Restriction map for
pF1KB9657
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI HphI
KasI KpnI MaeI MaeIII MamI
MfeI MflI MluI MroI MseI
MslI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tsp45I Tsp509I TspRI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcyI 175 GGCGTC G[GA]^CG[CT]C
AfaI 462 GTAC GT^AC
AflIII 11 ACGTGT A^C[GA][CT]GT
AlwNI 364 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 151 GGGCCC GGGCC^C
BalI 335 TGGCCA TGG^CCA
BanII 151 GGGCCC G[GA]GC[CT]^C
BglI 69 GCCAGACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 151 GGGCCC G[AGT]GC[ACT]^C
BsaHI 175 GGCGTC G[GA]^CG[CT]C
BseRI 310 GAGGAG GAGGAG(10/8)
BsiEI 35 CGGCCG CG[GA][CT]^CG
BsmAI 28 GTCTC GTCTC(1/5)
Bsp1286I 151 GGGCCC G[AGT]GC[ACT]^C
BssSI 538 CACGAG CACGAG(-5/-1)
DpnII 399 GATC ^GATC
DsaI 164 CCGCGG C^C[GA][CT]GG
EagI 35 CGGCCG C^GGCCG
EarI 64 GAAGAG CTCTTC(1/4)
EclXI 35 CGGCCG C^GGCCG
Eco52I 35 CGGCCG C^GGCCG
EcoNI 474 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 350 AGCGCC [GA]GCGC^[CT]
Hin1I 175 GGCGTC G[GA]^CG[CT]C
Ksp632I 64 GAAGAG CTCTTC(1/4)
KspI 164 CCGCGG CCGC^GG
MboI 399 GATC ^GATC
MluNI 335 TGGCCA TGG^CCA
MscI 335 TGGCCA TGG^CCA
NaeI 33 GCCGGC GCC^GGC
NdeII 399 GATC ^GATC
NgoMIV 33 GCCGGC G^CCGGC
PpuMI 482 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 462 GTAC GT^AC
SacII 164 CCGCGG CCGC^GG
SapI 64 GAAGAGC GCTCTTC(1/4)
Sau3AI 399 GATC ^GATC
SexAI 479 ACCAGGT A^CC[AT]GGT
SfaNI 3 GATGC GCATC(5/9)
SmlI 445 CTCAAG C^T[CT][GA]AG
TaqI 467 TCGA T^CGA
TfiI 435 GATTC G^A[AT]TC
TthHB8I 467 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 483 GGTCC G^G[AT]CC
587 GGTCC
BlpI 426 GCTGAGC GC^T[ACGT]AGC
546 GCTCAGC
BpmI 487 CTCCAG CTGGAG(16/14)
567 CTGGAG
Bpu1102I 426 GCTGAGC GC^T[ACGT]AGC
546 GCTCAGC
BsmFI 105 GGGAC GGGAC(10/14)
600 GTCCC
BsrBI 90 GAGCGG CCGCTC(-3/-3)
541 GAGCGG
BsrFI 33 GCCGGC [GA]^CCGG[CT]
74 ACCGGC
BsrI 25 CCAGT ACTGG(1/-1)
363 CCAGT
BstF5I 402 CATCC GGATG(2/0)
572 GGATG
CelII 426 GCTGAGC GC^T[ACGT]AGC
546 GCTCAGC
Cfr10I 33 GCCGGC [GA]^CCGG[CT]
74 ACCGGC
FokI 402 CATCC GGATG(9/13)
572 GGATG
MaeII 11 ACGT A^CGT
341 ACGT
MboII 64 GAAGA GAAGA(8/7)
396 GAAGA
NlaIII 333 CATG CATG^
591 CATG
PleI 505 GACTC GAGTC(4/5)
294 GAGTC
PstI 316 CTGCAG CTGCA^G
522 CTGCAG
SfcI 316 CTGCAG C^T[GA][CT]AG
522 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BsaJI BsiYI BslI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII ItaI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV Sau96I ScrFI TseI