Restriction map for
pF1KE0799
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvrII
BanI BbeI BbsI BbvCI BcgI
BciVI BfaI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstUI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
FseI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MamI MluI MroI MslI MspA1I
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AocI 8 CCTCAGG CC^T[ACGT]AGG
AspHI 142 GAGCTC G[AT]GC[AT]^C
AvaII 1207 GGACC G^G[AT]CC
AviII 1181 TGCGCA TGC^GCA
BalI 489 TGGCCA TGG^CCA
BamHI 220 GGATCC G^GATCC
BbrPI 610 CACGTG CAC^GTG
BcnI 773 CCGGG CC^[GC]GG
BglII 65 AGATCT A^GATCT
BsaAI 610 CACGTG [CT]AC^GT[GA]
BsiHKAI 142 GAGCTC G[AT]GC[AT]^C
BspHI 1223 TCATGA T^CATGA
BspMI 400 GCAGGT ACCTGC(4/8)
BsrBI 973 GAGCGG CCGCTC(-3/-3)
BstBI 959 TTCGAA TT^CGAA
BstEII 795 GGTGACC G^GT[ACGT]ACC
BstPI 795 GGTGACC G^GT[ACGT]ACC
Bsu36I 8 CCTCAGG CC^T[ACGT]AGG
DraI 1231 TTTAAA TTT^AAA
EaeI 489 TGGCCA [CT]^GGCC[GA]
Eco81I 8 CCTCAGG CC^T[ACGT]AGG
EcoNI 679 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 795 GGTGACC G^GT[ACGT]ACC
EcoT22I 350 ATGCAT ATGCA^T
FauI 975 GCGGG CCCGC(4/6)
FspI 1181 TGCGCA TGC^GCA
HapII 773 CCGG C^CGG
HincII 178 GTTGAC GT[CT]^[GA]AC
HindII 178 GTTGAC GT[CT]^[GA]AC
HpaII 773 CCGG C^CGG
MfeI 437 CAATTG C^AATTG
MluNI 489 TGGCCA TGG^CCA
MscI 489 TGGCCA TGG^CCA
MspI 773 CCGG C^CGG
MunI 437 CAATTG C^AATTG
NciI 773 CCGGG CC^[GC]GG
NcoI 876 CCATGG C^CATGG
NsiI 350 ATGCAT ATGCA^T
NspV 959 TTCGAA TT^CGAA
PflMI 436 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 610 CACGTG CAC^GTG
PmlI 610 CACGTG CAC^GTG
PstI 1339 CTGCAG CTGCA^G
RcaI 1223 TCATGA T^CATGA
SacI 142 GAGCTC GAGCT^C
SfuI 959 TTCGAA TT^CGAA
SmlI 1022 CTCAAG C^T[CT][GA]AG
SspI 658 AATATT AAT^ATT
Van91I 436 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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BanII 142 GAGCTC G[GA]GC[CT]^C
819 GAGCCC
BclI 758 TGATCA T^GATCA
1250 TGATCA
BpmI 647 CTGGAG CTGGAG(16/14)
802 CTGGAG
BsrDI 386 CATTGC GCAATG(2/0)
1185 CATTGC
CfoI 390 GCGC GCG^C
1182 GCGC
DraII 540 AGGGCCT [GA]G^G[ACGT]CC[CT]
1026 AGGCCCT
DrdI 22 GACTTACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
312 GACAATGGAGTC
EarI 789 GAAGAG CTCTTC(1/4)
1424 GAAGAG
EcoO109I 540 AGGGCCT [GA]G^G[ACGT]CC[CT]
1026 AGGCCCT
FbaI 758 TGATCA T^GATCA
1250 TGATCA
HhaI 390 GCGC GCG^C
1182 GCGC
HinP1I 390 GCGC G^CGC
1182 GCGC
Ksp632I 789 GAAGAG CTCTTC(1/4)
1424 GAAGAG
MaeII 611 ACGT A^CGT
1247 ACGT
NlaIV 220 GGATCC GG[ACGT]^[ACGT]CC
1093 GGGGCC
PleI 319 GAGTC GAGTC(4/5)
1016 GAGTC
SfcI 680 CTACAG C^T[GA][CT]AG
1339 CTGCAG
TaqI 287 TCGA T^CGA
960 TCGA
TthHB8I 287 TCGA T^CGA
960 TCGA
XcmI 920 CCAGGATTTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1386 CCAGTGCCCCTTTGG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BmyI BsaJI BseRI
BsgI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII