Restriction map for
pF1KB3865
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvrII BamHI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BseRI BsiWI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BssSI Bst1107I BstAPI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
MamI MfeI MluI MroI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuII RcaI
RsrII SalI SbfI SexAI SfiI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 571 GTCTAC GT^[AC][GT]AC
AflIII 212 ACGTGT A^C[GA][CT]GT
AlwI 918 GATCC GGATC(4/5)
Asp700I 972 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 236 GGTCC G^G[AT]CC
BalI 314 TGGCCA TGG^CCA
BbeI 148 GGCGCC GGCGC^C
BbsI 723 GAAGAC GAAGAC(2/6)
BfaI 22 CTAG C^TAG
BglI 557 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 723 GAAGAC GAAGAC(2/6)
BsaI 535 GAGACC GGTCTC(1/5)
BsiEI 26 CGATCG CG[GA][CT]^CG
BsmFI 14 GGGAC GGGAC(10/14)
Bsp1407I 215 TGTACA T^GTACA
BsrGI 215 TGTACA T^GTACA
BssHII 391 GCGCGC G^CGCGC
BstBI 613 TTCGAA TT^CGAA
BstYI 917 AGATCC [GA]^GATC[CT]
DraII 683 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 259 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 683 AGGCCCC [GA]G^G[ACGT]CC[CT]
HaeII 148 GGCGCC [GA]GCGC^[CT]
HincII 778 GTCAAC GT[CT]^[GA]AC
HindII 778 GTCAAC GT[CT]^[GA]AC
HinfI 51 GACTC G^A[ACGT]TC
KasI 148 GGCGCC G^GCGCC
KspI 490 CCGCGG CCGC^GG
MaeI 22 CTAG C^TAG
MflI 917 AGATCC [GA]^GATC[CT]
MluNI 314 TGGCCA TGG^CCA
MscI 314 TGGCCA TGG^CCA
NarI 148 GGCGCC GG^CGCC
NcoI 232 CCATGG C^CATGG
NspV 613 TTCGAA TT^CGAA
PflMI 345 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 51 GACTC GAGTC(4/5)
PstI 796 CTGCAG CTGCA^G
PvuI 26 CGATCG CGAT^CG
SacI 607 GAGCTC GAGCT^C
SacII 490 CCGCGG CCGC^GG
SapI 609 GCTCTTC GCTCTTC(1/4)
ScaI 164 AGTACT AGT^ACT
SfoI 148 GGCGCC GGC^GCC
SfuI 613 TTCGAA TT^CGAA
SspBI 215 TGTACA T^GTACA
StuI 809 AGGCCT AGG^CCT
Van91I 345 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 251 CCACCATCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 917 AGATCC [GA]^GATC[CT]
XmnI 972 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 78 GAGCAC G[AT]GC[AT]^C
607 GAGCTC
AviII 200 TGCGCA TGC^GCA
623 TGCGCA
BanII 607 GAGCTC G[GA]GC[CT]^C
892 GAGCCC
BcnI 145 CCCGG CC^[GC]GG
285 CCCGG
BsiHKAI 78 GAGCAC G[AT]GC[AT]^C
607 GAGCTC
BspMI 428 ACCTGC ACCTGC(4/8)
782 ACCTGC
BsrFI 409 ACCGGC [GA]^CCGG[CT]
743 ACCGGC
BsrI 347 ACTGG ACTGG(1/-1)
949 CCAGT
BstXI 254 CCATCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
914 CCAAGATCCTGG
Cfr10I 409 ACCGGC [GA]^CCGG[CT]
743 ACCGGC
EaeI 314 TGGCCA [CT]^GGCC[GA]
745 CGGCCA
EcoT14I 232 CCATGG C^C[AT][AT]GG
896 CCAAGG
FspI 200 TGCGCA TGC^GCA
623 TGCGCA
MslI 106 CATAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
255 CATCCACGTG
MspA1I 382 CCGCTG C[AC]G^C[GT]G
490 CCGCGG
NciI 145 CCCGG CC^[GC]GG
285 CCCGG
StyI 232 CCATGG C^C[AT][AT]GG
896 CCAAGG
TaqI 614 TCGA T^CGA
864 TCGA
TthHB8I 614 TCGA T^CGA
864 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BanI BbrPI BbvI BmyI BpmI
BsaAI BsaHI BsaJI BsgI BsiYI
BslI BsmAI BsmBI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DpnII DsaI EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PmaCI PmlI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TseI Tsp45I
TspRI