Restriction map for
pF1KB5920
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AclI AcsI AcyI
AflII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspI AviII AvrII BbeI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsiEI BsiWI
BsmBI BspDI BspHI BspLU11I BssHII
Bst1107I BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HpaI KasI KpnI MamI
MfeI MluI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI ScaI SexAI SfiI SfoI
SgrAI SnaBI SpeI SphI SspI
SwaI TfiI Tru9I Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1351 GTAGAC GT^[AC][GT]AC
AhdI 478 GACCACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 56 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 106 CCTGAGG CC^T[ACGT]AGG
ApaI 255 GGGCCC GGGCC^C
Asp700I 546 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 478 GACCACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 801 GGATCC G^GATCC
BbrPI 376 CACGTG CAC^GTG
BbvCI 1227 CCTCAGC CCTCAGC(-5/-2)
BcgI 806 CGAGGCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 454 CTAG C^TAG
BglI 761 GCCGATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmI 672 GAATGC GAATGC(1/-1)
BsrBI 463 GAGCGG CCGCTC(-3/-3)
BsrDI 1573 CATTGC GCAATG(2/0)
BsrFI 148 ACCGGC [GA]^CCGG[CT]
BstAPI 355 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 923 TTCGAA TT^CGAA
Bsu36I 106 CCTGAGG CC^T[ACGT]AGG
Cfr10I 148 ACCGGC [GA]^CCGG[CT]
Eam1105I 478 GACCACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1003 CTCTTC CTCTTC(1/4)
Eco81I 106 CCTGAGG CC^T[ACGT]AGG
EcoNI 1185 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 858 GACGC GACGC(5/10)
HphI 855 GGTGA GGTGA(8/7)
Ksp632I 1003 CTCTTC CTCTTC(1/4)
KspI 1545 CCGCGG CCGC^GG
MaeI 454 CTAG C^TAG
MaeIII 856 GTGAC ^GT[ACGT]AC
NcoI 1121 CCATGG C^CATGG
NspV 923 TTCGAA TT^CGAA
PflMI 55 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 376 CACGTG CAC^GTG
PmlI 376 CACGTG CAC^GTG
SacII 1545 CCGCGG CCGC^GG
SbfI 335 CCTGCAGG CCTGCA^GG
SfuI 923 TTCGAA TT^CGAA
SmaI 1163 CCCGGG CCC^GGG
Sse8387I 335 CCTGCAGG CCTGCA^GG
StuI 1199 AGGCCT AGG^CCT
Tsp45I 856 GTGAC ^GT[GC]AC
Van91I 55 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1163 CCCGGG C^CCGGG
XmaI 1163 CCCGGG C^CCGGG
XmnI 546 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccIII 39 TCCGGA T^CCGGA
542 TCCGGA
AflIII 377 ACGTGT A^C[GA][CT]GT
845 ACGTGT
AvaI 250 CTCGGG C^[CT]CG[GA]G
1163 CCCGGG
BsaI 421 GAGACC GGTCTC(1/5)
609 GGTCTC
BsaWI 39 TCCGGA [AT]^CCGG[AT]
542 TCCGGA
BseAI 39 TCCGGA T^CCGGA
542 TCCGGA
BsoBI 250 CTCGGG C^[CT]CG[GA]G
1163 CCCGGG
Bsp1407I 317 TGTACA T^GTACA
1025 TGTACA
BspEI 39 TCCGGA T^CCGGA
542 TCCGGA
BsrGI 317 TGTACA T^GTACA
1025 TGTACA
EcoT14I 785 CCAAGG C^C[AT][AT]GG
1121 CCATGG
HindIII 730 AAGCTT A^AGCTT
919 AAGCTT
MroI 39 TCCGGA T^CCGGA
542 TCCGGA
MslI 376 CACGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1581 CACGCGGGTG
PpuMI 64 GGGACCC [GA]G^G[AT]CC[CT]
121 AGGTCCC
SmlI 411 CTTGAG C^T[CT][GA]AG
1089 CTTGAG
SspBI 317 TGTACA T^GTACA
1025 TGTACA
StyI 785 CCAAGG C^C[AT][AT]GG
1121 CCATGG
Tsp509I 246 AATT ^AATT
1033 AATT
XcmI 842 CCAACGTGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1323 CCAGCGGCTTATTGG
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BalI BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BssSI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI TspRI TthHB8I XhoII