Restriction map for
pF1KE2751
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AflII AgeI AhdI AlwI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanII BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstNI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DpnII DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRI EcoRII FauI FbaI
FseI FspI HaeIII HgaI HincII
HindII HindIII HpaI HphI KpnI
KspI MaeI MamI MboI MflI
MluI MluNI MroI MscI MseI
MslI MspA1I MvaI MvnI NaeI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tru9I Tsp45I
Tth111I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AclI 106 AACGTT AA^CGTT
AcsI 324 AAATTT [GA]^AATT[CT]
AcyI 83 GGCGCC G[GA]^CG[CT]C
AflIII 493 ACATGT A^C[GA][CT]GT
AluI 297 AGCT AG^CT
Alw44I 436 GTGCAC G^TGCAC
ApaLI 436 GTGCAC G^TGCAC
ApoI 324 AAATTT [GA]^AATT[CT]
AspHI 436 GTGCAC G[AT]GC[AT]^C
AvaI 74 CCCGGG C^[CT]CG[GA]G
AvaII 525 GGTCC G^G[AT]CC
BanI 83 GGCGCC G^G[CT][GA]CC
BbeI 83 GGCGCC GGCGC^C
BglI 122 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 436 GTGCAC G[AGT]GC[ACT]^C
BsaHI 83 GGCGCC G[GA]^CG[CT]C
BsgI 330 CTGCAC GTGCAG(16/14)
BsiHKAI 436 GTGCAC G[AT]GC[AT]^C
BsmI 95 GAATGC GAATGC(1/-1)
BsoBI 74 CCCGGG C^[CT]CG[GA]G
Bsp1286I 436 GTGCAC G[AGT]GC[ACT]^C
BspLU11I 493 ACATGT A^CATGT
BstEII 559 GGTAACC G^GT[ACGT]ACC
BstPI 559 GGTAACC G^GT[ACGT]ACC
Cac8I 86 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 84 GCGC GCG^C
Cfr13I 525 GGTCC G^G[ACGT]CC
DdeI 144 CTCAG C^T[ACGT]AG
EarI 231 CTCTTC CTCTTC(1/4)
EcoNI 667 CCTAATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 559 GGTAACC G^GT[ACGT]ACC
EcoRV 263 GATATC GAT^ATC
EcoT22I 97 ATGCAT ATGCA^T
HaeII 83 GGCGCC [GA]GCGC^[CT]
HapII 75 CCGG C^CGG
HhaI 84 GCGC GCG^C
Hin1I 83 GGCGCC G[GA]^CG[CT]C
HinP1I 84 GCGC G^CGC
HpaII 75 CCGG C^CGG
KasI 83 GGCGCC G^GCGCC
Ksp632I 231 CTCTTC CTCTTC(1/4)
MaeII 107 ACGT A^CGT
MaeIII 560 GTAAC ^GT[ACGT]AC
MfeI 381 CAATTG C^AATTG
MspI 75 CCGG C^CGG
MunI 381 CAATTG C^AATTG
NarI 83 GGCGCC GG^CGCC
NsiI 97 ATGCAT ATGCA^T
PflMI 411 CCATACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 468 GAGTC GAGTC(4/5)
Psp1406I 106 AACGTT AA^CGTT
Sau96I 525 GGTCC G^G[ACGT]CC
SfoI 83 GGCGCC GGC^GCC
SmaI 74 CCCGGG CCC^GGG
TaqI 261 TCGA T^CGA
TthHB8I 261 TCGA T^CGA
Van91I 411 CCATACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 74 CCCGGG C^CCGGG
XmaI 74 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
BcnI 74 CCCGG CC^[GC]GG
75 CCGGG
BsmAI 583 GAGAC GTCTC(1/5)
227 GTCTC
BsmFI 511 GGGAC GGGAC(10/14)
569 GTCCC
BssKI 74 CCCGG ^CC[ACGT]GG
75 CCGGG
NciI 74 CCCGG CC^[GC]GG
75 CCGGG
NlaIV 83 GGCGCC GG[ACGT]^[ACGT]CC
524 GGGTCC
NspI 139 ACATGC [GA]CATG^[CT]
493 ACATGT
ScrFI 74 CCCGG CC^[ACGT]GG
75 CCGGG
SfaNI 99 GCATC GCATC(5/9)
543 GCATC
TfiI 459 GAATC G^A[AT]TC
715 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AfaI BbvI BsaJI BsiYI BslI
Bsp1407I BsrGI BstF5I EcoT14I Fnu4HI
FokI HinfI ItaI MboII MnlI
MwoI NlaIII RsaI SspBI StyI
TseI Tsp509I TspRI