Restriction map for
pF1KB6659
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MroI
MslI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I Van91I XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 343 CGCG CG^CG
AcyI 706 GGCGTC G[GA]^CG[CT]C
AlwNI 985 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 215 TGGCCA TGG^CCA
BbsI 1129 GAAGAC GAAGAC(2/6)
BbvCI 454 GCTGAGG CCTCAGC(-5/-2)
BcnI 123 CCGGG CC^[GC]GG
BglI 328 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1129 GAAGAC GAAGAC(2/6)
BsaHI 706 GGCGTC G[GA]^CG[CT]C
BsaI 946 GAGACC GGTCTC(1/5)
BsmBI 867 GAGACG CGTCTC(1/5)
BspHI 713 TCATGA T^CATGA
BsrDI 203 GCAATG GCAATG(2/0)
BstUI 343 CGCG CG^CG
CfoI 81 GCGC GCG^C
DraIII 151 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 215 TGGCCA [CT]^GGCC[GA]
FauI 185 GCGGG CCCGC(4/6)
HaeII 80 AGCGCC [GA]GCGC^[CT]
HapII 123 CCGG C^CGG
HhaI 81 GCGC GCG^C
Hin1I 706 GGCGTC G[GA]^CG[CT]C
HinP1I 81 GCGC G^CGC
HindIII 18 AAGCTT A^AGCTT
HpaII 123 CCGG C^CGG
HphI 156 GGTGA GGTGA(8/7)
MluNI 215 TGGCCA TGG^CCA
MscI 215 TGGCCA TGG^CCA
MspI 123 CCGG C^CGG
MvnI 343 CGCG CG^CG
NciI 123 CCGGG CC^[GC]GG
NruI 342 TCGCGA TCG^CGA
NspI 74 GCATGC [GA]CATG^[CT]
PpuMI 188 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 713 TCATGA T^CATGA
SapI 373 GAAGAGC GCTCTTC(1/4)
SmlI 886 CTTGAG C^T[CT][GA]AG
SphI 74 GCATGC GCATG^C
Tsp45I 814 GTCAC ^GT[GC]AC
XbaI 939 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 65 GTAC GT^AC
315 GTAC
AvaI 85 CTCGGG C^[CT]CG[GA]G
330 CCCGAG
AvaII 189 GGACC G^G[AT]CC
266 GGACC
BanII 36 GAGCTC G[GA]GC[CT]^C
996 GAGCTC
BcgI 345 CGACTACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
408 GCAAAATGCTCG
BsoBI 85 CTCGGG C^[CT]CG[GA]G
330 CCCGAG
DpnII 172 GATC ^GATC
514 GATC
DraII 50 AGGCCCC [GA]G^G[ACGT]CC[CT]
188 GGGACCT
EarI 373 GAAGAG CTCTTC(1/4)
1168 GAAGAG
EcoO109I 50 AGGCCCC [GA]G^G[ACGT]CC[CT]
188 GGGACCT
HgaI 869 GACGC GACGC(5/10)
707 GCGTC
Ksp632I 373 GAAGAG CTCTTC(1/4)
1168 GAAGAG
MaeII 495 ACGT A^CGT
1133 ACGT
MboI 172 GATC ^GATC
514 GATC
NdeII 172 GATC ^GATC
514 GATC
PleI 1080 GACTC GAGTC(4/5)
922 GAGTC
PstI 794 CTGCAG CTGCA^G
802 CTGCAG
RsaI 65 GTAC GT^AC
315 GTAC
SacI 36 GAGCTC GAGCT^C
996 GAGCTC
Sau3AI 172 GATC ^GATC
514 GATC
StuI 522 AGGCCT AGG^CCT
693 AGGCCT
TaqI 635 TCGA T^CGA
1083 TCGA
TfiI 573 GATTC G^A[AT]TC
1023 GATTC
TthHB8I 635 TCGA T^CGA
1083 TCGA
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AspHI BbvI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EcoNI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MaeIII MboII MnlI MseI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI SfaNI SfcI StyI Tru9I
TseI Tsp509I TspRI