Restriction map for
pF1KB5863
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BbvCI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FspI HgaI Hin1I
HindIII HpaI KasI KpnI MamI
MluI MroI MseI MslI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsaI RsrII SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 259 GTCGAC GT^[AC][GT]AC
ApaI 1210 GGGCCC GGGCC^C
AvaI 1171 CTCGGG C^[CT]CG[GA]G
BalI 32 TGGCCA TGG^CCA
BbrPI 327 CACGTG CAC^GTG
BcgI 326 GCACGTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 960 CCCGG CC^[GC]GG
BfaI 454 CTAG C^TAG
BglII 185 AGATCT A^GATCT
BsaAI 327 CACGTG [CT]AC^GT[GA]
BsaI 1006 GAGACC GGTCTC(1/5)
BsoBI 1171 CTCGGG C^[CT]CG[GA]G
BspHI 1127 TCATGA T^CATGA
BsrBI 166 GAGCGG CCGCTC(-3/-3)
BssSI 1012 CTCGTG CACGAG(-5/-1)
BstEII 265 GGTGACC G^GT[ACGT]ACC
BstPI 265 GGTGACC G^GT[ACGT]ACC
DraIII 100 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1368 GAAGAG CTCTTC(1/4)
EcoNI 453 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 265 GGTGACC G^GT[ACGT]ACC
FseI 996 GGCCGGCC GGCCGG^CC
HincII 259 GTCGAC GT[CT]^[GA]AC
HindII 259 GTCGAC GT[CT]^[GA]AC
Ksp632I 1368 GAAGAG CTCTTC(1/4)
KspI 628 CCGCGG CCGC^GG
MaeI 454 CTAG C^TAG
MfeI 414 CAATTG C^AATTG
MluNI 32 TGGCCA TGG^CCA
MscI 32 TGGCCA TGG^CCA
MunI 414 CAATTG C^AATTG
NaeI 997 GCCGGC GCC^GGC
NciI 960 CCCGG CC^[GC]GG
NgoMIV 997 GCCGGC G^CCGGC
NspI 1151 ACATGC [GA]CATG^[CT]
PmaCI 327 CACGTG CAC^GTG
PmlI 327 CACGTG CAC^GTG
PstI 517 CTGCAG CTGCA^G
PvuII 354 CAGCTG CAG^CTG
RcaI 1127 TCATGA T^CATGA
SacII 628 CCGCGG CCGC^GG
SalI 259 GTCGAC G^TCGAC
SapI 1368 GAAGAGC GCTCTTC(1/4)
TfiI 1092 GATTC G^A[AT]TC
XcmI 128 CCAACTCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 44 GAATTT [GA]^AATT[CT]
212 AAATTT
ApoI 44 GAATTT [GA]^AATT[CT]
212 AAATTT
BamHI 113 GGATCC G^GATCC
1331 GGATCC
BanI 1234 GGCACC G^G[CT][GA]CC
1377 GGCACC
BglI 365 GCCGCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
709 GCCTGCGGGGC
BspMI 806 ACCTGC ACCTGC(4/8)
675 GCAGGT
BsrFI 997 GCCGGC [GA]^CCGG[CT]
1178 ACCGGC
Cfr10I 997 GCCGGC [GA]^CCGG[CT]
1178 ACCGGC
DdeI 72 CTCAG C^T[ACGT]AG
1145 CTGAG
DraII 553 AGGCCCT [GA]G^G[ACGT]CC[CT]
1209 GGGGCCC
EaeI 32 TGGCCA [CT]^GGCC[GA]
999 CGGCCA
EcoO109I 553 AGGCCCT [GA]G^G[ACGT]CC[CT]
1209 GGGGCCC
HaeII 473 GGCGCT [GA]GCGC^[CT]
812 GGCGCT
NcoI 1214 CCATGG C^CATGG
1335 CCATGG
SacI 145 GAGCTC GAGCT^C
985 GAGCTC
SexAI 101 ACCAGGT A^CC[AT]GGT
1237 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BanII BbsI BbvI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DpnII DsaI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PflMI PleI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII