Restriction map for
pF1KB5992
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp718I AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspEI
BspLU11I BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DraIII
DrdI DsaI EagI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MluI MroI MspI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AhdI 877 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 425 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1629 AGCGCT AGC^GCT
AspEI 877 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 799 GAGCAC G[AT]GC[AT]^C
AspI 1326 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 692 GGACC G^G[AT]CC
BbvCI 1087 CCTCAGC CCTCAGC(-5/-2)
BciVI 1126 GGATAC GTATCC(6/5)
BmyI 799 GAGCAC G[AGT]GC[ACT]^C
BsaBI 1341 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 812 GAGGAG GAGGAG(10/8)
BsiHKAI 799 GAGCAC G[AT]GC[AT]^C
Bsp1286I 799 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 792 TGTACA T^GTACA
BspDI 402 ATCGAT AT^CGAT
BspMI 273 ACCTGC ACCTGC(4/8)
BsrBI 1424 GAGCGG CCGCTC(-3/-3)
BsrDI 1713 CATTGC GCAATG(2/0)
BsrGI 792 TGTACA T^GTACA
BssSI 1808 CTCGTG CACGAG(-5/-1)
BstAPI 208 GCATTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1352 CCAGAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1605 GGATCT [GA]^GATC[CT]
CfoI 1630 GCGC GCG^C
ClaI 402 ATCGAT AT^CGAT
DraII 691 GGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 877 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1629 AGCGCT AGC^GCT
EcoO109I 691 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 1749 CCAAGG C^C[AT][AT]GG
HaeII 1629 AGCGCT [GA]GCGC^[CT]
HhaI 1630 GCGC GCG^C
HinP1I 1630 GCGC G^CGC
MaeII 927 ACGT A^CGT
MamI 1341 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 218 CAATTG C^AATTG
MflI 1605 GGATCT [GA]^GATC[CT]
MspA1I 1321 CAGCTG C[AC]G^C[GT]G
MunI 218 CAATTG C^AATTG
PflFI 1326 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1224 CCAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 691 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 1321 CAGCTG CAG^CTG
SmlI 1702 CTTGAG C^T[CT][GA]AG
SpeI 771 ACTAGT A^CTAGT
SspBI 792 TGTACA T^GTACA
StuI 1728 AGGCCT AGG^CCT
StyI 1749 CCAAGG C^C[AT][AT]GG
TaqI 403 TCGA T^CGA
Tsp45I 433 GTCAC ^GT[GC]AC
Tth111I 1326 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 403 TCGA T^CGA
Van91I 1224 CCAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1605 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 1247 GCGG CCGC(-3/-1)
1426 GCGG
AlwI 405 GATCC GGATC(4/5)
1605 GGATC
BalI 1343 TGGCCA TGG^CCA
1491 TGGCCA
BfaI 772 CTAG C^TAG
1264 CTAG
BsaJI 1482 CCCTGG C^C[ACGT][ACGT]GG
1749 CCAAGG
BsgI 1500 CTGCAC GTGCAG(16/14)
671 GTGCAG
BsmAI 466 GAGAC GTCTC(1/5)
1099 GTCTC
BspHI 473 TCATGA T^CATGA
1065 TCATGA
Cac8I 547 GCTCGC GC[ACGT]^[ACGT]GC
1092 GCTTGC
Cfr13I 692 GGACC G^G[ACGT]CC
786 GGCCC
DpnII 405 GATC ^GATC
1606 GATC
EaeI 1343 TGGCCA [CT]^GGCC[GA]
1491 TGGCCA
EcoT22I 206 ATGCAT ATGCA^T
665 ATGCAT
HphI 198 GGTGA GGTGA(8/7)
1349 TCACC
MaeI 772 CTAG C^TAG
1264 CTAG
MboI 405 GATC ^GATC
1606 GATC
MluNI 1343 TGGCCA TGG^CCA
1491 TGGCCA
MscI 1343 TGGCCA TGG^CCA
1491 TGGCCA
NdeII 405 GATC ^GATC
1606 GATC
NlaIV 691 GGGACC GG[ACGT]^[ACGT]CC
692 GGACCC
NsiI 206 ATGCAT ATGCA^T
665 ATGCAT
NspI 663 ACATGC [GA]CATG^[CT]
667 GCATGT
PleI 1470 GACTC GAGTC(4/5)
729 GAGTC
RcaI 473 TCATGA T^CATGA
1065 TCATGA
Sau3AI 405 GATC ^GATC
1606 GATC
Sau96I 692 GGACC G^G[ACGT]CC
786 GGCCC
SspI 1070 AATATT AAT^ATT
1271 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BbsI BbvI BpmI BpuAI BsiYI
BslI BsmFI BsmI BsrI BssKI
BstF5I BstNI DdeI EarI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeIII MboII MnlI MseI
MslI MvaI MwoI NdeI NlaIII
PstI RsaI ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp509I TspRI
XmnI