Restriction map for
pF1KE0696
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbvCI
BfrI BglI BlnI BlpI BmyI
BpmI Bpu1102I BsaBI BseAI BseRI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HpaI KpnI KspI
MamI MluI MluNI MroI MscI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 1174 GTAGAC GT^[AC][GT]AC
AccII 697 CGCG CG^CG
AcsI 70 AAATTT [GA]^AATT[CT]
AcyI 856 GGCGCC G[GA]^CG[CT]C
AflIII 162 ACGTGT A^C[GA][CT]GT
AlwNI 848 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1150 CCTCAGG CC^T[ACGT]AGG
ApoI 70 AAATTT [GA]^AATT[CT]
BanI 856 GGCGCC G^G[CT][GA]CC
BbeI 856 GGCGCC GGCGC^C
BbrPI 161 CACGTG CAC^GTG
BbsI 894 GTCTTC GAAGAC(2/6)
BcgI 1035 CGAGTCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 96 GGATAC GTATCC(6/5)
BclI 285 TGATCA T^GATCA
BglII 886 AGATCT A^GATCT
BpuAI 894 GTCTTC GAAGAC(2/6)
BsaAI 161 CACGTG [CT]AC^GT[GA]
BsaHI 856 GGCGCC G[GA]^CG[CT]C
BsaI 999 GGTCTC GGTCTC(1/5)
BsaWI 1197 TCCGGT [AT]^CCGG[AT]
BsgI 805 GTGCAG GTGCAG(16/14)
BsmBI 788 CGTCTC CGTCTC(1/5)
BsmI 249 GAATGC GAATGC(1/-1)
BstBI 77 TTCGAA TT^CGAA
BstUI 697 CGCG CG^CG
BstYI 886 AGATCT [GA]^GATC[CT]
Bsu36I 1150 CCTCAGG CC^T[ACGT]AGG
CfoI 857 GCGC GCG^C
Eco81I 1150 CCTCAGG CC^T[ACGT]AGG
FauI 873 GCGGG CCCGC(4/6)
FbaI 285 TGATCA T^GATCA
HaeII 856 GGCGCC [GA]GCGC^[CT]
HgaI 892 GCGTC GACGC(5/10)
HhaI 857 GCGC GCG^C
Hin1I 856 GGCGCC G[GA]^CG[CT]C
HinP1I 857 GCGC G^CGC
HindIII 178 AAGCTT A^AGCTT
KasI 856 GGCGCC G^GCGCC
MfeI 640 CAATTG C^AATTG
MflI 886 AGATCT [GA]^GATC[CT]
MslI 750 CATAATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 871 CAGCGG C[AC]G^C[GT]G
MunI 640 CAATTG C^AATTG
MvnI 697 CGCG CG^CG
NarI 856 GGCGCC GG^CGCC
NspV 77 TTCGAA TT^CGAA
PmaCI 161 CACGTG CAC^GTG
PmlI 161 CACGTG CAC^GTG
ScaI 266 AGTACT AGT^ACT
SfoI 856 GGCGCC GGC^GCC
SfuI 77 TTCGAA TT^CGAA
SmlI 560 CTCAAG C^T[CT][GA]AG
XbaI 39 TCTAGA T^CTAGA
XcmI 390 CCATGGTGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 886 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 12 GGATC GGATC(4/5)
154 GGATC
Asp700I 264 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
688 GAACCTCTTC
BbvI 952 GCAGC GCAGC(8/12)
986 GCAGC
BcnI 880 CCCGG CC^[GC]GG
910 CCCGG
BsmFI 761 GGGAC GGGAC(10/14)
878 GTCCC
BstF5I 904 CATCC GGATG(2/0)
645 GGATG
BstNI 729 CCAGG CC^[AT]GG
1057 CCAGG
Cac8I 807 GCAGGC GC[ACGT]^[ACGT]GC
1189 GCGAGC
DraII 293 AGGACCT [GA]G^G[ACGT]CC[CT]
876 GGGTCCC
DsaI 390 CCATGG C^C[GA][CT]GG
1013 CCATGG
EcoO109I 293 AGGACCT [GA]G^G[ACGT]CC[CT]
876 GGGTCCC
EcoRII 729 CCAGG ^CC[AT]GG
1057 CCAGG
Fnu4HI 952 GCAGC GC^[ACGT]GC
986 GCAGC
FokI 904 CATCC GGATG(9/13)
645 GGATG
HaeIII 221 GGCC GG^CC
1147 GGCC
HphI 394 GGTGA GGTGA(8/7)
490 GGTGA
ItaI 952 GCAGC GC^[ACGT]GC
986 GCAGC
MvaI 729 CCAGG CC^[AT]GG
1057 CCAGG
MwoI 859 GCCATTGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
952 GCAGCCACCGC
NciI 880 CCCGG CC^[GC]GG
910 CCCGG
NcoI 390 CCATGG C^CATGG
1013 CCATGG
PleI 358 GACTC GAGTC(4/5)
1036 GAGTC
PpuMI 293 AGGACCT [GA]G^G[AT]CC[CT]
876 GGGTCCC
TaqI 78 TCGA T^CGA
792 TCGA
TfiI 21 GAATC G^A[AT]TC
922 GAATC
TseI 952 GCAGC G^C[AT]GC
986 GCAGC
TthHB8I 78 TCGA T^CGA
792 TCGA
XmnI 264 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
688 GAACCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AvaII BfaI
BsaJI BsmAI BsrI BssKI Cfr13I
DdeI DpnII EarI EcoT14I HapII
HinfI HpaII Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
Tru9I Tsp45I Tsp509I TspRI