Restriction map for
pF1KB4585
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MslI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 982 GTCTAC GT^[AC][GT]AC
AccII 723 CGCG CG^CG
AcyI 513 GACGCC G[GA]^CG[CT]C
AflIII 287 ACGTGT A^C[GA][CT]GT
AlwI 499 GATCC GGATC(4/5)
AspHI 111 GAGCTC G[AT]GC[AT]^C
AvaI 194 CCCGAG C^[CT]CG[GA]G
BcgI 269 GCACAGTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 103 GTATCC GTATCC(6/5)
BglI 574 GCCACACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 513 GACGCC G[GA]^CG[CT]C
BsiHKAI 111 GAGCTC G[AT]GC[AT]^C
BsoBI 194 CCCGAG C^[CT]CG[GA]G
BspHI 482 TCATGA T^CATGA
BspMI 298 ACCTGC ACCTGC(4/8)
BsrI 857 CCAGT ACTGG(1/-1)
BstEII 294 GGTCACC G^GT[ACGT]ACC
BstF5I 410 CATCC GGATG(2/0)
BstPI 294 GGTCACC G^GT[ACGT]ACC
BstUI 723 CGCG CG^CG
BstYI 498 AGATCC [GA]^GATC[CT]
CfoI 58 GCGC GCG^C
DrdI 556 GACAACACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 299 CCTGCAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 294 GGTCACC G^GT[ACGT]ACC
FokI 410 CATCC GGATG(9/13)
HaeII 57 GGCGCT [GA]GCGC^[CT]
HhaI 58 GCGC GCG^C
Hin1I 513 GACGCC G[GA]^CG[CT]C
HinP1I 58 GCGC G^CGC
HinfI 610 GACTC G^A[ACGT]TC
MaeII 287 ACGT A^CGT
MflI 498 AGATCC [GA]^GATC[CT]
MvnI 723 CGCG CG^CG
NaeI 65 GCCGGC GCC^GGC
NgoMIV 65 GCCGGC G^CCGGC
PleI 610 GACTC GAGTC(4/5)
PpuMI 683 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 482 TCATGA T^CATGA
SacI 111 GAGCTC GAGCT^C
ScaI 221 AGTACT AGT^ACT
SexAI 236 ACCTGGT A^CC[AT]GGT
SfaNI 999 GATGC GCATC(5/9)
SfiI 573 GGCCACACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 278 TCGA T^CGA
Tsp509I 165 AATT ^AATT
TthHB8I 278 TCGA T^CGA
XcmI 413 CCAATGGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 498 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 684 GGACC G^G[AT]CC
777 GGTCC
BcnI 441 CCGGG CC^[GC]GG
1014 CCCGG
BsmAI 170 GAGAC GTCTC(1/5)
628 GAGAC
BsrFI 65 GCCGGC [GA]^CCGG[CT]
736 GCCGGT
Cfr10I 65 GCCGGC [GA]^CCGG[CT]
736 GCCGGT
DraII 581 AGGCCCC [GA]G^G[ACGT]CC[CT]
683 GGGACCC
EarI 643 GAAGAG CTCTTC(1/4)
648 GAAGAG
EcoO109I 581 AGGCCCC [GA]G^G[ACGT]CC[CT]
683 GGGACCC
EcoT14I 707 CCAAGG C^C[AT][AT]GG
863 CCAAGG
HgaI 513 GACGC GACGC(5/10)
743 GCGTC
Ksp632I 643 GAAGAG CTCTTC(1/4)
648 GAAGAG
NciI 441 CCGGG CC^[GC]GG
1014 CCCGG
PvuII 257 CAGCTG CAG^CTG
523 CAGCTG
SfcI 264 CTGTAG C^T[GA][CT]AG
923 CTACAG
StyI 707 CCAAGG C^C[AT][AT]GG
863 CCAAGG
TspRI 561 CACTG CA[GC]TG[ACGT][ACGT]^
873 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI BalI BanI
BanII BbvI BmyI BpmI BsaJI
BsiYI BslI BsmFI Bsp1286I BssKI
BstNI BstXI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII FauI Fnu4HI
HaeIII HapII HpaII HphI ItaI
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI TseI Tsp45I