##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AgeI        AhdI        AocI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BanII       BbrPI       
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DsaI        Eam1105I    Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoT22I     FbaI        
FokI        FseI        FspI        HincII      HindII      
HindIII     HinfI       HpaI        KpnI        KspI        
MaeI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfuI        SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tru9I       Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII         150  GACGTC               GACGT^C
AfaI          369  GTAC                 GT^AC
AflIII        636  ACGTGT               A^C[GA][CT]GT
AluI          119  AGCT                 AG^CT
Alw44I        514  GTGCAC               G^TGCAC
AlwI           87  GGATC                GGATC(4/5)
ApaLI         514  GTGCAC               G^TGCAC
AspHI         514  GTGCAC               G[AT]GC[AT]^C
AvaI          267  CTCGGG               C^[CT]CG[GA]G
BmyI          514  GTGCAC               G[AGT]GC[ACT]^C
BsiEI         239  CGGCCG               CG[GA][CT]^CG
BsiHKAI       514  GTGCAC               G[AT]GC[AT]^C
BsoBI         267  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      514  GTGCAC               G[AGT]GC[ACT]^C
DpnII          88  GATC                 ^GATC
DraII         581  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DraIII        379  CACTTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          286  GACACCTTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          239  CGGCCG               C^GGCCG
EarI          169  CTCTTC               CTCTTC(1/4)
EclXI         239  CGGCCG               C^GGCCG
Eco52I        239  CGGCCG               C^GGCCG
EcoO109I      581  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRV         544  GATATC               GAT^ATC
FauI          502  GCGGG                CCCGC(4/6)
Ksp632I       169  CTCTTC               CTCTTC(1/4)
MboI           88  GATC                 ^GATC
NaeI          491  GCCGGC               GCC^GGC
NdeII          88  GATC                 ^GATC
NgoMIV        491  GCCGGC               G^CCGGC
NlaIII        366  CATG                 CATG^
PpuMI         581  GGGTCCT              [GA]G^G[AT]CC[CT]
RsaI          369  GTAC                 GT^AC
SapI          168  GCTCTTC              GCTCTTC(1/4)
Sau3AI         88  GATC                 ^GATC
SgrAI         440  CACCGGCG             C[GA]^CCGG[CT]G


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AlwNI         130  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              463  CAGCCCCTG           
AvaII          92  GGACC                G^G[AT]CC
              582  GGTCC               
BbsI          632  GAAGAC               GAAGAC(2/6)
              391  GTCTTC              
BpuAI         632  GAAGAC               GAAGAC(2/6)
              391  GTCTTC              
BsmAI         109  GAGAC                GTCTC(1/5)
              674  GAGAC               
BssSI         519  CACGAG               CACGAG(-5/-1)
              162  CTCGTG              
DdeI          304  CTGAG                C^T[ACGT]AG
              352  CTGAG               
EcoT14I       233  CCAAGG               C^C[AT][AT]GG
              563  CCTTGG              
HgaI          417  GACGC                GACGC(5/10)
              457  GCGTC               
HphI          299  TCACC                GGTGA(8/7)
              605  TCACC               
MaeIII         13  GTGAC                ^GT[ACGT]AC
              594  GTCAC               
StyI          233  CCAAGG               C^C[AT][AT]GG
              563  CCTTGG              
Tsp45I         13  GTGAC                ^GT[GC]AC
              594  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AccII       AciI        AcyI        BanI        BbeI        
BbvI        BcnI        BsaHI       BsaJI       BsiYI       
BslI        BsmFI       BsrFI       BsrI        BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      EaeI        EcoRII      Fnu4HI      HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HpaII       ItaI        KasI        MaeII       MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NlaIV       Sau96I      
ScrFI       SfoI        TseI        TspRI