Restriction map for
pF1KB6608
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AclI AflII AgeI
Alw44I AlwNI AocI ApaI ApaLI
AseI AsnI Asp700I Asp718I AspHI
AspI AvaI AvrII BbeI BbrPI
BbsI BbvCI BciVI BfaI BfrI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspHI
BsrBI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EagI EclXI Eco52I Eco81I EcoO65I
EcoRV EcoT22I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 17 CGCG CG^CG
AccIII 984 TCCGGA T^CCGGA
AcyI 1219 GGCGTC G[GA]^CG[CT]C
AhdI 1133 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 796 AGCGCT AGC^GCT
AscI 15 GGCGCGCC GG^CGCGCC
AspEI 1133 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 1132 GGACC G^G[AT]CC
BalI 504 TGGCCA TGG^CCA
BamHI 1253 GGATCC G^GATCC
BanI 596 GGCACC G^G[CT][GA]CC
BcgI 388 CGACCCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 656 TGATCA T^GATCA
BglI 7 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 322 AGATCT A^GATCT
BpmI 479 CTCCAG CTGGAG(16/14)
BsaAI 619 TACGTG [CT]AC^GT[GA]
BsaHI 1219 GGCGTC G[GA]^CG[CT]C
BseAI 984 TCCGGA T^CCGGA
BsgI 403 GTGCAG GTGCAG(16/14)
BsmFI 1131 GGGAC GGGAC(10/14)
BspEI 984 TCCGGA T^CCGGA
BspLU11I 112 ACATGT A^CATGT
BsrFI 263 GCCGGC [GA]^CCGG[CT]
BssHII 16 GCGCGC G^CGCGC
BssSI 1227 CACGAG CACGAG(-5/-1)
BstUI 17 CGCG CG^CG
Cfr10I 263 GCCGGC [GA]^CCGG[CT]
DrdI 700 GACTACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1133 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 796 AGCGCT AGC^GCT
EcoNI 780 CCTACACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 524 CCCGC CCCGC(4/6)
FbaI 656 TGATCA T^GATCA
FseI 262 GGCCGGCC GGCCGG^CC
Hin1I 1219 GGCGTC G[GA]^CG[CT]C
MluNI 504 TGGCCA TGG^CCA
MroI 984 TCCGGA T^CCGGA
MscI 504 TGGCCA TGG^CCA
MvnI 17 CGCG CG^CG
NaeI 263 GCCGGC GCC^GGC
NcoI 257 CCATGG C^CATGG
NgoMIV 263 GCCGGC G^CCGGC
NspI 112 ACATGT [GA]CATG^[CT]
PpuMI 1131 GGGACCC [GA]G^G[AT]CC[CT]
PstI 363 CTGCAG CTGCA^G
SapI 331 GCTCTTC GCTCTTC(1/4)
ScaI 128 AGTACT AGT^ACT
SfaNI 328 GATGC GCATC(5/9)
SmlI 723 CTCAAG C^T[CT][GA]AG
TfiI 1108 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 709 GTCTAC GT^[AC][GT]AC
1222 GTCTAC
AfaI 129 GTAC GT^AC
309 GTAC
AflIII 112 ACATGT A^C[GA][CT]GT
620 ACGTGT
AviII 451 TGCGCA TGC^GCA
864 TGCGCA
BanII 5 GAGCCC G[GA]GC[CT]^C
606 GGGCTC
BsaWI 380 ACCGGA [AT]^CCGG[AT]
984 TCCGGA
BseRI 471 CTCCTC GAGGAG(10/8)
476 CTCCTC
BsmAI 1121 GAGAC GTCTC(1/5)
1234 GTCTC
BsrDI 825 CATTGC GCAATG(2/0)
62 GCAATG
DdeI 517 CTGAG C^T[ACGT]AG
1125 CTCAG
DraII 520 AGGCCCC [GA]G^G[ACGT]CC[CT]
1131 GGGACCC
DsaI 257 CCATGG C^C[GA][CT]GG
1191 CCGTGG
EaeI 504 TGGCCA [CT]^GGCC[GA]
581 CGGCCA
EarI 332 CTCTTC CTCTTC(1/4)
1273 GAAGAG
EcoO109I 520 AGGCCCC [GA]G^G[ACGT]CC[CT]
1131 GGGACCC
EcoRI 547 GAATTC G^AATTC
963 GAATTC
FspI 451 TGCGCA TGC^GCA
864 TGCGCA
HaeII 661 AGCGCC [GA]GCGC^[CT]
796 AGCGCT
HgaI 32 GCGTC GACGC(5/10)
1220 GCGTC
Ksp632I 332 CTCTTC CTCTTC(1/4)
1273 GAAGAG
MaeII 307 ACGT A^CGT
620 ACGT
MslI 615 CACCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1068 CACCAAAATG
MspA1I 337 CAGCTG C[AC]G^C[GT]G
991 CAGCTG
PvuII 337 CAGCTG CAG^CTG
991 CAGCTG
RsaI 129 GTAC GT^AC
309 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfcI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII