Restriction map for
pF1KE0131
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BanII BbrPI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HindIII HpaI KpnI KspI
MaeIII MfeI MluI MluNI MroI
MscI MseI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp45I Tth111I TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccII 445 CGCG CG^CG
AciI 28 GCGG CCGC(-3/-1)
AlwI 121 GGATC GGATC(4/5)
AspHI 88 GAGCAC G[AT]GC[AT]^C
AvaI 131 CCCGGG C^[CT]CG[GA]G
BbsI 413 GAAGAC GAAGAC(2/6)
BclI 491 TGATCA T^GATCA
BfaI 371 CTAG C^TAG
BmyI 88 GAGCAC G[AGT]GC[ACT]^C
BpuAI 413 GAAGAC GAAGAC(2/6)
BsaBI 283 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 484 GAGACC GGTCTC(1/5)
BsiHKAI 88 GAGCAC G[AT]GC[AT]^C
BsmAI 484 GAGAC GTCTC(1/5)
BsmI 257 GAATGC GAATGC(1/-1)
BsoBI 131 CCCGGG C^[CT]CG[GA]G
Bsp1286I 88 GAGCAC G[AGT]GC[ACT]^C
BspHI 407 TCATGA T^CATGA
BsrDI 519 CATTGC GCAATG(2/0)
BsrFI 434 ACCGGC [GA]^CCGG[CT]
BsrI 393 ACTGG ACTGG(1/-1)
BssHII 444 GCGCGC G^CGCGC
BstUI 445 CGCG CG^CG
BstXI 224 CCAAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 121 GGATCT [GA]^GATC[CT]
Cfr10I 434 ACCGGC [GA]^CCGG[CT]
DdeI 262 CTGAG C^T[ACGT]AG
DraIII 91 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 276 CCATGG C^C[GA][CT]GG
EaeI 512 CGGCCA [CT]^GGCC[GA]
EarI 379 GAAGAG CTCTTC(1/4)
FbaI 491 TGATCA T^GATCA
HincII 430 GTCAAC GT[CT]^[GA]AC
HindII 430 GTCAAC GT[CT]^[GA]AC
HphI 96 GGTGA GGTGA(8/7)
Ksp632I 379 GAAGAG CTCTTC(1/4)
MaeI 371 CTAG C^TAG
MaeII 324 ACGT A^CGT
MamI 283 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 121 GGATCT [GA]^GATC[CT]
MvnI 445 CGCG CG^CG
NcoI 276 CCATGG C^CATGG
PflMI 524 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 235 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 407 TCATGA T^CATGA
SexAI 92 ACCTGGT A^CC[AT]GGT
SmaI 131 CCCGGG CCC^GGG
Tsp509I 310 AATT ^AATT
TspRI 359 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 524 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 121 GGATCT [GA]^GATC[CT]
XmaCI 131 CCCGGG C^CCGGG
XmaI 131 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 300 GTAC GT^AC
391 GTAC
AvaII 128 GGTCC G^G[AT]CC
236 GGTCC
BbeI 463 GGCGCC GGCGC^C
473 GGCGCC
BbvI 172 GCTGC GCAGC(8/12)
243 GCTGC
BcnI 131 CCCGG CC^[GC]GG
132 CCGGG
BsiYI 288 CCATCTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
524 CCAACCTGTGG
BslI 288 CCATCTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
524 CCAACCTGTGG
BstF5I 282 GGATG GGATG(2/0)
539 GGATG
DraII 9 AGGCCCT [GA]G^G[ACGT]CC[CT]
235 GGGTCCC
EcoO109I 9 AGGCCCT [GA]G^G[ACGT]CC[CT]
235 GGGTCCC
Fnu4HI 172 GCTGC GC^[ACGT]GC
243 GCTGC
FokI 282 GGATG GGATG(9/13)
539 GGATG
HaeII 463 GGCGCC [GA]GCGC^[CT]
473 GGCGCC
HapII 132 CCGG C^CGG
435 CCGG
HgaI 68 GACGC GACGC(5/10)
438 GCGTC
HinfI 60 GAATC G^A[ACGT]TC
268 GAATC
HpaII 132 CCGG C^CGG
435 CCGG
ItaI 172 GCTGC GC^[ACGT]GC
243 GCTGC
KasI 463 GGCGCC G^GCGCC
473 GGCGCC
MslI 277 CATGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
495 CATCGCCGTG
MspA1I 241 CAGCTG C[AC]G^C[GT]G
418 CAGCTG
MspI 132 CCGG C^CGG
435 CCGG
NarI 463 GGCGCC GG^CGCC
473 GGCGCC
NciI 131 CCCGG CC^[GC]GG
132 CCGGG
PvuII 241 CAGCTG CAG^CTG
418 CAGCTG
RsaI 300 GTAC GT^AC
391 GTAC
SfaNI 283 GATGC GCATC(5/9)
355 GATGC
SfoI 463 GGCGCC GGC^GCC
473 GGCGCC
TfiI 60 GAATC G^A[AT]TC
268 GAATC
TseI 172 GCTGC G^C[AT]GC
243 GCTGC
XcmI 23 CCAAGGCGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
294 CCAGGAGTACGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcyI AluI BanI BpmI BsaHI
BsaJI BsmFI BssKI BstNI Cac8I
CfoI Cfr13I DpnII EcoRII EcoT14I
HaeIII HhaI Hin1I HinP1I MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
StyI