Restriction map for
pF1KB8203
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AlwI AocI ApaLI ApoI
AseI AsnI Asp700I Asp718I AspI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI Bst1107I BstAPI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MaeI MamI MfeI MflI MluI
MroI MseI MunI NarI NcoI
NdeI NheI NotI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AhdI 268 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 581 GGGCCC GGGCC^C
AscI 251 GGCGCGCC GG^CGCGCC
AspEI 268 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 368 TGGCCA TGG^CCA
BglI 583 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 481 CTGCAC GTGCAG(16/14)
BsmAI 674 GAGAC GTCTC(1/5)
BsmFI 395 GGGAC GGGAC(10/14)
BsmI 874 GCATTC GAATGC(1/-1)
BspMI 779 ACCTGC ACCTGC(4/8)
BsrBI 454 GAGCGG CCGCTC(-3/-3)
BsrDI 504 CATTGC GCAATG(2/0)
BsrFI 248 GCCGGC [GA]^CCGG[CT]
BsrI 740 CCAGT ACTGG(1/-1)
BstBI 416 TTCGAA TT^CGAA
Cfr10I 248 GCCGGC [GA]^CCGG[CT]
DraII 233 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 568 GACAGCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 509 CCGCGG C^C[GA][CT]GG
EagI 147 CGGCCG C^GGCCG
Eam1105I 268 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 147 CGGCCG C^GGCCG
Eco52I 147 CGGCCG C^GGCCG
EcoO109I 233 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 371 CCAAGG C^C[AT][AT]GG
HgaI 871 GACGC GACGC(5/10)
HinfI 419 GAATC G^A[ACGT]TC
KspI 509 CCGCGG CCGC^GG
MaeII 575 ACGT A^CGT
MaeIII 808 GTGAC ^GT[ACGT]AC
MluNI 368 TGGCCA TGG^CCA
MscI 368 TGGCCA TGG^CCA
MslI 550 CACGAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 248 GCCGGC GCC^GGC
NgoMIV 248 GCCGGC G^CCGGC
NruI 422 TCGCGA TCG^CGA
NspV 416 TTCGAA TT^CGAA
PvuII 310 CAGCTG CAG^CTG
SacI 82 GAGCTC GAGCT^C
SacII 509 CCGCGG CCGC^GG
SfiI 582 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 416 TTCGAA TT^CGAA
SmlI 85 CTCAAG C^T[CT][GA]AG
StuI 590 AGGCCT AGG^CCT
StyI 371 CCAAGG C^C[AT][AT]GG
TfiI 419 GAATC G^A[AT]TC
Tsp45I 808 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 123 GTAC GT^AC
618 GTAC
AlwNI 232 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
379 CAGCGGCTG
Aor51HI 409 AGCGCT AGC^GCT
434 AGCGCT
AspHI 82 GAGCTC G[AT]GC[AT]^C
775 GAGCAC
AvaI 58 CCCGAG C^[CT]CG[GA]G
69 CCCGAG
BcgI 93 GCAGGGCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
761 GCAAGGACATCG
BsiEI 147 CGGCCG CG[GA][CT]^CG
756 CGACCG
BsiHKAI 82 GAGCTC G[AT]GC[AT]^C
775 GAGCAC
BsoBI 58 CCCGAG C^[CT]CG[GA]G
69 CCCGAG
BssHII 74 GCGCGC G^CGCGC
252 GCGCGC
BssSI 226 CACGAG CACGAG(-5/-1)
550 CACGAG
DdeI 647 CTCAG C^T[ACGT]AG
884 CTGAG
DpnII 156 GATC ^GATC
702 GATC
Eco47III 409 AGCGCT AGC^GCT
434 AGCGCT
EcoNI 366 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
822 CCTGACCCAGG
FauI 168 CCCGC CCCGC(4/6)
720 GCGGG
MboI 156 GATC ^GATC
702 GATC
NdeII 156 GATC ^GATC
702 GATC
NlaIII 621 CATG CATG^
630 CATG
PstI 349 CTGCAG CTGCA^G
781 CTGCAG
RsaI 123 GTAC GT^AC
618 GTAC
SapI 63 GAAGAGC GCTCTTC(1/4)
412 GCTCTTC
Sau3AI 156 GATC ^GATC
702 GATC
SfcI 349 CTGCAG C^T[GA][CT]AG
781 CTGCAG
TaqI 417 TCGA T^CGA
770 TCGA
TspRI 304 CAGTG CA[GC]TG[ACGT][ACGT]^
741 CAGTG
TthHB8I 417 TCGA T^CGA
770 TCGA
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AluI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
EaeI EarI EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV Sau96I ScrFI
SfaNI TseI