Restriction map for
pF1KB8910
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI DsaI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FokI
FseI FspI HindIII HpaI HphI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MunI NaeI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SapI SbfI ScaI
SfcI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tsp509I Tth111I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 235 GTCGAC GT^[AC][GT]AC
AcyI 212 GGCGCC G[GA]^CG[CT]C
AfaI 529 GTAC GT^AC
AlwNI 495 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 212 GGCGCC G^G[CT][GA]CC
BbeI 212 GGCGCC GGCGC^C
BbsI 283 GAAGAC GAAGAC(2/6)
BfaI 127 CTAG C^TAG
BpuAI 283 GAAGAC GAAGAC(2/6)
BsaHI 212 GGCGCC G[GA]^CG[CT]C
BsiEI 260 CGGCCG CG[GA][CT]^CG
BsmAI 361 GTCTC GTCTC(1/5)
BsmFI 579 GTCCC GGGAC(10/14)
BspMI 221 ACCTGC ACCTGC(4/8)
BsrBI 120 GAGCGG CCGCTC(-3/-3)
BstXI 140 CCATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 375 GATC ^GATC
DraII 496 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 260 CGGCCG [CT]^GGCC[GA]
EagI 260 CGGCCG C^GGCCG
EarI 133 CTCTTC CTCTTC(1/4)
EclXI 260 CGGCCG C^GGCCG
Eco52I 260 CGGCCG C^GGCCG
EcoO109I 496 AGGTCCT [GA]G^G[ACGT]CC[CT]
HaeII 212 GGCGCC [GA]GCGC^[CT]
Hin1I 212 GGCGCC G[GA]^CG[CT]C
HincII 235 GTCGAC GT[CT]^[GA]AC
HindII 235 GTCGAC GT[CT]^[GA]AC
KasI 212 GGCGCC G^GCGCC
Ksp632I 133 CTCTTC CTCTTC(1/4)
MaeI 127 CTAG C^TAG
MaeII 305 ACGT A^CGT
MboI 375 GATC ^GATC
MseI 302 TTAA T^TAA
NarI 212 GGCGCC GG^CGCC
NdeI 309 CATATG CA^TATG
NdeII 375 GATC ^GATC
PflMI 477 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 427 GAGTC GAGTC(4/5)
PpuMI 496 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 529 GTAC GT^AC
SalI 235 GTCGAC G^TCGAC
Sau3AI 375 GATC ^GATC
SfoI 212 GGCGCC GGC^GCC
SmaI 169 CCCGGG CCC^GGG
SmlI 67 CTCAAG C^T[CT][GA]AG
TfiI 437 GAATC G^A[AT]TC
Tru9I 302 TTAA T^TAA
Van91I 477 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 558 CCAGAACAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 169 CCCGGG C^CCGGG
XmaI 169 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AspHI 64 GAGCTC G[AT]GC[AT]^C
181 GAGCTC
AvaI 82 CTCGGG C^[CT]CG[GA]G
169 CCCGGG
AvaII 3 GGACC G^G[AT]CC
497 GGTCC
BsiHKAI 64 GAGCTC G[AT]GC[AT]^C
181 GAGCTC
BsoBI 82 CTCGGG C^[CT]CG[GA]G
169 CCCGGG
DraIII 25 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
478 CACCTGGTG
HapII 170 CCGG C^CGG
263 CCGG
HinfI 427 GAGTC G^A[ACGT]TC
437 GAATC
HpaII 170 CCGG C^CGG
263 CCGG
MaeIII 429 GTCAC ^GT[ACGT]AC
551 GTGAC
MspI 170 CCGG C^CGG
263 CCGG
SacI 64 GAGCTC GAGCT^C
181 GAGCTC
SfaNI 383 GCATC GCATC(5/9)
604 GCATC
Tsp45I 429 GTCAC ^GT[GC]AC
551 GTGAC
TspRI 431 CACTG CA[GC]TG[ACGT][ACGT]^
470 CAGTG
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AccII AciI AluI BanII BbvI
BcnI BmyI BpmI BsaJI BsgI
BsiYI BslI Bsp1286I BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
EcoRII FauI Fnu4HI HaeIII HgaI
HhaI HinP1I ItaI MboII MnlI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SexAI TaqI
TseI TthHB8I