 ACTGG ACTGG(1/-1)
308 CCAGT
BstUI 163 CGCG CG^CG
625 CGCG
CfoI 164 GCGC GCG^C
626 GCGC
DdeI 124 CTCAG C^T[ACGT]AG
482 CTGAG
DraIII 225 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
426 CACCTTGTG
EaeI 305 TGGCCA [CT]^GGCC[GA]
587 CGGCCG
HhaI 164 GCGC GCG^C
626 GCGC
HinP1I 164 GCGC G^CGC
626 GCGC
HinfI 354 GAGTC G^A[ACGT]TC
381 GAATC
MspA1I 126 CAGCGG C[AC]G^C[GT]G
216 CCGCTG
MvnI 163 CGCG CG^CG
625 CGCG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AvaII BbvI BcnI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cac8I Cfr13I DpnII EcoRII Fnu4HI
FokI HaeIII HapII HgaI HpaII
ItaI MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TaqI TseI Tsp45I TspRI TthHB8I