Restriction map for
pF1KB8923
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaII AvrII BalI BamHI BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HincII HindII
HindIII HpaI HphI KpnI Ksp632I
MaeI MamI MboII MfeI MluI
MluNI MscI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SalI SapI SbfI ScaI SexAI
SfcI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tsp45I TspRI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AlwNI 616 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 590 GGGCCC GGGCC^C
Asp700I 633 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 3 GAGCTC G[AT]GC[AT]^C
AvaI 174 CTCGGG C^[CT]CG[GA]G
BbvCI 286 CCTCAGC CCTCAGC(-5/-2)
BglII 524 AGATCT A^GATCT
BsgI 547 CTGCAC GTGCAG(16/14)
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
BsmAI 708 GAGAC GTCTC(1/5)
BsoBI 174 CTCGGG C^[CT]CG[GA]G
BspHI 558 TCATGA T^CATGA
BspMI 128 GCAGGT ACCTGC(4/8)
BsrBI 171 CCGCTC CCGCTC(-3/-3)
BsrI 57 CCAGT ACTGG(1/-1)
BssHII 204 GCGCGC G^CGCGC
BstF5I 536 GGATG GGATG(2/0)
FokI 536 GGATG GGATG(9/13)
HinfI 106 GACTC G^A[ACGT]TC
KspI 785 CCGCGG CCGC^GG
MaeII 271 ACGT A^CGT
MaeIII 653 GTTAC ^GT[ACGT]AC
MseI 716 TTAA T^TAA
PflMI 615 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 106 GACTC GAGTC(4/5)
RcaI 558 TCATGA T^CATGA
SacI 3 GAGCTC GAGCT^C
SacII 785 CCGCGG CCGC^GG
SfaNI 537 GATGC GCATC(5/9)
SfiI 591 GGCCCGGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 116 GCATGC GCATG^C
Tru9I 716 TTAA T^TAA
Van91I 615 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 633 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccIII 196 TCCGGA T^CCGGA
439 TCCGGA
AfaI 132 GTAC GT^AC
255 GTAC
AlwI 295 GATCC GGATC(4/5)
156 GGATC
AviII 483 TGCGCA TGC^GCA
539 TGCGCA
BseAI 196 TCCGGA T^CCGGA
439 TCCGGA
BseRI 284 CTCCTC GAGGAG(10/8)
448 GAGGAG
BsmFI 104 GGGAC GGGAC(10/14)
806 GTCCC
BspEI 196 TCCGGA T^CCGGA
439 TCCGGA
BsrFI 264 GCCGGC [GA]^CCGG[CT]
394 GCCGGC
BstYI 156 GGATCT [GA]^GATC[CT]
524 AGATCT
Cfr10I 264 GCCGGC [GA]^CCGG[CT]
394 GCCGGC
DraII 589 AGGGCCC [GA]G^G[ACGT]CC[CT]
796 GGGGCCT
EagI 168 CGGCCG C^GGCCG
386 CGGCCG
EclXI 168 CGGCCG C^GGCCG
386 CGGCCG
Eco52I 168 CGGCCG C^GGCCG
386 CGGCCG
EcoO109I 589 AGGGCCC [GA]G^G[ACGT]CC[CT]
796 GGGGCCT
FspI 483 TGCGCA TGC^GCA
539 TGCGCA
MflI 156 GGATCT [GA]^GATC[CT]
524 AGATCT
MroI 196 TCCGGA T^CCGGA
439 TCCGGA
MslI 64 CATAATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
774 CATGAGCATG
NaeI 264 GCCGGC GCC^GGC
394 GCCGGC
NgoMIV 264 GCCGGC G^CCGGC
394 GCCGGC
NspI 116 GCATGC [GA]CATG^[CT]
687 ACATGC
RsaI 132 GTAC GT^AC
255 GTAC
XhoII 156 GGATCT [GA]^GATC[CT]
524 AGATCT
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BanII BbeI BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BsaWI
BsiEI BsiYI BslI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI KasI MboI MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfoI TseI Tsp509I