Restriction map for
pF1KB8735
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
AlwI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI BsrI
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI StuI StyI
SwaI Tru9I Tsp509I TspRI Tth111I
Van91I XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AlwNI 145 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 313 GGGCCC GGGCC^C
BbsI 715 GTCTTC GAAGAC(2/6)
BfaI 160 CTAG C^TAG
BlpI 741 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 741 GCTCAGC GC^T[ACGT]AGC
BpuAI 715 GTCTTC GAAGAC(2/6)
BseRI 127 GAGGAG GAGGAG(10/8)
BsiEI 699 CGACCG CG[GA][CT]^CG
BsmFI 9 GTCCC GGGAC(10/14)
BspLU11I 437 ACATGT A^CATGT
BsrBI 607 CCGCTC CCGCTC(-3/-3)
BssHII 153 GCGCGC G^CGCGC
BstXI 400 CCACGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 741 GCTCAGC GC^T[ACGT]AGC
DpnII 537 GATC ^GATC
HgaI 777 GCGTC GACGC(5/10)
HphI 3 GGTGA GGTGA(8/7)
MaeI 160 CTAG C^TAG
MboI 537 GATC ^GATC
NdeII 537 GATC ^GATC
NlaIII 438 CATG CATG^
NspI 437 ACATGT [GA]CATG^[CT]
PaeR7I 181 CTCGAG C^TCGAG
PvuII 723 CAGCTG CAG^CTG
SacI 739 GAGCTC GAGCT^C
SapI 609 GCTCTTC GCTCTTC(1/4)
Sau3AI 537 GATC ^GATC
SbfI 690 CCTGCAGG CCTGCA^GG
SfaNI 276 GCATC GCATC(5/9)
SmaI 207 CCCGGG CCC^GGG
SmlI 181 CTCGAG C^T[CT][GA]AG
Sse8387I 690 CCTGCAGG CCTGCA^GG
TfiI 430 GAATC G^A[AT]TC
Tsp45I 541 GTCAC ^GT[GC]AC
XhoI 181 CTCGAG C^TCGAG
XmaCI 207 CCCGGG C^CCGGG
XmaI 207 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 23 ACACGT A^C[GA][CT]GT
437 ACATGT
Alw44I 286 GTGCAC G^TGCAC
292 GTGCAC
ApaLI 286 GTGCAC G^TGCAC
292 GTGCAC
AvaI 181 CTCGAG C^[CT]CG[GA]G
207 CCCGGG
AvaII 618 GGACC G^G[AT]CC
728 GGACC
BglI 203 GCCCCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
396 GCCGCCACGGC
BsaI 64 GGTCTC GGTCTC(1/5)
786 GGTCTC
BsgI 304 CTGCAC GTGCAG(16/14)
640 CTGCAC
BsmAI 65 GTCTC GTCTC(1/5)
787 GTCTC
BsoBI 181 CTCGAG C^[CT]CG[GA]G
207 CCCGGG
BspMI 149 ACCTGC ACCTGC(4/8)
644 ACCTGC
BstAPI 144 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
294 GCACCCGCTGC
BstF5I 277 CATCC GGATG(2/0)
792 CATCC
EaeI 194 TGGCCG [CT]^GGCC[GA]
705 TGGCCG
EarI 610 CTCTTC CTCTTC(1/4)
460 GAAGAG
FokI 277 CATCC GGATG(9/13)
792 CATCC
Ksp632I 610 CTCTTC CTCTTC(1/4)
460 GAAGAG
MaeIII 455 GTTAC ^GT[ACGT]AC
541 GTCAC
MspA1I 298 CCGCTG C[AC]G^C[GT]G
723 CAGCTG
NaeI 43 GCCGGC GCC^GGC
258 GCCGGC
NgoMIV 43 GCCGGC G^CCGGC
258 GCCGGC
PleI 733 GACTC GAGTC(4/5)
555 GAGTC
PstI 90 CTGCAG CTGCA^G
691 CTGCAG
TaqI 176 TCGA T^CGA
182 TCGA
TthHB8I 176 TCGA T^CGA
182 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
BanI BanII BbeI BbrPI BbvI
BcnI BmyI BpmI BsaAI BsaHI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrFI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DsaI
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI MaeII MboII
MnlI MspI MvaI MvnI MwoI
NarI NciI NlaIV PmaCI PmlI
Sau96I ScrFI SfcI SfoI TseI