##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DpnII       DraI        
DraII       DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoT14I     FbaI        
FseI        FspI        HaeII       HapII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeII       MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspI        MunI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIV       NotI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AfaI          663  GTAC                 GT^AC
AlwNI         215  CAGTAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         510  GGACC                G^G[AT]CC
BciVI         145  GGATAC               GTATCC(6/5)
BpmI          284  CTGGAG               CTGGAG(16/14)
BsmFI         450  GTCCC                GGGAC(10/14)
BsmI          256  GAATGC               GAATGC(1/-1)
BspMI         117  ACCTGC               ACCTGC(4/8)
BsrBI          40  GAGCGG               CCGCTC(-3/-3)
BssKI         404  CCAGG                ^CC[ACGT]GG
BstEII        174  GGTGACC              G^GT[ACGT]ACC
BstF5I        646  CATCC                GGATG(2/0)
BstNI         404  CCAGG                CC^[AT]GG
BstPI         174  GGTGACC              G^GT[ACGT]ACC
BstXI         276  CCATGATACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I          38  GCGAGC               GC[ACGT]^[ACGT]GC
Cfr13I        510  GGACC                G^G[ACGT]CC
DraIII         94  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I       174  GGTGACC              G^GT[ACGT]ACC
EcoRI         396  GAATTC               G^AATTC
EcoRII        404  CCAGG                ^CC[AT]GG
EcoRV         472  GATATC               GAT^ATC
EcoT22I       258  ATGCAT               ATGCA^T
FokI          646  CATCC                GGATG(9/13)
HaeIII        715  GGCC                 GG^CC
MvaI          404  CCAGG                CC^[AT]GG
NruI           36  TCGCGA               TCG^CGA
NsiI          258  ATGCAT               ATGCA^T
PvuII         748  CAGCTG               CAG^CTG
RsaI          663  GTAC                 GT^AC
Sau96I        510  GGACC                G^G[ACGT]CC
ScrFI         404  CCAGG                CC^[ACGT]GG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AccII          37  CGCG                 CG^CG
               80  CGCG                
AcsI          356  AAATTC               [GA]^AATT[CT]
              396  GAATTC              
AluI          498  AGCT                 AG^CT
              749  AGCT                
ApoI          356  AAATTC               [GA]^AATT[CT]
              396  GAATTC              
BsmAI         704  GAGAC                GTCTC(1/5)
              637  GTCTC               
BstUI          37  CGCG                 CG^CG
               80  CGCG                
FauI           13  GCGGG                CCCGC(4/6)
               42  GCGGG               
MspA1I         11  CAGCGG               C[AC]G^C[GT]G
              748  CAGCTG              
MvnI           37  CGCG                 CG^CG
               80  CGCG                
NlaIII        277  CATG                 CATG^
              654  CATG                
PleI          408  GACTC                GAGTC(4/5)
              586  GACTC               
SfaNI         682  GATGC                GCATC(5/9)
              457  GCATC               
SmlI           49  CTCAAG               C^T[CT][GA]AG
              151  CTTGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

AciI        BbvI        BfaI        BsrI        CfoI        
DdeI        Fnu4HI      HhaI        HinP1I      HinfI       
HphI        ItaI        MaeI        MaeIII      MboII       
MnlI        MseI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I