Restriction map for
pF1KB5797
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccIII AflII AhdI AocI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BbeI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BsrBI
BsrDI BsrGI BssHII BstBI Bsu36I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoRI
EcoT22I FbaI FseI FspI HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshBI PvuI RsrII SacI
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI SspBI SspI SwaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 523 GACGTC GACGT^C
AccII 2216 CGCG CG^CG
AclI 2496 AACGTT AA^CGTT
AcsI 347 GAATTT [GA]^AATT[CT]
AflIII 1136 ACATGT A^C[GA][CT]GT
Aor51HI 2474 AGCGCT AGC^GCT
ApaI 439 GGGCCC GGGCC^C
ApoI 347 GAATTT [GA]^AATT[CT]
AspI 37 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1212 GGATCC G^GATCC
BbrPI 445 CACGTG CAC^GTG
BcgI 222 CGAGCAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 2453 CTAG C^TAG
BsaI 2146 GAGACC GGTCTC(1/5)
BsiEI 147 CGGTCG CG[GA][CT]^CG
BspLU11I 1136 ACATGT A^CATGT
BssSI 220 CACGAG CACGAG(-5/-1)
BstAPI 1411 GCAAATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1774 GGTGACC G^GT[ACGT]ACC
BstPI 1774 GGTGACC G^GT[ACGT]ACC
BstUI 2216 CGCG CG^CG
CfoI 2475 GCGC GCG^C
Eco47III 2474 AGCGCT AGC^GCT
EcoO65I 1774 GGTGACC G^GT[ACGT]ACC
EcoRV 532 GATATC GAT^ATC
HaeII 2474 AGCGCT [GA]GCGC^[CT]
HhaI 2475 GCGC GCG^C
HinP1I 2475 GCGC G^CGC
HindIII 181 AAGCTT A^AGCTT
MaeI 2453 CTAG C^TAG
MseI 1516 TTAA T^TAA
MvnI 2216 CGCG CG^CG
NcoI 483 CCATGG C^CATGG
NdeI 947 CATATG CA^TATG
NspI 1136 ACATGT [GA]CATG^[CT]
PflFI 37 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 445 CACGTG CAC^GTG
PmlI 445 CACGTG CAC^GTG
PpuMI 818 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2364 GACCATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2496 AACGTT AA^CGTT
SapI 1203 GCTCTTC GCTCTTC(1/4)
SbfI 2418 CCTGCAGG CCTGCA^GG
SnaBI 490 TACGTA TAC^GTA
Sse8387I 2418 CCTGCAGG CCTGCA^GG
StuI 1815 AGGCCT AGG^CCT
Tru9I 1516 TTAA T^TAA
Tsp509I 348 AATT ^AATT
Tth111I 37 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2452 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 493 GTATAC GT^[AC][GT]AC
562 GTATAC
AcyI 523 GACGTC G[GA]^CG[CT]C
1621 GACGCC
AgeI 864 ACCGGT A^CCGGT
1586 ACCGGT
Alw44I 2255 GTGCAC G^TGCAC
2431 GTGCAC
ApaLI 2255 GTGCAC G^TGCAC
2431 GTGCAC
BanII 439 GGGCCC G[GA]GC[CT]^C
930 GGGCTC
BbsI 1477 GAAGAC GAAGAC(2/6)
1146 GTCTTC
BlpI 1995 GCTGAGC GC^T[ACGT]AGC
2201 GCTCAGC
Bpu1102I 1995 GCTGAGC GC^T[ACGT]AGC
2201 GCTCAGC
BpuAI 1477 GAAGAC GAAGAC(2/6)
1146 GTCTTC
BsaAI 445 CACGTG [CT]AC^GT[GA]
490 TACGTA
BsaHI 523 GACGTC G[GA]^CG[CT]C
1621 GACGCC
BspMI 796 ACCTGC ACCTGC(4/8)
2295 GCAGGT
BsrFI 864 ACCGGT [GA]^CCGG[CT]
1586 ACCGGT
Bst1107I 493 GTATAC GTA^TAC
562 GTATAC
BstXI 1274 CCAGCCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2366 CCATAGTCATGG
BstYI 1212 GGATCC [GA]^GATC[CT]
1490 AGATCC
BstZ17I 493 GTATAC GTA^TAC
562 GTATAC
CelII 1995 GCTGAGC GC^T[ACGT]AGC
2201 GCTCAGC
Cfr10I 864 ACCGGT [GA]^CCGG[CT]
1586 ACCGGT
DraIII 1739 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
2434 CACCAAGTG
EcoNI 652 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1818 CCTGAGCAAGG
EcoT14I 483 CCATGG C^C[AT][AT]GG
1160 CCAAGG
Hin1I 523 GACGTC G[GA]^CG[CT]C
1621 GACGCC
MflI 1212 GGATCC [GA]^GATC[CT]
1490 AGATCC
PflMI 1738 CCACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2007 CCAGCTGTTGG
PinAI 864 ACCGGT A^CCGGT
1586 ACCGGT
PstI 857 CTGCAG CTGCA^G
2419 CTGCAG
StyI 483 CCATGG C^C[AT][AT]GG
1160 CCAAGG
TaqI 873 TCGA T^CGA
2053 TCGA
TthHB8I 873 TCGA T^CGA
2053 TCGA
Van91I 1738 CCACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2007 CCAGCTGTTGG
XcmI 354 CCAAGTCCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1014 CCAGGAAAGGGATGG
XhoII 1212 GGATCC [GA]^GATC[CT]
1490 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanI
BbvI BcnI BmyI BpmI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TfiI TseI Tsp45I
TspRI