Restriction map for
pF1KE2428
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BfrI
BlnI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT22I
FauI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 833 CGCG CG^CG
AcsI 1009 GAATTC [GA]^AATT[CT]
AlwI 681 GATCC GGATC(4/5)
AlwNI 883 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1009 GAATTC [GA]^AATT[CT]
BanI 276 GGTGCC G^G[CT][GA]CC
BbsI 127 GAAGAC GAAGAC(2/6)
BbvCI 820 GCTGAGG CCTCAGC(-5/-2)
BclI 782 TGATCA T^GATCA
BfaI 5 CTAG C^TAG
BlpI 44 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 44 GCTCAGC GC^T[ACGT]AGC
BpuAI 127 GAAGAC GAAGAC(2/6)
BsaWI 809 ACCGGA [AT]^CCGG[AT]
Bsp1407I 974 TGTACA T^GTACA
BsrGI 974 TGTACA T^GTACA
BstEII 105 GGTGACC G^GT[ACGT]ACC
BstPI 105 GGTGACC G^GT[ACGT]ACC
BstUI 833 CGCG CG^CG
CelII 44 GCTCAGC GC^T[ACGT]AGC
EaeI 620 TGGCCG [CT]^GGCC[GA]
EarI 721 CTCTTC CTCTTC(1/4)
EcoO65I 105 GGTGACC G^GT[ACGT]ACC
EcoRI 1009 GAATTC G^AATTC
EcoT14I 668 CCAAGG C^C[AT][AT]GG
FbaI 782 TGATCA T^GATCA
HaeII 413 AGCGCC [GA]GCGC^[CT]
Ksp632I 721 CTCTTC CTCTTC(1/4)
MaeI 5 CTAG C^TAG
MvnI 833 CGCG CG^CG
NdeI 1042 CATATG CA^TATG
NheI 4 GCTAGC G^CTAGC
PflMI 996 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 692 GACTC GAGTC(4/5)
PpuMI 506 AGGACCC [GA]G^G[AT]CC[CT]
SapI 720 GCTCTTC GCTCTTC(1/4)
SmaI 908 CCCGGG CCC^GGG
SmlI 313 CTCAAG C^T[CT][GA]AG
SspBI 974 TGTACA T^GTACA
StuI 824 AGGCCT AGG^CCT
StyI 668 CCAAGG C^C[AT][AT]GG
TfiI 1029 GAATC G^A[AT]TC
Tsp509I 1010 AATT ^AATT
Van91I 996 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 848 CCATTGAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 908 CCCGGG C^CCGGG
XmaI 908 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 364 GTAGAC GT^[AC][GT]AC
946 GTAGAC
AciI 673 GCGG CCGC(-3/-1)
804 GCGG
AfaI 836 GTAC GT^AC
975 GTAC
ApaI 372 GGGCCC GGGCC^C
895 GGGCCC
AvaI 898 CCCGAG C^[CT]CG[GA]G
908 CCCGGG
AvaII 237 GGTCC G^G[AT]CC
507 GGACC
BglI 79 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
622 GCCGTGGGGGC
BglII 299 AGATCT A^GATCT
593 AGATCT
BpmI 928 CTCCAG CTGGAG(16/14)
859 CTGGAG
BsmAI 487 GAGAC GTCTC(1/5)
901 GAGAC
BsoBI 898 CCCGAG C^[CT]CG[GA]G
908 CCCGGG
BspMI 188 ACCTGC ACCTGC(4/8)
790 ACCTGC
BsrDI 453 CATTGC GCAATG(2/0)
71 GCAATG
BstYI 299 AGATCT [GA]^GATC[CT]
593 AGATCT
CfoI 414 GCGC GCG^C
832 GCGC
DsaI 100 CCACGG C^C[GA][CT]GG
623 CCGTGG
HhaI 414 GCGC GCG^C
832 GCGC
HinP1I 414 GCGC G^CGC
832 GCGC
HinfI 692 GACTC G^A[ACGT]TC
1029 GAATC
HphI 105 GGTGA GGTGA(8/7)
1006 GGTGA
MaeIII 106 GTGAC ^GT[ACGT]AC
305 GTGAC
MboII 127 GAAGA GAAGA(8/7)
722 TCTTC
MflI 299 AGATCT [GA]^GATC[CT]
593 AGATCT
MseI 356 TTAA T^TAA
607 TTAA
MspA1I 82 CAGCTG C[AC]G^C[GT]G
856 CAGCTG
PvuII 82 CAGCTG CAG^CTG
856 CAGCTG
RsaI 836 GTAC GT^AC
975 GTAC
Tru9I 356 TTAA T^TAA
607 TTAA
Tsp45I 106 GTGAC ^GT[GC]AC
305 GTGAC
XhoII 299 AGATCT [GA]^GATC[CT]
593 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AluI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HpaII ItaI MboI
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI TspRI