Restriction map for
pF1KB5356
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# NO CUT ENZYMES ( 132 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmAI BsmBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HpaI KpnI KspI
MamI MfeI MflI MluI MroI
MseI MunI NaeI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AatII 51 GACGTC GACGT^C
BbeI 769 GGCGCC GGCGC^C
BfaI 721 CTAG C^TAG
BglI 92 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmI 824 GAATGC GAATGC(1/-1)
BsrBI 46 GAGCGG CCGCTC(-3/-3)
BssSI 790 CTCGTG CACGAG(-5/-1)
BstAPI 644 GCATGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 458 CCATCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 285 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 181 GACCGCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI 440 GCGTC GACGC(5/10)
HindIII 1081 AAGCTT A^AGCTT
HinfI 976 GACTC G^A[ACGT]TC
KasI 769 GGCGCC G^GCGCC
MaeI 721 CTAG C^TAG
MaeIII 283 GTCAC ^GT[ACGT]AC
NarI 769 GGCGCC GG^CGCC
NotI 210 GCGGCCGC GC^GGCCGC
NspI 258 GCATGT [GA]CATG^[CT]
PleI 976 GACTC GAGTC(4/5)
PpuMI 173 AGGACCC [GA]G^G[AT]CC[CT]
PstI 679 CTGCAG CTGCA^G
SapI 229 GAAGAGC GCTCTTC(1/4)
SexAI 1295 ACCTGGT A^CC[AT]GGT
SfoI 769 GGCGCC GGC^GCC
SmaI 205 CCCGGG CCC^GGG
SmlI 574 CTCAAG C^T[CT][GA]AG
Tsp45I 283 GTCAC ^GT[GC]AC
XmaCI 205 CCCGGG C^CCGGG
XmaI 205 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccII 83 CGCG CG^CG
753 CGCG
AcyI 51 GACGTC G[GA]^CG[CT]C
769 GGCGCC
AfaI 483 GTAC GT^AC
735 GTAC
ApaI 108 GGGCCC GGGCC^C
666 GGGCCC
AspHI 472 GTGCTC G[AT]GC[AT]^C
571 GTGCTC
AvaI 205 CCCGGG C^[CT]CG[GA]G
1090 CTCGGG
BalI 590 TGGCCA TGG^CCA
1178 TGGCCA
BanI 769 GGCGCC G^G[CT][GA]CC
937 GGTGCC
BbsI 973 GAAGAC GAAGAC(2/6)
522 GTCTTC
BpuAI 973 GAAGAC GAAGAC(2/6)
522 GTCTTC
BsaHI 51 GACGTC G[GA]^CG[CT]C
769 GGCGCC
BsiHKAI 472 GTGCTC G[AT]GC[AT]^C
571 GTGCTC
BsoBI 205 CCCGGG C^[CT]CG[GA]G
1090 CTCGGG
BspHI 431 TCATGA T^CATGA
698 TCATGA
BstUI 83 CGCG CG^CG
753 CGCG
FauI 1240 CCCGC CCCGC(4/6)
1145 GCGGG
HapII 206 CCGG C^CGG
961 CCGG
Hin1I 51 GACGTC G[GA]^CG[CT]C
769 GGCGCC
HpaII 206 CCGG C^CGG
961 CCGG
HphI 166 GGTGA GGTGA(8/7)
548 TCACC
MaeII 52 ACGT A^CGT
1261 ACGT
MluNI 590 TGGCCA TGG^CCA
1178 TGGCCA
MscI 590 TGGCCA TGG^CCA
1178 TGGCCA
MspA1I 486 CCGCTG C[AC]G^C[GT]G
1143 CAGCGG
MspI 206 CCGG C^CGG
961 CCGG
MvnI 83 CGCG CG^CG
753 CGCG
PflMI 112 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
890 CCATGGTGTGG
RcaI 431 TCATGA T^CATGA
698 TCATGA
RsaI 483 GTAC GT^AC
735 GTAC
SfaNI 531 GATGC GCATC(5/9)
653 GCATC
StuI 219 AGGCCT AGG^CCT
686 AGGCCT
Van91I 112 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
890 CCATGGTGTGG
XcmI 458 CCATCTTCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1286 CCACGGACAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BclI BcnI BmyI BpmI BsaJI
BseRI BsiEI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeII HaeIII
HhaI HinP1I ItaI Ksp632I MboI
MboII MnlI MslI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfcI StyI
TseI TspRI