Restriction map for
pF1KE6728
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BcnI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaI BsaJI BsaWI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I FauI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HindIII HpaII KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StyI SwaI Tth111I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AflII 1533 CTTAAG C^TTAAG
BbsI 1156 GAAGAC GAAGAC(2/6)
BbvCI 82 GCTGAGG CCTCAGC(-5/-2)
BclI 1410 TGATCA T^GATCA
BfrI 1533 CTTAAG C^TTAAG
BpmI 219 CTGGAG CTGGAG(16/14)
BpuAI 1156 GAAGAC GAAGAC(2/6)
BsaAI 2367 CACGTA [CT]AC^GT[GA]
BsgI 272 GTGCAG GTGCAG(16/14)
BsmBI 222 GAGACG CGTCTC(1/5)
BspHI 2642 TCATGA T^CATGA
BspLU11I 1977 ACATGT A^CATGT
EarI 2561 CTCTTC CTCTTC(1/4)
EcoNI 2656 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1732 ATGCAT ATGCA^T
FbaI 1410 TGATCA T^GATCA
HgaI 1478 GACGC GACGC(5/10)
Ksp632I 2561 CTCTTC CTCTTC(1/4)
MspA1I 80 CAGCTG C[AC]G^C[GT]G
NdeI 1790 CATATG CA^TATG
NsiI 1732 ATGCAT ATGCA^T
PvuII 80 CAGCTG CAG^CTG
RcaI 2642 TCATGA T^CATGA
SacI 1387 GAGCTC GAGCT^C
SpeI 2407 ACTAGT A^CTAGT
StuI 2628 AGGCCT AGG^CCT
XbaI 1284 TCTAGA T^CTAGA
XcmI 149 CCAACCACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AflIII 225 ACGTGT A^C[GA][CT]GT
1977 ACATGT
AlwNI 742 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1696 CAGTTACTG
BanII 277 GGGCTC G[GA]GC[CT]^C
1387 GAGCTC
BsaBI 844 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1405 GATTTTGATC
BsmFI 362 GTCCC GGGAC(10/14)
2862 GTCCC
BssKI 115 CCAGG ^CC[ACGT]GG
414 CCAGG
BstNI 115 CCAGG CC^[AT]GG
414 CCAGG
BstXI 464 CCAAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1313 CCACAAATGTGG
BstYI 3 GGATCT [GA]^GATC[CT]
2652 AGATCC
DraII 1308 AGGACCC [GA]G^G[ACGT]CC[CT]
2774 AGGACCC
EcoO109I 1308 AGGACCC [GA]G^G[ACGT]CC[CT]
2774 AGGACCC
EcoRII 115 CCAGG ^CC[AT]GG
414 CCAGG
MamI 844 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1405 GATTTTGATC
MflI 3 GGATCT [GA]^GATC[CT]
2652 AGATCC
MslI 1314 CACAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1725 CACAAAGATG
MvaI 115 CCAGG CC^[AT]GG
414 CCAGG
PpuMI 1308 AGGACCC [GA]G^G[AT]CC[CT]
2774 AGGACCC
PstI 1702 CTGCAG CTGCA^G
2797 CTGCAG
ScrFI 115 CCAGG CC^[ACGT]GG
414 CCAGG
XhoII 3 GGATCT [GA]^GATC[CT]
2652 AGATCC
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
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AcsI AfaI AluI AlwI ApoI
Asp700I AspHI AvaII BbvI BciVI
BfaI BmyI BseRI BsiHKAI BsiYI
BslI BsmAI BsmI Bsp1286I BsrDI
BsrI BstAPI BstF5I Cac8I Cfr13I
DdeI DpnII DraI Fnu4HI FokI
HaeIII HincII HindII HinfI HpaI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI RsaI Sau3AI Sau96I SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XmnI