Restriction map for
pF1KE4564
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BanI BbeI
BbrPI BbsI BcgI BclI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT14I FbaI FseI
FspI HaeII Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccI 418 GTATAC GT^[AC][GT]AC
AflIII 366 ACACGT A^C[GA][CT]GT
AocI 1573 CCTGAGG CC^T[ACGT]AGG
ApaI 274 GGGCCC GGGCC^C
BamHI 883 GGATCC G^GATCC
BbvCI 1296 CCTCAGC CCTCAGC(-5/-2)
BciVI 1892 GGATAC GTATCC(6/5)
BglII 381 AGATCT A^GATCT
BsaAI 891 TACGTG [CT]AC^GT[GA]
BsaI 2044 GGTCTC GGTCTC(1/5)
BspHI 2072 TCATGA T^CATGA
Bst1107I 418 GTATAC GTA^TAC
BstZ17I 418 GTATAC GTA^TAC
Bsu36I 1573 CCTGAGG CC^T[ACGT]AGG
CfoI 672 GCGC GCG^C
Eco81I 1573 CCTGAGG CC^T[ACGT]AGG
EcoNI 41 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 311 ATGCAT ATGCA^T
HhaI 672 GCGC GCG^C
HinP1I 672 GCGC G^CGC
HincII 1201 GTTGAC GT[CT]^[GA]AC
HindII 1201 GTTGAC GT[CT]^[GA]AC
MslI 864 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 311 ATGCAT ATGCA^T
RcaI 2072 TCATGA T^CATGA
ScaI 803 AGTACT AGT^ACT
TaqI 291 TCGA T^CGA
TthHB8I 291 TCGA T^CGA
XcmI 177 CCAGCATCTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 201 GTAC GT^AC
804 GTAC
AspHI 847 GAGCTC G[AT]GC[AT]^C
1381 GAGCTC
AspI 1213 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1747 GACCCAGTC
BalI 405 TGGCCA TGG^CCA
1452 TGGCCA
BpmI 447 CTCCAG CTGGAG(16/14)
1515 CTCCAG
BsgI 797 CTGCAC GTGCAG(16/14)
1331 CTGCAC
BsiHKAI 847 GAGCTC G[AT]GC[AT]^C
1381 GAGCTC
BstYI 381 AGATCT [GA]^GATC[CT]
883 GGATCC
Cac8I 276 GCCCGC GC[ACGT]^[ACGT]GC
631 GCAAGC
HgaI 517 GACGC GACGC(5/10)
1693 GACGC
MaeII 368 ACGT A^CGT
892 ACGT
MboII 394 GAAGA GAAGA(8/7)
1407 GAAGA
MflI 381 AGATCT [GA]^GATC[CT]
883 GGATCC
MluNI 405 TGGCCA TGG^CCA
1452 TGGCCA
MscI 405 TGGCCA TGG^CCA
1452 TGGCCA
MseI 1027 TTAA T^TAA
1195 TTAA
NlaIII 1312 CATG CATG^
2073 CATG
PflFI 1213 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1747 GACCCAGTC
PshAI 930 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1464 GACTACAGTC
RsaI 201 GTAC GT^AC
804 GTAC
SacI 847 GAGCTC GAGCT^C
1381 GAGCTC
Tru9I 1027 TTAA T^TAA
1195 TTAA
Tth111I 1213 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1747 GACCCAGTC
XhoII 381 AGATCT [GA]^GATC[CT]
883 GGATCC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIV PleI PpuMI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI TseI Tsp45I
Tsp509I TspRI