Restriction map for
pF1KB5412
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BfrI
BglI BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CfoI ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MunI NaeI
NarI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 613 CGCG CG^CG
Asp700I 400 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 145 GAGCTC G[AT]GC[AT]^C
AvaI 79 CCCGAG C^[CT]CG[GA]G
BanII 145 GAGCTC G[GA]GC[CT]^C
BclI 492 TGATCA T^GATCA
BcnI 6 CCGGG CC^[GC]GG
BglII 864 AGATCT A^GATCT
BlpI 540 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 540 GCTAAGC GC^T[ACGT]AGC
BsaI 137 GAGACC GGTCTC(1/5)
BsiHKAI 145 GAGCTC G[AT]GC[AT]^C
BsoBI 79 CCCGAG C^[CT]CG[GA]G
BsrDI 692 GCAATG GCAATG(2/0)
BsrFI 39 GCCGGT [GA]^CCGG[CT]
BstUI 613 CGCG CG^CG
BstYI 864 AGATCT [GA]^GATC[CT]
CelII 540 GCTAAGC GC^T[ACGT]AGC
Cfr10I 39 GCCGGT [GA]^CCGG[CT]
EcoT14I 376 CCATGG C^C[AT][AT]GG
FauI 133 GCGGG CCCGC(4/6)
FbaI 492 TGATCA T^GATCA
HgaI 614 GCGTC GACGC(5/10)
HincII 382 GTTGAC GT[CT]^[GA]AC
HindII 382 GTTGAC GT[CT]^[GA]AC
HphI 987 GGTGA GGTGA(8/7)
MflI 864 AGATCT [GA]^GATC[CT]
MvnI 613 CGCG CG^CG
NciI 6 CCGGG CC^[GC]GG
NcoI 376 CCATGG C^CATGG
PleI 106 GAGTC GAGTC(4/5)
SacI 145 GAGCTC GAGCT^C
SpeI 888 ACTAGT A^CTAGT
StyI 376 CCATGG C^C[AT][AT]GG
TspRI 828 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 864 AGATCT [GA]^GATC[CT]
XmnI 400 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 224 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
992 CAGAATCTG
BfaI 803 CTAG C^TAG
889 CTAG
BmyI 145 GAGCTC G[AGT]GC[ACT]^C
370 GTGCCC
BpmI 523 CTCCAG CTGGAG(16/14)
594 CTGGAG
BsmBI 615 CGTCTC CGTCTC(1/5)
94 GAGACG
Bsp1286I 145 GAGCTC G[AGT]GC[ACT]^C
370 GTGCCC
BsrI 924 ACTGG ACTGG(1/-1)
1016 ACTGG
BstF5I 531 GGATG GGATG(2/0)
783 GGATG
DpnII 493 GATC ^GATC
865 GATC
DsaI 376 CCATGG C^C[GA][CT]GG
506 CCGTGG
EcoNI 150 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
852 CCTAAATCAGG
FokI 531 GGATG GGATG(9/13)
783 GGATG
MaeI 803 CTAG C^TAG
889 CTAG
MboI 493 GATC ^GATC
865 GATC
NdeII 493 GATC ^GATC
865 GATC
NlaIII 377 CATG CATG^
519 CATG
PvuII 365 CAGCTG CAG^CTG
431 CAGCTG
SapI 337 GAAGAGC GCTCTTC(1/4)
687 GAAGAGC
Sau3AI 493 GATC ^GATC
865 GATC
SmlI 12 CTTGAG C^T[CT][GA]AG
776 CTCAAG
TaqI 485 TCGA T^CGA
839 TCGA
Tsp45I 455 GTGAC ^GT[GC]AC
988 GTGAC
TthHB8I 485 TCGA T^CGA
839 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BsaJI BseRI BsiYI BslI
BsmAI BssKI BstAPI BstNI Cac8I
Cfr13I DdeI EarI EcoRII Fnu4HI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeIII MboII MnlI MseI
MspA1I MspI MvaI MwoI PstI
RsaI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp509I