Restriction map for
pF1KB3649
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AviII AvrII
BalI BamHI BanII BbeI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BmyI BsaHI BsaI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Cac8I Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI Hin1I HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 1733 AAATTT [GA]^AATT[CT]
AocI 1015 CCTGAGG CC^T[ACGT]AGG
ApoI 1733 AAATTT [GA]^AATT[CT]
AspI 106 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 484 GGTGCC G^G[CT][GA]CC
BbrPI 818 CACGTG CAC^GTG
BfaI 1073 CTAG C^TAG
BlpI 1393 GCTGAGC GC^T[ACGT]AGC
BpmI 185 CTCCAG CTGGAG(16/14)
Bpu1102I 1393 GCTGAGC GC^T[ACGT]AGC
BsaBI 667 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 598 CTGCAC GTGCAG(16/14)
BsmAI 369 GTCTC GTCTC(1/5)
BspHI 60 TCATGA T^CATGA
Bsu36I 1015 CCTGAGG CC^T[ACGT]AGG
CelII 1393 GCTGAGC GC^T[ACGT]AGC
CfoI 1437 GCGC GCG^C
DsaI 1441 CCGTGG C^C[GA][CT]GG
Eco81I 1015 CCTGAGG CC^T[ACGT]AGG
EcoT14I 167 CCAAGG C^C[AT][AT]GG
HaeII 1436 AGCGCC [GA]GCGC^[CT]
HgaI 1003 GACGC GACGC(5/10)
HhaI 1437 GCGC GCG^C
HinP1I 1437 GCGC G^CGC
HincII 754 GTTGAC GT[CT]^[GA]AC
HindII 754 GTTGAC GT[CT]^[GA]AC
HindIII 337 AAGCTT A^AGCTT
MaeI 1073 CTAG C^TAG
MamI 667 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 455 TTAA T^TAA
MslI 70 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1427 CAGCTG C[AC]G^C[GT]G
NspI 651 ACATGC [GA]CATG^[CT]
PflFI 106 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 759 CCAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 818 CACGTG CAC^GTG
PmlI 818 CACGTG CAC^GTG
PpuMI 830 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1427 CAGCTG CAG^CTG
RcaI 60 TCATGA T^CATGA
SapI 1432 GAAGAGC GCTCTTC(1/4)
SfcI 1469 CTGTAG C^T[GA][CT]AG
SnaBI 988 TACGTA TAC^GTA
StyI 167 CCAAGG C^C[AT][AT]GG
Tru9I 455 TTAA T^TAA
Tth111I 106 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 759 CCAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 987 GTAC GT^AC
991 GTAC
AlwNI 887 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1085 CAGCTCCTG
AvaI 371 CTCGGG C^[CT]CG[GA]G
850 CCCGAG
BglII 672 AGATCT A^GATCT
1227 AGATCT
BsaAI 818 CACGTG [CT]AC^GT[GA]
988 TACGTA
BsaWI 842 ACCGGA [AT]^CCGG[AT]
1563 ACCGGA
BsoBI 371 CTCGGG C^[CT]CG[GA]G
850 CCCGAG
BstXI 901 CCAGATGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1526 CCAGTGGTATGG
DraII 830 AGGACCC [GA]G^G[ACGT]CC[CT]
1364 GGGGCCT
EcoO109I 830 AGGACCC [GA]G^G[ACGT]CC[CT]
1364 GGGGCCT
HapII 843 CCGG C^CGG
1564 CCGG
HpaII 843 CCGG C^CGG
1564 CCGG
MaeII 819 ACGT A^CGT
989 ACGT
MspI 843 CCGG C^CGG
1564 CCGG
RsaI 987 GTAC GT^AC
991 GTAC
TaqI 1329 TCGA T^CGA
1421 TCGA
TthHB8I 1329 TCGA T^CGA
1421 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AluI AlwI AvaII BbsI BbvI
BpuAI BsaJI BseRI BsiYI BslI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI TfiI TseI Tsp509I
TspRI XhoII