Restriction map for
pF1KE2304
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DpnII DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeII
MamI MboI MfeI MflI MluI
MroI MspI MunI NaeI NarI
NciI NcoI NdeII NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 594 CGCG CG^CG
AciI 699 GCGG CCGC(-3/-1)
AfaI 498 GTAC GT^AC
AvrII 30 CCTAGG C^CTAGG
BalI 119 TGGCCA TGG^CCA
BbvI 90 GCAGC GCAGC(8/12)
BglI 26 GCCTCCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 30 CCTAGG C^CTAGG
BpmI 398 CTCCAG CTGGAG(16/14)
BseRI 544 GAGGAG GAGGAG(10/8)
BsiYI 615 CCGTGGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 615 CCGTGGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 324 GTCTC GTCTC(1/5)
BsmI 726 GCATTC GAATGC(1/-1)
BspHI 867 TCATGA T^CATGA
BspMI 374 ACCTGC ACCTGC(4/8)
BsrDI 719 GCAATG GCAATG(2/0)
BstUI 594 CGCG CG^CG
Cac8I 378 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 595 GCGC GCG^C
DraII 584 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 584 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 772 GAATTC G^AATTC
EcoT14I 30 CCTAGG C^C[AT][AT]GG
Fnu4HI 90 GCAGC GC^[ACGT]GC
HhaI 595 GCGC GCG^C
HinP1I 595 GCGC G^CGC
ItaI 90 GCAGC GC^[ACGT]GC
MaeIII 355 GTCAC ^GT[ACGT]AC
MluNI 119 TGGCCA TGG^CCA
MscI 119 TGGCCA TGG^CCA
MseI 56 TTAA T^TAA
MspA1I 643 CAGCTG C[AC]G^C[GT]G
MvnI 594 CGCG CG^CG
NdeI 422 CATATG CA^TATG
PpuMI 584 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 455 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 643 CAGCTG CAG^CTG
RcaI 867 TCATGA T^CATGA
RsaI 498 GTAC GT^AC
SfcI 509 CTACAG C^T[GA][CT]AG
StyI 30 CCTAGG C^C[AT][AT]GG
TaqI 271 TCGA T^CGA
Tru9I 56 TTAA T^TAA
TseI 90 GCAGC G^C[AT]GC
Tsp45I 355 GTCAC ^GT[GC]AC
TthHB8I 271 TCGA T^CGA
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccI 415 GTAGAC GT^[AC][GT]AC
817 GTAGAC
AluI 92 AGCT AG^CT
644 AGCT
AlwNI 110 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
583 CAGGACCTG
AvaII 372 GGACC G^G[AT]CC
585 GGACC
BfaI 31 CTAG C^TAG
163 CTAG
BsmFI 608 GGGAC GGGAC(10/14)
406 GTCCC
BssKI 255 CCAGG ^CC[ACGT]GG
582 CCAGG
BstNI 255 CCAGG CC^[AT]GG
582 CCAGG
Cfr13I 372 GGACC G^G[ACGT]CC
585 GGACC
DsaI 368 CCACGG C^C[GA][CT]GG
615 CCGTGG
EaeI 119 TGGCCA [CT]^GGCC[GA]
429 TGGCCG
EcoRII 255 CCAGG ^CC[AT]GG
582 CCAGG
MaeI 31 CTAG C^TAG
163 CTAG
MvaI 255 CCAGG CC^[AT]GG
582 CCAGG
MwoI 26 GCCTCCTAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
559 GCATACAAGGC
Sau96I 372 GGACC G^G[ACGT]CC
585 GGACC
ScrFI 255 CCAGG CC^[ACGT]GG
582 CCAGG
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AcsI ApoI BsaJI BsrI BstF5I
DdeI FokI HaeIII HinfI HphI
MboII MnlI MslI NlaIII PleI
SfaNI TfiI Tsp509I TspRI