Restriction map for
pF1KB5482
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# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BanII BbeI BbrPI
BcgI BclI BfaI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1286I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRII EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MaeI MaeII MamI MfeI MflI
MluI MroI MunI MvaI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 424 AAATTC [GA]^AATT[CT]
AflII 196 CTTAAG C^TTAAG
ApoI 424 AAATTC [GA]^AATT[CT]
AvaI 140 CCCGGG C^[CT]CG[GA]G
AvaII 662 GGACC G^G[AT]CC
BalI 602 TGGCCA TGG^CCA
BanI 271 GGCACC G^G[CT][GA]CC
BbsI 179 GAAGAC GAAGAC(2/6)
BbvCI 190 CCTCAGC CCTCAGC(-5/-2)
BciVI 385 GGATAC GTATCC(6/5)
BfrI 196 CTTAAG C^TTAAG
BglI 224 GCCGACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 179 GAAGAC GAAGAC(2/6)
BsgI 625 CTGCAC GTGCAG(16/14)
BsmAI 685 GTCTC GTCTC(1/5)
BsmFI 661 GGGAC GGGAC(10/14)
BsoBI 140 CCCGGG C^[CT]CG[GA]G
BspHI 536 TCATGA T^CATGA
BsrDI 370 GCAATG GCAATG(2/0)
Cfr13I 662 GGACC G^G[ACGT]CC
DraII 661 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 602 TGGCCA [CT]^GGCC[GA]
EcoO109I 661 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 342 CCAAGG C^C[AT][AT]GG
HapII 141 CCGG C^CGG
HpaII 141 CCGG C^CGG
MluNI 602 TGGCCA TGG^CCA
MscI 602 TGGCCA TGG^CCA
MspI 141 CCGG C^CGG
PpuMI 661 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 536 TCATGA T^CATGA
Sau96I 662 GGACC G^G[ACGT]CC
SmaI 140 CCCGGG CCC^GGG
SmlI 196 CTTAAG C^T[CT][GA]AG
StyI 342 CCAAGG C^C[AT][AT]GG
Tsp45I 457 GTGAC ^GT[GC]AC
XcmI 356 CCAAGCTGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 140 CCCGGG C^CCGGG
XmaI 140 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 49 CGCG CG^CG
55 CGCG
BcnI 140 CCCGG CC^[GC]GG
141 CCGGG
BsiYI 91 CCCAGCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
161 CCAAGAAGAGG
BslI 91 CCCAGCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
161 CCAAGAAGAGG
Bsp1407I 392 TGTACA T^GTACA
608 TGTACA
BsrGI 392 TGTACA T^GTACA
608 TGTACA
BssKI 140 CCCGG ^CC[ACGT]GG
141 CCGGG
BstUI 49 CGCG CG^CG
55 CGCG
CfoI 54 GCGC GCG^C
171 GCGC
DdeI 191 CTCAG C^T[ACGT]AG
416 CTGAG
FauI 47 CCCGC CCCGC(4/6)
659 GCGGG
HaeIII 496 GGCC GG^CC
603 GGCC
HhaI 54 GCGC GCG^C
171 GCGC
HinP1I 54 GCGC G^CGC
171 GCGC
HinfI 433 GAATC G^A[ACGT]TC
727 GAATC
MaeIII 208 GTTAC ^GT[ACGT]AC
457 GTGAC
MslI 312 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
413 CACCTGAGTG
MspA1I 108 CAGCTG C[AC]G^C[GT]G
244 CAGCTG
MvnI 49 CGCG CG^CG
55 CGCG
NciI 140 CCCGG CC^[GC]GG
141 CCGGG
PstI 247 CTGCAG CTGCA^G
569 CTGCAG
PvuII 108 CAGCTG CAG^CTG
244 CAGCTG
ScrFI 140 CCCGG CC^[ACGT]GG
141 CCGGG
SfcI 247 CTGCAG C^T[GA][CT]AG
569 CTGCAG
SspBI 392 TGTACA T^GTACA
608 TGTACA
TfiI 433 GAATC G^A[AT]TC
727 GAATC
Tsp509I 425 AATT ^AATT
448 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BstF5I Cac8I DpnII EarI Fnu4HI
FokI ItaI Ksp632I MboI MboII
MnlI MseI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Tru9I TseI
TspRI