Restriction map for
pF1KE2477
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Aor51HI ApaI AseI AsnI
Asp718I AspEI AspI AvaII AviII
AvrII BbvCI BcgI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspLU11I BspMI Bst1107I BstAPI
BstBI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI FspI HgaI
HpaI KpnI KspI MamI MfeI
MroI MspA1I MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI SexAI SfiI SfuI SmlI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccI 1735 GTCTAC GT^[AC][GT]AC
AcsI 516 GAATTC [GA]^AATT[CT]
AocI 1702 CCTAAGG CC^T[ACGT]AGG
ApoI 516 GAATTC [GA]^AATT[CT]
AscI 120 GGCGCGCC GG^CGCGCC
Asp700I 354 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1480 CCCGGG C^[CT]CG[GA]G
BalI 523 TGGCCA TGG^CCA
BamHI 1511 GGATCC G^GATCC
BanII 1117 GGGCTC G[GA]GC[CT]^C
BbeI 58 GGCGCC GGCGC^C
BbrPI 988 CACGTG CAC^GTG
BciVI 148 GGATAC GTATCC(6/5)
BfaI 1054 CTAG C^TAG
BsaWI 344 ACCGGA [AT]^CCGG[AT]
BsgI 1007 GTGCAG GTGCAG(16/14)
BsmBI 1625 GAGACG CGTCTC(1/5)
BsoBI 1480 CCCGGG C^[CT]CG[GA]G
BsrBI 292 GAGCGG CCGCTC(-3/-3)
BsrDI 1029 CATTGC GCAATG(2/0)
BsrFI 1504 GCCGGC [GA]^CCGG[CT]
BssHII 121 GCGCGC G^CGCGC
BssSI 1297 CACGAG CACGAG(-5/-1)
BstEII 1371 GGTCACC G^GT[ACGT]ACC
BstPI 1371 GGTCACC G^GT[ACGT]ACC
Bsu36I 1702 CCTAAGG CC^T[ACGT]AGG
Cfr10I 1504 GCCGGC [GA]^CCGG[CT]
DraII 460 AGGCCCC [GA]G^G[ACGT]CC[CT]
EarI 358 CTCTTC CTCTTC(1/4)
Eco81I 1702 CCTAAGG CC^T[ACGT]AGG
EcoNI 1194 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 460 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1371 GGTCACC G^GT[ACGT]ACC
EcoRI 516 GAATTC G^AATTC
HincII 1186 GTCAAC GT[CT]^[GA]AC
HindII 1186 GTCAAC GT[CT]^[GA]AC
HindIII 976 AAGCTT A^AGCTT
KasI 58 GGCGCC G^GCGCC
Ksp632I 358 CTCTTC CTCTTC(1/4)
MaeI 1054 CTAG C^TAG
MluI 1664 ACGCGT A^CGCGT
MluNI 523 TGGCCA TGG^CCA
MscI 523 TGGCCA TGG^CCA
NaeI 1504 GCCGGC GCC^GGC
NarI 58 GGCGCC GG^CGCC
NgoMIV 1504 GCCGGC G^CCGGC
PmaCI 988 CACGTG CAC^GTG
PmlI 988 CACGTG CAC^GTG
PshAI 490 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 211 CTGCAG CTGCA^G
SapI 357 GCTCTTC GCTCTTC(1/4)
ScaI 890 AGTACT AGT^ACT
SfcI 211 CTGCAG C^T[GA][CT]AG
SfoI 58 GGCGCC GGC^GCC
SgrAI 1503 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1480 CCCGGG CCC^GGG
SphI 640 GCATGC GCATG^C
SspI 535 AATATT AAT^ATT
TaqI 1658 TCGA T^CGA
TthHB8I 1658 TCGA T^CGA
XcmI 102 CCACTCCAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1480 CCCGGG C^CCGGG
XmaI 1480 CCCGGG C^CCGGG
XmnI 354 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 259 GACGTC GACGT^C
685 GACGTC
Alw44I 751 GTGCAC G^TGCAC
1347 GTGCAC
AlwNI 1270 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
1579 CAGCATCTG
ApaLI 751 GTGCAC G^TGCAC
1347 GTGCAC
AspHI 751 GTGCAC G[AT]GC[AT]^C
1347 GTGCAC
BbsI 529 GAAGAC GAAGAC(2/6)
838 GAAGAC
BglII 1094 AGATCT A^GATCT
1532 AGATCT
BpmI 1742 CTCCAG CTGGAG(16/14)
1195 CTGGAG
BpuAI 529 GAAGAC GAAGAC(2/6)
838 GAAGAC
BsaAI 988 CACGTG [CT]AC^GT[GA]
1471 TACGTG
BsaI 584 GAGACC GGTCTC(1/5)
716 GAGACC
BsiHKAI 751 GTGCAC G[AT]GC[AT]^C
1347 GTGCAC
BspHI 329 TCATGA T^CATGA
486 TCATGA
EaeI 40 TGGCCG [CT]^GGCC[GA]
523 TGGCCA
HaeII 58 GGCGCC [GA]GCGC^[CT]
1301 AGCGCC
MseI 539 TTAA T^TAA
1412 TTAA
MslI 324 CATTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
609 CATCCCTGTG
NspI 640 GCATGC [GA]CATG^[CT]
896 GCATGT
RcaI 329 TCATGA T^CATGA
486 TCATGA
Tru9I 539 TTAA T^TAA
1412 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI BanI BbvI BcnI
BmyI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SspBI StyI TfiI
TseI Tsp45I Tsp509I TspRI XhoII